All Outputs (95)

IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection (2019)
Journal Article

While adaptive immune responses against hepatitis C virus (HCV) infection have been studied in great detail, the role of innate immunity in protection against HCV infection and immune evasion is only partially understood. Interferon‐induced transmemb... Read More about IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.

Cross-genotype AR3-specific neutralizing antibodies confer long-term protection in injecting drug users after HCV clearance (2019)
Journal Article

Background & Aims Understanding immune protection against hepatitis C virus (HCV) infection is necessary for designing an effective vaccine. A number of broadly-reactive, neutralizing antibodies have been isolated from B cells of HCV-infected subjec... Read More about Cross-genotype AR3-specific neutralizing antibodies confer long-term protection in injecting drug users after HCV clearance.

Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses (2019)
Journal Article

The liver-expressed pattern recognition receptors (PRRs) mannose binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize... Read More about Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses.

Shared common ancestry of rodent alphacoronaviruses sampled globally (2019)
Journal Article

The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of r... Read More about Shared common ancestry of rodent alphacoronaviruses sampled globally.

Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells (2019)
Journal Article

Development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge. Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance. However, a signif... Read More about Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells.

InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones (2018)
Book Chapter

This chapter describes how to generate chimeric molecular cassettes that are ready to receive PCR-amplified E1/E2 genes using new DNA cloning technology. The method is divided into three sections: (1) generation of a ΔCore-NS2 cassette based upon the... Read More about InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones.

Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins (2018)
Book Chapter

Experimental characterization of the properties of authentic viruses circulating in infected individuals presents a problem when investigating RNA viruses with error-prone polymerases. The hepatitis C virus provides an extreme example of RNA virus ge... Read More about Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins.

Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp) (2018)
Book Chapter

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface... Read More about Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp).

Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies (2018)
Journal Article

Global eradication of hepatitis C virus (HCV) infection will require an efficacious vaccine capable of eliciting protective immunity against genetically diverse HCV strains. Natural spontaneous resolution of HCV infection is associated with productio... Read More about Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies.

Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 (2017)
Journal Article

Monoclonal antibodies (mAbs) targeting the hepatitis C virus (HCV) envelope have been raised mainly against envelope protein 2 (E2), while the antigenic epitopes of envelope protein 1 (E1) are not fully identified. Here we describe the detailed chara... Read More about Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1.

A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo (2017)
Journal Article

Infections with hepatitis C virus (HCV) represent a worldwide health burden and a prophylactic vaccine is still not available. Liver transplantation (LT) is often the only option for patients with HCV-induced end-stage liver disease. However, immedia... Read More about A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo.

Human Adaptation of Ebola Virus during the West African Outbreak (2016)
Journal Article

The 2013–2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the cocircul... Read More about Human Adaptation of Ebola Virus during the West African Outbreak.

Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin (2016)
Journal Article

Hepatitis C virus (HCV)-induced end-stage liver disease is the major indication for liver transplantation (LT). However, reinfection of the liver graft is still common, especially in patients with detectable viral load at the time of LT. Limited data... Read More about Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin.

Novel functional hepatitis C virus glycoprotein isolates identified using an optimized viral pseudotype entry assay (2016)
Journal Article

Retrovirus pseudotypes are a highly tractable model used to study the entry pathways of enveloped viruses. This model has been extensively applied to the study of the hepatitis C virus (HCV) entry pathway, pre-clinical screening of antiviral antibodi... Read More about Novel functional hepatitis C virus glycoprotein isolates identified using an optimized viral pseudotype entry assay.

Flexible and rapid construction of viral chimeras applied to hepatitis C virus (2016)
Journal Article

A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flex... Read More about Flexible and rapid construction of viral chimeras applied to hepatitis C virus.

Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals (2016)
Journal Article

HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequen... Read More about Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals.

Discovery of novel alphacoronaviruses in European rodents and shrews (2016)
Journal Article

Eight hundred and thirteen European rodents and shrews encompassing seven different species were screened for alphacoronaviruses using PCR detection. Novel alphacoronaviruses were detected in the species Rattus norvegicus, Microtus agrestis, Sorex ar... Read More about Discovery of novel alphacoronaviruses in European rodents and shrews.

Novel human anti-claudin 1 mAbs inhibit hepatitis C virus infection and may synergize with anti-SRB1 mAb (2016)
Journal Article

Hepatitis C virus (HCV) is a major cause of chronic hepatitis and liver carcinoma and new therapies based on novel targets are needed. The tight junction protein claudin 1 (CLDN-1) is essential for HCV cell entry and spread, and anti-CLDN-1 rat and m... Read More about Novel human anti-claudin 1 mAbs inhibit hepatitis C virus infection and may synergize with anti-SRB1 mAb.

A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance (2015)
Journal Article

Despite significant advances in the treatment of hepatitis C virus (HCV) infection, the need to develop preventative vaccines remains. Identification of the best vaccine candidates and evaluation of their performance in preclinical and clinical devel... Read More about A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance.

An ancestral host defence peptide within human beta-defensin 3 recapitulates the antibacterial and antiviral activity of the full-length molecule (2015)
Journal Article

Host defence peptides (HDPs) are critical components of innate immunity. Despite their diversity, they share common features including a structural signature, designated “γ-core motif”. We reasoned that for each HDPs evolved from an ancestral γ-core,... Read More about An ancestral host defence peptide within human beta-defensin 3 recapitulates the antibacterial and antiviral activity of the full-length molecule.