Skip to main content

Research Repository

Advanced Search

Mineralizing Coating on 3D Printed Scaffolds for the Promotion of Osseointegration

Hasan, Abshar; Bagnol, Romain; Owen, Robert; Latif, Arsalan; Rostam, Hassan M.; Elsharkawy, Sherif; Rose, Felicity R.A.J.; Rodríguez-Cabello, José Carlos; Ghaemmaghami, Amir M.; Eglin, David; Mata, Alvaro

Mineralizing Coating on 3D Printed Scaffolds for the Promotion of Osseointegration Thumbnail


Abshar Hasan

Romain Bagnol

Profile Image

Nottingham Research Fellow Fellowship

Arsalan Latif

Hassan M. Rostam

Sherif Elsharkawy

Profile Image

Professor of Biomaterials and Tissue Engineering

José Carlos Rodríguez-Cabello

David Eglin



Design and fabrication of implants that can perform better than autologous bone grafts remain an unmet challenge for the hard tissue regeneration in craniomaxillofacial applications. Here, we report an integrated approach combining additive manufacturing with supramolecular chemistry to develop acellular mineralizing 3D printed scaffolds for hard tissue regeneration. Our approach relies on an elastin-like recombinamer (ELR) coating designed to trigger and guide the growth of ordered apatite on the surface of 3D printed nylon scaffolds. Three test samples including a) uncoated nylon scaffolds (referred to as “Uncoated”), b) ELR coated scaffolds (referred to as “ELR only”), and c) ELR coated and in vitro mineralized scaffolds (referred to as “Pre-mineralized”) were prepared and tested for in vitro and in vivo performance. All test samples supported normal human immortalized mesenchymal stem cell adhesion, growth, and differentiation with enhanced cell proliferation observed in the “Pre-mineralized” samples. Using a rabbit calvarial in vivo model, ‘Pre-mineralized’ scaffolds also exhibited higher bone ingrowth into scaffold pores and cavities with higher tissue-implant integration. However, the coated scaffolds (“ELR only” and “Pre-mineralized”) did not exhibit significantly more new bone formation compared to “Uncoated” scaffolds. Overall, the mineralizing coating offers an opportunity to enhance integration of 3D printed bone implants. However, there is a need to further decipher and tune their immunologic response to develop truly osteoinductive/conductive surfaces.

Journal Article Type Article
Acceptance Date Apr 27, 2022
Online Publication Date Jun 27, 2022
Publication Date Jun 27, 2022
Deposit Date Jul 14, 2022
Publicly Available Date Jul 21, 2022
Journal Frontiers in Bioengineering and Biotechnology
Electronic ISSN 2296-4185
Publisher Frontiers Media SA
Peer Reviewed Peer Reviewed
Volume 10
Article Number 836386
Keywords Biomedical Engineering; Histology; Bioengineering; Biotechnology
Public URL
Publisher URL


You might also like

Downloadable Citations