Outputs (1327)

PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays (2024)
Journal Article
Stewart, C. L., Hook, A. L., Zelzer, M., Marlow, M., & Piccinini, A. M. (2024). PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays. Cell Biochemistry and Function, 42(5), Article e4097. https://doi.org/10.1002/cbf.4097

We identified that PLGA-PEG-PLGA hydrogels, which have been used in human clinical trials and possess a demonstrable safety profile, induced significant cytotoxicity in conventional in vitro assays. This major contradiction may lead to inconsistent a... Read More about PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays.

Impact of guidance change and financial incentivization on prescribing of sustainable inhaled medication in primary care (2024)
Presentation / Conference Contribution
Sonnex, K. (2024, May). Impact of guidance change and financial incentivization on prescribing of sustainable inhaled medication in primary care. Presented at PRIMM 34th Annual Scientific Meeting: Drug Utilisation: Learning from Practice & Research to Improve Patient Outcomes’, Manchester, UK

Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells (2024)
Journal Article
Comeo, E., Goulding, J., Lin, C.-Y., Groenen, M., Woolard, J., Kindon, N. D., Harwood, C. R., Platt, S., Briddon, S. J., Kilpatrick, L. E., Scammells, P. J., Hill, S. J., & Kellam, B. (2024). Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells. Journal of Medicinal Chemistry, https://doi.org/10.1021/acs.jmedchem.4c00835

The study of protein function and dynamics in their native cellular environment is essential for progressing fundamental science. To overcome the requirement of genetic modification of the protein or the limitations of dissociable fluorescent ligands... Read More about Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells.

Aptamer BT200 blocks interaction of K1405-1408 in the VWF-A1 domain with macrophage LRP1 (2024)
Journal Article
Chan Kwo Chion, A., Byrne, C., Atiq, F., Doherty, D., Aguila, S., Fazavana, J., Lopes, P., Karampini, E., Amin, A., Preston, R. J., Baker, R. I., McKinnon, T. A., Zhu, S., Gilbert, J. C., Emsley, J., Jilma, B., & O'Donnell, J. S. (in press). Aptamer BT200 blocks interaction of K1405-1408 in the VWF-A1 domain with macrophage LRP1. Blood, https://doi.org/10.1182/blood.2024024055

Rondaptivan pegol (previously BT200) is a PEGylated RNA aptamer that binds to the A1 domain of VWF. Recent clinical trials demonstrated that BT200 significantly increased plasma VWF-FVIII levels by attenuating VWF clearance. The biological mechanism(... Read More about Aptamer BT200 blocks interaction of K1405-1408 in the VWF-A1 domain with macrophage LRP1.

Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS (2024)
Journal Article
Lu, L., Kotowska, A. M., Kern, S., Fang, M., Rudd, T. R., Alexander, M. R., Scurr, D. J., & Zhu, Z. (2024). Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS. Analytical Chemistry, https://doi.org/10.1021/acs.analchem.4c01201

Growing clinical evidence reveals that systematic molecular alterations in the brain occur 20 years before the onset of AD pathological features. Apolipoprotein E4 (ApoE4) is one of the most significant genetic risk factors for Alzheimer’s disease (A... Read More about Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS.

β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3 (2024)
Journal Article
Owen, R., Wittkowske, C., Lacroix, D., Perrault, C. M., & Reilly, G. C. (2024). β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3. Connective Tissue Research, https://doi.org/10.1080/03008207.2024.2375065

Aim As osteoblasts deposit a mineralized collagen network, a subpopulation of these cells differentiates into osteocytes. Biochemical and mechanical stimuli, particularly fluid shear stress (FSS), are thought to regulate this, but their relative inf... Read More about β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3.

Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows (2024)
Journal Article
Cardoso, A. S., Whitby, A., Green, M. J., Kim, D.-H., & Randall, L. V. (2024). Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows. Animals, 14(14), Article 2030. https://doi.org/10.3390/ani14142030

The aim of this study was to identify with a high level of confidence metabolites previously identified as predictors of lameness and understand their biological relevance by carrying out pathway analyses. For the dairy cattle sector, lameness is a m... Read More about Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows.

Pilot and quantitative evaluation of the TARGET acne toolkit by UK pharmacy professionals working in general practice (2024)
Journal Article
Sonnex, K., Thornley, T., Fleming, N., Lakha, A., Lecky, D. M., Pillay, I., …Boyd, M. (2024). Pilot and quantitative evaluation of the TARGET acne toolkit by UK pharmacy professionals working in general practice. BMJ Open, 14(7), Article e081641. https://doi.org/10.1136/bmjopen-2023-081641

Background: Acne vulgaris (acne) is a common skin condition sometimes needing topical or oral antibiotic treatment. Pharmacists and pharmacy technicians (together known as pharmacy professionals) working in general practice are well placed to ensure... Read More about Pilot and quantitative evaluation of the TARGET acne toolkit by UK pharmacy professionals working in general practice.

Cellular and microenvironmental cues that promote macrophage fusion and foreign body response (2024)
Journal Article
Stewart, C. L., Marlow, M., Zelzer, M., Hook, A. L., & Piccinini, A. M. (in press). Cellular and microenvironmental cues that promote macrophage fusion and foreign body response. Frontiers in Immunology, 15, https://doi.org/10.3389/fimmu.2024.1411872

During the foreign body response (FBR), macrophages fuse to form foreign body giant cells (FBGCs). Modulation of FBGC formation can prevent biomaterial degradation and loss of therapeutic efficacy. However, the microenvironmental cues that dictate FB... Read More about Cellular and microenvironmental cues that promote macrophage fusion and foreign body response.

Discovery of MT-1207: A Novel, Potent Multitarget Inhibitor as a Promising Clinical Candidate for the Treatment of Hypertension (2024)
Journal Article
Wang, P., Zhu, H., Tian, J.-S., Zhu, W., Xu, S., Yao, H., Liu, J., Zhu, Z., Miao, C.-Y., & Xu, J. (2024). Discovery of MT-1207: A Novel, Potent Multitarget Inhibitor as a Promising Clinical Candidate for the Treatment of Hypertension. Journal of Medicinal Chemistry, https://doi.org/10.1021/acs.jmedchem.4c00626

Herein, a series of novel arylpiperazine (piperidine) derivatives were designed, synthesized, and evaluated for mechanisms of action through in vitro and in vivo studies. The most promising compound, II-13 (later named as MT-1207), is a potent α1 and... Read More about Discovery of MT-1207: A Novel, Potent Multitarget Inhibitor as a Promising Clinical Candidate for the Treatment of Hypertension.