Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time

Maddison, Ben C.; Spiropoulos, John; Vickery, Christopher M.; Lockey, Ricahrd; Owen, Jonathan P.; Bishop, Keith; Baker, Claire A.; Gough, Kevin C.

Abstract

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrPSc phenotypes were present: G338 or Apl338ii. Thirteen months later some divergent PrPSc phenotypes were seen: a mixture of Apl338ii with either G338 or P338, and a completely novel PrPSc deposition, designated Cag338. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrPSc biological/biochemical properties.

Citation

Maddison, B. C., Spiropoulos, J., Vickery, C. M., Lockey, R., Owen, J. P., Bishop, K., Baker, C. A., & Gough, K. C. (2015). Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time. Veterinary Research, 46, Article 46. https://doi.org/10.1186/s13567-015-0179-y

• Journal Article Type: Article
• Publication Date: May 1, 2015
• Deposit Date: Sep 24, 2015
• Publicly Available Date: Sep 24, 2015
• Journal: Veterinary Research
• Print ISSN: 0928-4249
• Electronic ISSN: 1297-9716
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 46
• Article Number: 46
• DOI: https://doi.org/10.1186/s13567-015-0179-y
• Public URL: https://nottingham-repository.worktribe.com/output/748547
• Publisher URL: http://www.veterinaryresearch.org/content/46/1/46