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Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)

Bailey, Justin R.; Urbanowicz, Richard A.; Ball, Jonathan K.; Law, Mansun; Foung, Steven K. H.

Authors

Justin R. Bailey

Richard A. Urbanowicz

Jonathan K. Ball

Mansun Law

Steven K. H. Foung



Contributors

Mansun Law
Editor

Abstract

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.

Publication Date Jan 1, 2019
Journal Methods in Molecular Biology; Hepatitis C Virus Protocols
Electronic ISSN 1940-6029
Publisher Springer
Pages 441-450
Series Title Methods in Molecular Biology
Series Number 1911
Book Title Hepatitis C Virus Protocols
Chapter Number 30
ISBN 9781493989751
APA6 Citation Bailey, J. R., Urbanowicz, R. A., Ball, J. K., Law, M., & Foung, S. K. H. (2019). Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp). In M. Law (Ed.), Hepatitis C Virus Protocols, 441-450. Springer. doi:10.1007/978-1-4939-8976-8_30
DOI https://doi.org/10.1007/978-1-4939-8976-8_30
Publisher URL https://link.springer.com/protocol/10.1007%2F978-1-4939-8976-8_30
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