Cecile Desjobert
The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors
Desjobert, Cecile; Noy, Peter; Swingler, Tracey; Williams, Hannah; Gaston, Kevin; Jayaraman, Padma-Sheela
Authors
Peter Noy
Tracey Swingler
Hannah Williams
Professor KEVIN GASTON Kevin.Gaston@nottingham.ac.uk
Professor of Cancer Studies
Padma-Sheela Jayaraman
Abstract
The PRH (proline-rich homeodomain) [also known as Hex (haematopoietically expressed homeobox)] protein is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. The Groucho/TLE (transducin-like enhancer) family of co-repressor proteins also regulate development and modulate the activity of many DNA-binding transcription factors during a range of diverse cellular processes including haematopoiesis. We have shown previously that PRH is a repressor of transcription in haematopoietic cells and that an Eh-1 (Engrailed homology) motif present within the N-terminal transcription repression domain of PRH mediates binding to Groucho/TLE proteins and enables co-repression. In the present study we demonstrate that PRH regulates the nuclear retention of TLE proteins during cellular fractionation. We show that transcriptional repression and the nuclear retention of TLE proteins requires PRH to bind to both TLE and DNA. In addition, we characterize a trans-dominant-negative PRH protein that inhibits wild-type PRH activity by sequestering TLE proteins to specific subnuclear domains. These results demonstrate that transcriptional repression by PRH is dependent on TLE availability and suggest that subnuclear localization of TLE plays an important role in transcriptional repression by PRH.
Citation
Desjobert, C., Noy, P., Swingler, T., Williams, H., Gaston, K., & Jayaraman, P. (2009). The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors. BBA - Biomembranes, 417(1), 121-132. doi:10.1042/BJ20080872
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 19, 2008 |
Online Publication Date | Aug 19, 2008 |
Publication Date | 2009-01 |
Deposit Date | Nov 1, 2018 |
Publicly Available Date | Mar 28, 2024 |
Journal | Biochimica et Biophysica Acta (BBA) - Biomembranes |
Print ISSN | 0005-2736 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 417 |
Issue | 1 |
Pages | 121-132 |
DOI | https://doi.org/10.1042/BJ20080872 |
Public URL | https://nottingham-repository.worktribe.com/output/1037685 |
Publisher URL | http://www.biochemj.org/content/417/1/121 |
Files
121.full
(723 Kb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by-nc/3.0/
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