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Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals

Rees, Helen C.; Gough, Kevin C.; Middleditch, David J.; Patmore, James R.; Maddison, Ben C.

Authors

Helen C. Rees

Kevin C. Gough

David J. Middleditch

James R. Patmore

Ben C. Maddison



Abstract

1. In Rees et al. (2014b), we reviewed the current status of environmental DNA (eDN A) tomonitor aquatic populations. Our aim was to focus on discus sion of methodologies used,application of eDNA analysis as a survey tool in ecology, and to include some innovativeideas for using eDNA in conservation and management.
2. Roussel et al. (2015) claim that analysis of Rees et al. (2014b) and other publicationshighlights the downsides of the method, and they suggest that some conclusions should betoned down. Many of their arguments were covered in our original paper (Rees et al. ,2014b); however, they make the point that modelling approaches should be encouraged, andwe fully agree with this suggestion.
3. Roussel et al. (2015) also claim that we neglected to recognize that there are two sourcesof imperfect detection (at the field level and at the laboratory level). We feel that our reviewpaper implies this point.
4. Synthesis and applications. Roussel et al. (2015) reiterate many of the points made in theoriginal paper but do cover some additional areas that improve the debate on the use of envi-ronmental DNA (eDNA). Both the comment (Roussel et al., 2015) and our rebuttal clearlyhighlight that detailed laboratory protocols and rigorous field sampling design are crucial fac-tors which require sufficient reporting in the literature to allow for experimenta l comparisonand replication. Any development of a new method for eDNA detection should be compareddirectly with established ‘gold standard’ methods for the detection of the species or habitatunder investigation. None of the issues raised in Roussel et al. (2015) would alter our mainconclusions.

Journal Article Type Article
Publication Date Aug 15, 2015
Journal Journal of Applied Ecology
Print ISSN 0021-8901
Electronic ISSN 1365-2664
Publisher Wiley
Peer Reviewed Not Peer Reviewed
Volume 52
Issue 4
APA6 Citation Rees, H. C., Gough, K. C., Middleditch, D. J., Patmore, J. R., & Maddison, B. C. (2015). Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals. Journal of Applied Ecology, 52(4), doi:10.1111/1365-2664.12467
DOI https://doi.org/10.1111/1365-2664.12467
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/1365-2664.12467/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is the pre-peer reviewed version of the following article: Rees, H. C., Gough, K. C., Middleditch, D. J., Patmore, J. R. M., Maddison, B. C. (2015), Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals. Journal of Applied Ecology, 52: 827–831, which has been published in final form at http://onlinelibrary.wi...365-2664.12467/abstract ; doi: 10.1111/1365-2664.12467. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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