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The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology

Rees, Helen C.; Maddison, Ben C.; Middleditch, David J.; Patmore, James R.; Gough, Kevin C.

Authors

Helen C. Rees

Ben C. Maddison

David J. Middleditch

James R. Patmore

Kevin C. Gough



Abstract

1. Knowledge of species distribution is critical to ecological management and conservation biology. Effective management requires the detection of populations, which can sometimes be at low densities and is usually based on visual detection and counting.
2. Recently, there has been considerable interest in the detection of short species-specific environmental DNA (eDNA) fragments to allow aquatic species monitoring within different environments due to the potential of greater sensitivity over traditional survey methods which can be time-consuming and costly.
3. Environmental DNA analysis is increasingly being used in the detection of rare or invasive species and has also been applied to eDNA persistence studies and estimations of species biomass and distribution. When combined with next-generation sequencing methods, it has been demonstrated that entire faunas can be identified.
4. Different environments require different sampling methodologies, but there remain areas where laboratory methodologies could be standardized to allow results to be compared across studies.
5. Synthesis and applications. We review recently published studies that use eDNA to moni- tor aquatic populations, discuss the methodologies used and the application of eDNA analysis as a survey tool in ecology. We include innovative ideas for how eDNA can be used for conservation and management citing test cases, for instance, the potential for on-site analyses, including the application of eDNA analysis to carbon nanotube platforms or laser transmission spectroscopy to facilitate rapid on-site detections. The use of eDNA monitoring is already being adopted in the UK for ecological surveys.

Journal Article Type Article
Publication Date Jul 25, 2014
Journal Journal of Applied Ecology
Print ISSN 0021-8901
Electronic ISSN 0021-8901
Publisher Wiley
Peer Reviewed Not Peer Reviewed
Volume 51
APA6 Citation Rees, H. C., Maddison, B. C., Middleditch, D. J., Patmore, J. R., & Gough, K. C. (2014). The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51, doi:10.1111/1365-2664.12306
DOI https://doi.org/10.1111/1365-2664.12306
Keywords DNA Barcoding, Ecosystem Management, eDna, Invasive Species, Next-Generation Sequencing, PCR, Rare or Threatened Species, Species-Specific Detection, Water Sampling
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/1365-2664.12306/abstract;jsessionid=E26A5D7CC790A14A76A33D44C849A31A.f04t02
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is the pre-peer reviewed version of the following article: Rees, H.C., Maddison, B.C., Middleditch, D.J., Patmore, J.R.M., Gough, K.C. (2014), REVIEW: The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51: 1450–1459 doi: 10.1111/1365-2664.12306, which has been published in final form at http://onlinelibrary.wi...65-2664.12306/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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