Justin R. Bailey
Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)
Bailey, Justin R.; Urbanowicz, Richard A.; Ball, Jonathan K.; Law, Mansun; Foung, Steven K. H.
Richard A. Urbanowicz
Jonathan K. Ball
Steven K. H. Foung
Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.
|Publication Date||Jan 1, 2019|
|Journal||Methods in Molecular Biology; Hepatitis C Virus Protocols|
|Series Title||Methods in Molecular Biology|
|Book Title||Hepatitis C Virus Protocols|
|APA6 Citation||Bailey, J. R., Urbanowicz, R. A., Ball, J. K., Law, M., & Foung, S. K. H. (2019). Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp). In M. Law (Ed.), Hepatitis C Virus Protocols, 441-450. Springer. doi:10.1007/978-1-4939-8976-8_30|
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