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A novel technique for the production of electrospun scaffolds with tailored three-dimensional micro-patterns employing additive manufacturing

Rogers, Catherine M; Morris, Gavin E; Gould, Toby WA; Bail, Robert; Toumpaniari, Sotiria; Harrington, Helen; Dixon, James E; Shakesheff, Kevin M; Segal, Joel; Rose, Felicity R A J

A novel technique for the production of electrospun scaffolds with tailored three-dimensional micro-patterns employing additive manufacturing Thumbnail


Authors

Catherine M Rogers

Gavin E Morris

Toby WA Gould

Robert Bail

Sotiria Toumpaniari

Helen Harrington

JAMES DIXON JAMES.DIXON@NOTTINGHAM.AC.UK
Associate Professor

Kevin M Shakesheff

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FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering



Abstract

Electrospinning is a common technique used to fabricate fibrous scaffolds for tissue engineering applications. There is now growing interest in assessing the ability of collector plate design to influence the patterning of the fibres during the electrospinning process. In this study, we investigate a novel method to generate hybrid electrospun scaffolds consisting of both random fibres and a defined three-dimensional (3D) micro-topography at the surface, using patterned resin formers produced by rapid prototyping (RP). Poly(D,L-lactide-co-glycolide) was electrospun onto the engineered RP surfaces and the ability of these formers to influence microfibre patterning in the resulting scaffolds visualized by scanning electron microscopy. Electrospun scaffolds with patterns mirroring the microstructures of the formers were successfully fabricated. The effect of the resulting fibre patterns and 3D geometries on mammalian cell adhesion and proliferation was investigated by seeding enhanced green fluorescent protein labelled 3T3 fibroblasts onto the scaffolds. Following 24 h and four days of culture, the seeded scaffolds were visually assessed by confocal macro- and microscopy. The patterning of the fibres guided initial cell adhesion to the scaffold with subsequent proliferation over the geometry resulting in the cells being held in a 3D micro-topography. Such patterning could be designed to replicate a specific in vivo structure; we use the dermal papillae as an exemplar here. In conclusion, a novel, versatile and scalable method to produce hybrid electrospun scaffolds has been developed. The 3D directional cues of the patterned fibres have been shown to influence cell behaviour and could be used to culture cells within a similar 3D micro-topography as experienced in vivo.

Citation

Rogers, C. M., Morris, G. E., Gould, T. W., Bail, R., Toumpaniari, S., Harrington, H., …Rose, F. R. A. J. (2014). A novel technique for the production of electrospun scaffolds with tailored three-dimensional micro-patterns employing additive manufacturing. Biofabrication, 6(3), 035003. https://doi.org/10.1088/1758-5082/6/3/035003

Journal Article Type Article
Acceptance Date Mar 10, 2014
Online Publication Date Apr 11, 2014
Publication Date Apr 11, 2014
Deposit Date Mar 29, 2019
Publicly Available Date Mar 29, 2019
Journal Biofabrication
Print ISSN 1758-5082
Electronic ISSN 1758-5090
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 6
Issue 3
Pages 035003
DOI https://doi.org/10.1088/1758-5082/6/3/035003
Public URL https://nottingham-repository.worktribe.com/output/1712280
Publisher URL https://iopscience.iop.org/article/10.1088/1758-5082/6/3/035003
Contract Date Mar 29, 2019

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Publisher Licence URL
https://creativecommons.org/licenses/by/3.0/

Copyright Statement
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.





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