Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

Magnusson, Johannes P.; Alexander, Cameron; Fern�ndez-Trillo, Francisco; Sicilia, Giovanna; Spain, Sebastian G.

Abstract

Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and ‘on–off’ switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition.

• Journal Article Type: Article
• Acceptance Date: Nov 4, 2013
• Online Publication Date: Feb 21, 2014
• Publication Date: Feb 21, 2014
• Deposit Date: May 31, 2018
• Publicly Available Date: Sep 9, 2019
• Journal: Nanoscale
• Print ISSN: 2040-3364
• Electronic ISSN: 2040-3372
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 4
• Pages: 2368-2374
• DOI: https://doi.org/10.1039/c3nr04952c
• Public URL: https://nottingham-repository.worktribe.com/output/1098176
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2014/NR/C3NR04952C#!divAbstract
• PMID: 00033079