Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time

Maddison, Ben C.; Spiropoulos, John; Vickery, Christopher M.; Lockey, Ricahrd; Owen, Jonathan P.; Bishop, Keith; Baker, Claire A.; Gough, Kevin C.

Abstract

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrPSc phenotypes were present: G338 or Apl338ii. Thirteen months later some divergent PrPSc phenotypes were seen: a mixture of Apl338ii with either G338 or P338, and a completely novel PrPSc deposition, designated Cag338. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrPSc biological/biochemical properties.

Citation

Maddison, B. C., Spiropoulos, J., Vickery, C. M., Lockey, R., Owen, J. P., Bishop, K., …Gough, K. C. (2015). Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time. Veterinary Research, 46, Article 46. https://doi.org/10.1186/s13567-015-0179-y

• Journal Article Type: Article
• Publication Date: May 1, 2015
• Deposit Date: Sep 24, 2015
• Publicly Available Date: Sep 24, 2015
• Journal: Veterinary Research
• Print ISSN: 0928-4249
• Electronic ISSN: 0928-4249
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 46
• Article Number: 46
• DOI: https://doi.org/10.1186/s13567-015-0179-y
• Public URL: https://nottingham-repository.worktribe.com/output/748547
• Publisher URL: http://www.veterinaryresearch.org/content/46/1/46