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Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines

Smith, Claire M.; Fry, Stephen C.; Gough, Kevin C.; Patel, Alexandra J.F.; Glenn, Sarah; Goldrick, Marie; Roberts, Ian S.; Whitelam, Garry C.; Andrew, Peter W.

Authors

Claire M. Smith

Stephen C. Fry

Kevin C. Gough

Alexandra J.F. Patel

Sarah Glenn

Marie Goldrick

Ian S. Roberts

Garry C. Whitelam

Peter W. Andrew



Abstract

Bacterial polysaccharides have numerous clinical or industrial uses. Recombinant plants could offer the possibility of producing bacterial polysaccharides on a large scale and free of contaminating bacterial toxins and antigens. We investigated the feasibility of this proposal by cloning and expressing the gene for the type 3 synthase (cps3S) of Streptococcus pneumoniae in Nicotinia tabacum, using the pCambia2301 vector and Agrobacterium tumefaciens-mediated gene transfer. In planta the recombinant synthase polymerised plant-derived UDP-glucose and UDP-glucuronic acid to form type 3 polysaccharide. Expression of the cps3S gene was detected by RT-PCR and production of the pneumococcal polysaccharide was detected in tobacco leaf extracts by double immunodiffusion, Western blotting and high-voltage paper electrophoresis. Because it is used a component of anti-pneumococcal vaccines, the immunogenicity of the plant-derived type 3 polysaccharide was tested. Mice immunised with extracts from recombinant plants were protected from challenge with a lethal dose of pneumococci in a model of pneumonia and the immunised mice had significantly elevated levels of serum anti-pneumococcal polysaccharide antibodies. This study provides the proof of the principle that bacterial polysaccharide can be successfully synthesised in plants and that these recombinant polysaccharides could be used as vaccines to protect against life-threatening infections.

Journal Article Type Article
Publication Date Feb 3, 2014
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 9
Issue 2
Article Number e88144
APA6 Citation Smith, C. M., Fry, S. C., Gough, K. C., Patel, A. J., Glenn, S., Goldrick, M., …Andrew, P. W. (2014). Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines. PLoS ONE, 9(2), doi:10.1371/journal.pone.0088144
DOI https://doi.org/10.1371/journal.pone.0088144
Publisher URL http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088144
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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