All Outputs (65)

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers (2010)
Journal Article

Protein self-organization is essential for the establishment and maintenance of nuclear architecture and for the regulation of gene expression. We have shown previously that the Proline-Rich Homeodomain protein (PRH/Hex) self-assembles to form oligom... Read More about DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.

In situ subcellular fractionation of adherent and non-adherent mammalian cells (2010)
Journal Article

Protein function is intimately coupled to protein localization. Although some proteins are restricted to a specific location or subcellular compartment, many proteins are present as a freely diffusing population in free exchange with a sub-population... Read More about In situ subcellular fractionation of adherent and non-adherent mammalian cells.

PRH/Hex controls cell survival through coordinate transcriptional regulation of vascular endothelial growth factor signaling (2010)
Journal Article

The proline-rich homeodomain protein (PRH) plays multiple roles in the control of gene expression during embryonic development and in the adult. Vascular endothelial growth factor (VEGF) is a mitogen that stimulates cell proliferation and survival vi... Read More about PRH/Hex controls cell survival through coordinate transcriptional regulation of vascular endothelial growth factor signaling.

The human papillomavirus E7-E2 interaction mechanism in vitro reveals a finely turned system for modulating available E7 and E2 proteins (2009)
Journal Article

Transcription of the human papillomavirus E7 oncoprotein is negatively controlled by the viral E2 protein, and loss of this repression leads to irreversible transformation and carcinogenesis. Here we show that interaction of the HPV16 E7 protein with... Read More about The human papillomavirus E7-E2 interaction mechanism in vitro reveals a finely turned system for modulating available E7 and E2 proteins.

CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding (2009)
Journal Article

The proline-rich homeodomain protein (PRH/Hex) regulates transcription by binding to specific DNA sequences and regulates mRNA transport by binding to translation initiation factor eIF4E. Protein kinase CK2 plays multiple roles in the regulation of g... Read More about CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding.

The regulation of cell proliferation by the papillomavirus early proteins (2009)
Journal Article

The human papillomavirus (HPV) E6 and E7 oncogenes have direct effects on host cell proliferation. The viral E2 protein regulates transcription of E6 and E7 and thereby has an indirect effect on cell proliferation. In HPV-induced tumours, misappropri... Read More about The regulation of cell proliferation by the papillomavirus early proteins.

The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors (2008)
Journal Article

The PRH (proline-rich homeodomain) [also known as Hex (haematopoietically expressed homeobox)] protein is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haemat... Read More about The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors.

DNA wrapping and distortion by an oligomeric homeodomain protein (2008)
Journal Article

Many transcription factors alter DNA or chromatin structure. Changes in chromatin structure are often brought about by the recruitment of chromatin-binding proteins, chromatin-modifying proteins, or other transcription co-activator or co-repressor pr... Read More about DNA wrapping and distortion by an oligomeric homeodomain protein.

The papillomavirus E2 DNA binding domain (2008)
Journal Article

The DNA binding domain of the E2 master regulator from papillomaviruses is the primary effector for most the essential activities controlled by this protein. In this review we focus on the properties of the DNA binding domain of human papillomavirus... Read More about The papillomavirus E2 DNA binding domain.

p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein (2008)
Journal Article

Background. Human papillomavirus (HPV) DNA replication can be inhibited by the cellular tumour suppressor protein p53. However, the mechanism through which p53 inhibits viral replication and the role that this might play in the HPV life cycle are not... Read More about p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein.

Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics (2007)
Journal Article

Mucosal human papillomaviruses (HPVs) are etiological agents of oral, anal and genital cancer. Properties of high- and low-risk HPV types cannot be reduced to discrete molecular traits. The E2 protein regulates viral replication and transcription thr... Read More about Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics.

A cancer cell-specific inducer of apoptosis (2007)
Journal Article

Human papillomavirus (HPV) DNA is found in virtually all cervical cancers, strongly suggesting that these viruses are necessary to initiate this disease. The HPV E2 protein is required for viral replication, but E2 expression is usually lost in HPV-t... Read More about A cancer cell-specific inducer of apoptosis.

Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy (2007)
Journal Article

Galanin expression markedly increases in the dorsal root ganglion (DRG) after sciatic nerve axotomy and modulates pain behavior and regeneration of sensory neurons. Here, we describe transgenic mice expressing constructs with varying amounts of seque... Read More about Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy.

A quadrivalent vaccine for human papillomavirus (2006)
Journal Article

Cervical cancer is responsible for at least 13,500 deaths a year in the European Union and more than 30 times that worldwide. DNA from a group of human papillomaviruses (HPVs) is found in virtually all cervical carcinoma cells, and these so-called hi... Read More about A quadrivalent vaccine for human papillomavirus.

The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein (2006)
Journal Article

The E2 proteins are transcription/replication factors from papillomaviruses. Human papillomaviruses (HPVs) can be broadly divided in two groups; low-risk HPV subtypes cause benign warts while high-risk HPVs give rise to cervical cancer. Although a ra... Read More about The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein.

Development of a topical protein therapeutic for human papillomavirus and associated cancers (2006)
Journal Article

E2 proteins from high- and low-risk human papillomavirus types differ in their ability to bind p53 and induce apoptotic cell death (2006)
Journal Article

Oligomerisation of the developmental regulator proline rich homeodomain (PRH/Hex) is mediated by a novel proline-rich dimerisation domain (2006)
Journal Article

Homeodomain proteins regulate multiple developmental pathways by altering gene expression temporally and in a tissue-specific fashion. The Proline Rich Homeodomain protein (PRH/Hex) is a transcription factor and an essential regulator of embryonic de... Read More about Oligomerisation of the developmental regulator proline rich homeodomain (PRH/Hex) is mediated by a novel proline-rich dimerisation domain.

Purification and characterisation of the PRH homeodomain: removal of the N-terminal domain of PRH increases the PRH homeodomain-DNA interaction (2006)
Journal Article

The Proline-Rich Homeodomain (PRH) protein is a regulator of transcription and translation and plays a key role in the control of cell proliferation and cell differentiation. PRH contains an N-terminal proline-rich domain that can repress transcripti... Read More about Purification and characterisation of the PRH homeodomain: removal of the N-terminal domain of PRH increases the PRH homeodomain-DNA interaction.

Diffusible VP22-E2 protein kills bystander cells and offers a route for cervical cancer gene therapy (2006)
Journal Article

Human papillomaviruses (HPVs) are a causative agent of cervical cancer and are implicated in several other types of malignant disease including cancer of the vulva, oral cancer, and skin cancer. In HPV-transformed cells, expression of the viral E6 an... Read More about Diffusible VP22-E2 protein kills bystander cells and offers a route for cervical cancer gene therapy.