All Outputs (36)

Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals (2016)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., Flynn, R. J., Martelli, F., Gosling, R. J., Davies, R. H., La Ragione, R. M., & Gough, K. C. (2016). Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6, Article 31186. https://doi.org/10.1038/srep31186

Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from va... Read More about Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals.

Mapping polyclonal antibody responses to bacterial infection using next generation phage display (2016)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., Tchorzewska, M., Martelli, F., Gosling, R. J., Davies, R. H., La Ragione, R. M., & Gough, K. C. (2016). Mapping polyclonal antibody responses to bacterial infection using next generation phage display. Scientific Reports, 6, Article 24232. https://doi.org/10.1038/srep24232

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next... Read More about Mapping polyclonal antibody responses to bacterial infection using next generation phage display.

Methods for differentiating prion types in food-producing animals (2015)
Journal Article
Gough, K. C., Rees, H. C., Ives, S. E., & Maddison, B. C. (2015). Methods for differentiating prion types in food-producing animals. Biology, 4(4), https://doi.org/10.3390/biology4040785

Prions are an enigma amongst infectious disease agents as they lack a genome yet confer specific pathologies thought to be dictated mainly, if not solely, by the conformation of the disease form of the prion protein (PrPSc). Prion diseases affect hum... Read More about Methods for differentiating prion types in food-producing animals.

Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission (2015)
Journal Article
Konold, T., Hawkins, S. A., Thurston, L. C., Maddison, B. C., Gough, K. C., Duarte, A., & Simmons, H. A. (2015). Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission. Frontiers in Veterinary Science, 2, Article 32. https://doi.org/10.3389/fvets.2015.00032

Classical scrapie is an environmentally transmissible prion disease of sheep and goats. Prions can persist and remain potentially infectious in the environment for many years and thus pose a risk of infecting animals after re-stocking. In vitro studi... Read More about Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission.

Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells (2015)
Journal Article
Gough, K. C., Maddison, B. C., Shikotra, A., Moiseeva, E. P., Yang, W., Jarvis, S., & Bradding, P. (2015). Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells. Respiratory Research, 16, Article 86. https://doi.org/10.1186/s12931-015-0245-z

Background: Human lung mast cells (HLMCs) infiltrate the airway epithelium and airway smooth muscle (ASM) in asthmatic airways. The mechanism of HLMC adhesion to both cell types is only partly defined, and adhesion is not inhibited by function-blocki... Read More about Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells.

Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals (2015)
Journal Article
Rees, H. C., Gough, K. C., Middleditch, D. J., Patmore, J. R., & Maddison, B. C. (2015). Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals. Journal of Applied Ecology, 52(4), https://doi.org/10.1111/1365-2664.12467

1. In Rees et al. (2014b), we reviewed the current status of environmental DNA (eDN A) tomonitor aquatic populations. Our aim was to focus on discus sion of methodologies used,application of eDNA analysis as a survey tool in ecology, and to include s... Read More about Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals.

Does the Presence of Scrapie Affect the Ability of Current Statutory Discriminatory Tests To Detect the Presence of Bovine Spongiform Encephalopathy? (2015)
Journal Article
Maddison, B. C., Gough, K. C., Bishop, K., Simmons, M. M., Chaplin, M. J., Vickery, C. M., Simon, S., Davis, L., Denyer, M., Lockey, R., Stack, M. J., O'Connor, M. J., Thorne, L., & Spiropoulos, J. (2015). Does the Presence of Scrapie Affect the Ability of Current Statutory Discriminatory Tests To Detect the Presence of Bovine Spongiform Encephalopathy?. Journal of Clinical Microbiology, 53(8), 2593-2604. https://doi.org/10.1128/jcm.00508-15

Current European Commission (EC) surveillance regulations require discriminatory testing of all transmissible spongiform encephalopathy (TSE)-positive small ruminant (SR) samples in order to classify them as bovine spongiform encephalopathy (BSE) or... Read More about Does the Presence of Scrapie Affect the Ability of Current Statutory Discriminatory Tests To Detect the Presence of Bovine Spongiform Encephalopathy?.

Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time (2015)
Journal Article
Maddison, B. C., Spiropoulos, J., Vickery, C. M., Lockey, R., Owen, J. P., Bishop, K., Baker, C. A., & Gough, K. C. (2015). Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time. Veterinary Research, 46, Article 46. https://doi.org/10.1186/s13567-015-0179-y

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation... Read More about Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time.

Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets (2015)
Journal Article
Spiliotopoulos, A., Owen, J. P., Maddison, B. C., Dreveny, I., Rees, H. C., & Gough, K. C. (2015). Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets. Journal of Immunological Methods, 420, https://doi.org/10.1016/j.jim.2015.03.005

Recently the analytical power of the latest high throughput next generation DNA sequencing platforms has been used to analyse phage that have been selected from the panning of large combinatorial libraries displaying either peptide or antibody ligand... Read More about Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets.

Circulation of prions within dust on a scrapie affected farm (2015)
Journal Article
Gough, K. C., Baker, C. A., Simmons, H. A., Hawkins, S. A., & Maddison, B. C. (2015). Circulation of prions within dust on a scrapie affected farm. Veterinary Research, 46, https://doi.org/10.1186/s13567-015-0176-1

Prion diseases are fatal neurological disorders that affect humans and animals. Scrapie of sheep/goats and Chronic Wasting Disease (CWD) of deer/elk are contagious prion diseases where environmental reservoirs have a direct link to the transmission o... Read More about Circulation of prions within dust on a scrapie affected farm.

Persistence of scrapie infectivity within a farm environment after cleaning and decontamination (2015)
Journal Article
Hawkins, S. A., Simmons, H. A., Gough, K. C., & Maddison, B. C. (2015). Persistence of scrapie infectivity within a farm environment after cleaning and decontamination. Veterinary Record, 176(4), https://doi.org/10.1136/vr.102743

Scrapie of sheep/goats and Chronic Wasting Disease (CWD) of deer/elk are contagious prion diseases where environmental reservoirs are directly implicated in the transmission of disease. In this study the effectiveness of recommended scrapie farm deco... Read More about Persistence of scrapie infectivity within a farm environment after cleaning and decontamination.

Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs (2014)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Gardner, D. S., Foster, N., Tchorzewska, M., La Ragione, R. M., & Gough, K. C. (2015). Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs. Animal Feed Science and Technology, 201, 57-65. https://doi.org/10.1016/j.anifeedsci.2014.12.005

Salmonellosis causes significant economic losses to the pig industry and contaminated pork products are an important source of Salmonella for humans. The EU ban on the use of antibiotic growth promoters in pig production, and the emergence of antibio... Read More about Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs.

The application of eDNA for monitoring of the great crested newt in the UK (2014)
Journal Article
Rees, H. C., Bishop, K., Middleditch, D. J., Patmore, J. R., Maddison, B. C., & Gough, K. C. (2014). The application of eDNA for monitoring of the great crested newt in the UK. Ecology and Evolution, 4(21), https://doi.org/10.1002/ece3.1272

Current ecological surveys for great crested newts are time-consuming and expensive and can only be carried out within a short survey window. Additional survey methods which would facilitate the detection of rare or protected species such as the grea... Read More about The application of eDNA for monitoring of the great crested newt in the UK.

Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification (2014)
Journal Article
Gough, K. C., Bishop, K., & Maddison, B. C. (2014). Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification. Journal of Clinical Microbiology, 52(11), https://doi.org/10.1128/JCM.01693-14

It is assumed that sheep and goats consumed the same bovine spongiform encephalopathy (BSE)-contaminated meat and bone meal that was fed to cattle and precipitated the BSE epidemic in the United Kingdom that peaked more than 20 years ago. De- spite i... Read More about Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification.

The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology (2014)
Journal Article
Rees, H. C., Maddison, B. C., Middleditch, D. J., Patmore, J. R., & Gough, K. C. (2014). The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51, https://doi.org/10.1111/1365-2664.12306

1. Knowledge of species distribution is critical to ecological management and conservation biology. Effective management requires the detection of populations, which can sometimes be at low densities and is usually based on visual detection and count... Read More about The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology.

Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines (2014)
Journal Article
Smith, C. M., Fry, S. C., Gough, K. C., Patel, A. J., Glenn, S., Goldrick, M., Roberts, I. S., Whitelam, G. C., & Andrew, P. W. (2014). Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines. PLoS ONE, 9(2), Article e88144. https://doi.org/10.1371/journal.pone.0088144

Bacterial polysaccharides have numerous clinical or industrial uses. Recombinant plants could offer the possibility of producing bacterial polysaccharides on a large scale and free of contaminating bacterial toxins and antigens. We investigated the f... Read More about Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines.