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The mechanism underlying activation of factor IX by factor XIa (2014)
Journal Article
Gailani, D., Geng, Y., Verhamme, I., Sun, M., Bajaj, S. P., Messer, A., & Emsley, J. (2014). The mechanism underlying activation of factor IX by factor XIa. Thrombosis Research, 133(Supplement 1), S48-S51. doi:10.1016/j.thromres.2014.03.020

Factor XI (fXI) is the zymogen of a plasma protease, factor XIa (fXIa), that contributes to thrombin generation during blood coagulation by proteolytic conversion of factor IX (fIX) to factor IXa? (fIXa?). There is considerable interest in fXIa as a... Read More about The mechanism underlying activation of factor IX by factor XIa.

Effect of a toggle switch mutation in TM6 of the human adenosine A3 receptor on Gi protein-dependent signalling and Gi-independent receptor internalization (2014)
Journal Article
Stoddart, L. A., Kellam, B., Briddon, S. J., & Hill, S. J. (2014). Effect of a toggle switch mutation in TM6 of the human adenosine A3 receptor on Gi protein-dependent signalling and Gi-independent receptor internalization. British Journal of Pharmacology, 171(16), https://doi.org/10.1111/bph.12739

Background and Purpose: The highly conserved tryptophan (W6.48) in transmembrane domain 6 of GPCRs has been shown to play a central role in forming an active conformation in response to agonist binding. We set out to characterize the effect of this m... Read More about Effect of a toggle switch mutation in TM6 of the human adenosine A3 receptor on Gi protein-dependent signalling and Gi-independent receptor internalization.

The effect of the electronic transmission of prescriptions on dispensing errors and prescription enhancements made in English community pharmacies: a naturalistic stepped wedge study (2014)
Journal Article
Franklin, B. D., Reynolds, M., Sadler, S., Hibberd, R., Avery, A. J., Armstrong, S. J., …Barber, N. (2014). The effect of the electronic transmission of prescriptions on dispensing errors and prescription enhancements made in English community pharmacies: a naturalistic stepped wedge study. BMJ Quality and Safety, 23(8), https://doi.org/10.1136/bmjqs-2013-002776

Objectives: To compare prevalence and types of dispensing errors and pharmacists’ labelling enhancements, for prescriptions transmitted electronically versus paper prescriptions. Design: Naturalistic stepped wedge study. Setting: 15 English communi... Read More about The effect of the electronic transmission of prescriptions on dispensing errors and prescription enhancements made in English community pharmacies: a naturalistic stepped wedge study.

Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat (2014)
Journal Article
Montowska, M., Rao, W., Alexander, M. R., Tucker, G. A., & Barrett, D. A. (2014). Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat. Analytical Chemistry, 86(9), 4479-4487. https://doi.org/10.1021/ac5003432

The use of ambient desorption electrospray ionization (DESI-MS) mass spectrometry and liquid extraction surface analysis mass spectrometry (LESA-MS) is explored for the first time to analyse skeletal muscle proteins obtained from mixture of standard... Read More about Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat.

A novel technique for the production of electrospun scaffolds with tailored three-dimensional micro-patterns employing additive manufacturing (2014)
Journal Article
Rogers, C. M., Morris, G. E., Gould, T. W., Bail, R., Toumpaniari, S., Harrington, H., …Rose, F. R. A. J. (2014). A novel technique for the production of electrospun scaffolds with tailored three-dimensional micro-patterns employing additive manufacturing. Biofabrication, 6(3), 035003. https://doi.org/10.1088/1758-5082/6/3/035003

Electrospinning is a common technique used to fabricate fibrous scaffolds for tissue engineering applications. There is now growing interest in assessing the ability of collector plate design to influence the patterning of the fibres during the elect... Read More about A novel technique for the production of electrospun scaffolds with tailored three-dimensional micro-patterns employing additive manufacturing.

LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules (2014)
Book Chapter
Ortori, C. A., Ortori, C., Halliday, N., Cámara, M., Williams, P., & Barrett, D. A. (2014). LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules. In A. Filloux, & J. Ramos (Eds.), Pseudomonas Methods and Protocols (255-270). Springer. https://doi.org/10.1007/978-1-4939-0473-0_21

© Springer Science+Business Media New York 2014. Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N -acyl- L - homoserine lactones and 2-alkyl-4-(1 H)-quinolones (AQs), including the known quorum sensing signalli... Read More about LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules.

Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation (2014)
Journal Article
Dixon, J. E., Shah, D. A., Rogers, C., Hall, S., Weston, N., Parmenter, C. D., …Shakesheff, K. M. (2014). Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. Proceedings of the National Academy of Sciences, 111(15), 5580-5585. https://doi.org/10.1073/pnas.1319685111

The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the... Read More about Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation.

Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation (2014)
Journal Article
Harrington, H., Cato, P., Salazar, F., Wilkinson, M., Knox, A., Haycock, J. W., …Ghaemmaghami, A. M. (2014). Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation. Molecular Pharmaceutics, 11(7), 2082-2091. https://doi.org/10.1021/mp5000295

The development of more complex in vitro models for the assessment of novel drugs and chemicals is needed because of the limited biological relevance of animal models to humans as well as ethical considerations. Although some human-cell-based assays... Read More about Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation.

Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors (2014)
Journal Article
Murray, E. J., Crowley, R. C., Truman, A., Clarke, S. R., Cottam, J. A., Jadhav, G. P., …Williams, P. (2014). Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors. Journal of Medicinal Chemistry, 57(6), 2813-2819. https://doi.org/10.1021/jm500215s

A series of 3-oxo-C 12 -HSL, tetramic acid, and tetronic acid analogues were synthesized to gain insights into the structural requirements for quorum sensing inhibition in Staphylococcus aureus. Compounds active against agr were noncompetitive inhibi... Read More about Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors.

What proportion of prescription items dispensed in community pharmacies are eligible for the New Medicine Service? (2014)
Journal Article
Wells, K. M., Boyd, M. J., Thornley, T., & Boardman, H. F. (in press). What proportion of prescription items dispensed in community pharmacies are eligible for the New Medicine Service?. BMC Health Services Research, 14(1), https://doi.org/10.1186/1472-6963-14-115

Background: The payment structure for the New Medicine Service (NMS) in England is based on the assumption that 0.5% of prescription items dispensed in community pharmacies are eligible for the service. This assumption is based on a theoretical calcu... Read More about What proportion of prescription items dispensed in community pharmacies are eligible for the New Medicine Service?.

The evolving small-molecule fluorescent-conjugate toolbox for Class A GPCRs (2014)
Journal Article
Vernall, A. J., Hill, S. J., & Kellam, B. (2014). The evolving small-molecule fluorescent-conjugate toolbox for Class A GPCRs. British Journal of Pharmacology, 171(5), https://doi.org/10.1111/bph.12265

The past decade has witnessed fluorescently tagged drug molecules gaining significant attraction in their use as pharmacological tools with which to visualize and interrogate receptor targets at the single-cell level. Additionally, one can generate d... Read More about The evolving small-molecule fluorescent-conjugate toolbox for Class A GPCRs.

Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules (2014)
Journal Article
Stolnik-Trenkic, S., Vllasaliua, D., Falcone, F. H., & Garnett, M. (2014). Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules. Experimental Cell Research, 323(1), 218-231. https://doi.org/10.1016/j.yexcr.2014.02.022

This work examines the potential drug delivery barrier of the basement membrane (BM) by assessing the permeability of select macromolecules and nanoparticles. The study further extends to probing the effect of BM on intestinal epithelial cell attachm... Read More about Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules.

Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition (2014)
Journal Article
Magnusson, J. P., Alexander, C., Fernández-Trillo, F., Sicilia, G., & Spain, S. G. (2014). Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition. Nanoscale, 6(4), 2368-2374. https://doi.org/10.1039/c3nr04952c

Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA c... Read More about Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition.

Discovery of AZD3199, An Inhaled Ultralong Acting ?2 Receptor Agonist with Rapid Onset of Action (2014)
Journal Article
Stocks, M., Alcaraz, L., Bailey, A., Bonnert, R., Cadogan, E., Christie, J., …Young, A. (2014). Discovery of AZD3199, An Inhaled Ultralong Acting β2 Receptor Agonist with Rapid Onset of Action. ACS Medicinal Chemistry Letters, 5(4), 416-421. https://doi.org/10.1021/ml4005232

A series of dibasic des-hydroxy ?2 receptor agonists has been prepared and evaluated for potential as inhaled ultra-long acting bronchodilators. Determination of activities at the human ?-adrenoreceptors demonstrated a series of highly potent and se... Read More about Discovery of AZD3199, An Inhaled Ultralong Acting ?2 Receptor Agonist with Rapid Onset of Action.

Thermally Switchable Polymers Achieve Controlled Escherichia coli Detachment (2014)
Journal Article
Hook, A. L., Chang, C., Scurr, D. J., Langer, R., Anderson, D. G., Williams, P., …Alexander, M. R. (2014). Thermally Switchable Polymers Achieve Controlled Escherichia coli Detachment. Advanced Healthcare Materials, 3(7), 1020-1025. https://doi.org/10.1002/adhm.201300518

The thermally triggered release of up to 96% of attached uropathogenic E. coli is achieved on two polymers with opposite changes in surface wettability upon reduction in temperature. This demonstrates that the bacterial attachment to a surface cannot... Read More about Thermally Switchable Polymers Achieve Controlled Escherichia coli Detachment.

Disease acceptance and adherence to imatinib in Taiwanese chronic myeloid leukaemia outpatients (2014)
Journal Article
Chen, L., Chen, T., Huang, Y., & Chang, C. (2014). Disease acceptance and adherence to imatinib in Taiwanese chronic myeloid leukaemia outpatients. International Journal of Clinical Pharmacy, 36(1), https://doi.org/10.1007/s11096-013-9867-8

Background The launch of imatinib has turned chronic myeloid leukaemia (CML) into a chronic illness due to the dramatic improvement in survival. Several recent studies have demonstrated that poor adherence to imatinib may hamper the therapeutic outco... Read More about Disease acceptance and adherence to imatinib in Taiwanese chronic myeloid leukaemia outpatients.

Multimodal Polymer Nanoparticles with Combined 19F Magnetic Resonance and Optical Detection for Tunable, Targeted, Multimodal Imaging in Vivo (2014)
Journal Article
Rolfe, B. E., Blakey, I., Squires, O., Peng, H., Boase, N. R., Alexander, C., …Thurecht, K. J. (2014). Multimodal Polymer Nanoparticles with Combined 19F Magnetic Resonance and Optical Detection for Tunable, Targeted, Multimodal Imaging in Vivo. Journal of the American Chemical Society, 136(6), 2413-2419. https://doi.org/10.1021/ja410351h