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Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time

Maddison, Ben C.; Spiropoulos, John; Vickery, Christopher M.; Lockey, Ricahrd; Owen, Jonathan P.; Bishop, Keith; Baker, Claire A.; Gough, Kevin C.

Authors

Ben C. Maddison

John Spiropoulos

Christopher M. Vickery

Ricahrd Lockey

Jonathan P. Owen

Keith Bishop

Claire A. Baker

Kevin C. Gough



Abstract

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrPSc phenotypes were present: G338 or Apl338ii. Thirteen months later some divergent PrPSc phenotypes were seen: a mixture of Apl338ii with either G338 or P338, and a completely novel PrPSc deposition, designated Cag338. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrPSc biological/biochemical properties.

Journal Article Type Article
Publication Date May 1, 2015
Journal Veterinary Research
Print ISSN 0928-4249
Electronic ISSN 0928-4249
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 46
Article Number 46
APA6 Citation Maddison, B. C., Spiropoulos, J., Vickery, C. M., Lockey, R., Owen, J. P., Bishop, K., …Gough, K. C. (2015). Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time. Veterinary Research, 46, doi:10.1186/s13567-015-0179-y
DOI https://doi.org/10.1186/s13567-015-0179-y
Publisher URL http://www.veterinaryresearch.org/content/46/1/46
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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