THEOCHARIS TSOLERIDIS T.Tsoleridis@nottingham.ac.uk
Research Fellow
Shared common ancestry of rodent alphacoronaviruses sampled globally
Tsoleridis, Theocharis; Chappell, Joseph G; Onianwa, Okechukwu; Marston, Denise A; Fooks, Anthony R; Monchatreāleroy, Elodie; Umhang, Gérald; Müller, Marcel A; Drexler, Jan F; Drosten, Christian; Tarlinton, Rachael E; Mcclure, Charles P; Holmes, Edward C; K 7 Ball, Jonathan
Authors
Joseph G Chappell
Okechukwu Onianwa
Denise A Marston
Anthony R Fooks
Elodie Monchatreāleroy
Gérald Umhang
Marcel A Müller
Jan F Drexler
Christian Drosten
Professor RACHAEL TARLINTON rachael.tarlinton@nottingham.ac.uk
Associate Professor
Charles P Mcclure
Edward C Holmes
JONATHAN BALL jonathan.ball@nottingham.ac.uk
Professor of Molecular Virology
Abstract
The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of rodent CoVs in more detail, particularly the relative frequencies of virus-host co-divergence and cross-species transmission, we recovered longer fragments of CoV genomes from previously discovered European rodent alpha-CoVs using a combination of PCR and high-throughput sequencing. Accordingly, the full genome sequence was retrieved from the UK rat coronavirus, along with partial genome sequences from the UK field vole and Poland-resident bank vole CoVs, and a short conserved ORF1b fragment from the French rabbit CoV. Genome and phylogenetic analysis showed that despite their diverse geographic origins, all rodent alpha-CoVs formed a single monophyletic group and shared similar features such as the same gene constellations, a recombinant beta-CoV spike gene, and similar core transcriptional regulatory sequences (TRS). These data suggest that all rodent alpha CoVs sampled so far originate from a single common ancestor, and that there has likely been a long-term association between alpha CoVs and rodents. Despite this likely antiquity, the phylogenetic pattern of the alpha-CoVs was also suggestive of relatively frequent host-jumping among the different rodent species.
Citation
Tsoleridis, T., Chappell, J. G., Onianwa, O., Marston, D. A., Fooks, A. R., Monchatreāleroy, E., …K 7 Ball, J. (2019). Shared common ancestry of rodent alphacoronaviruses sampled globally. Viruses, 11(2), Article 125. https://doi.org/10.3390/v11020125
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 28, 2019 |
Online Publication Date | Jan 30, 2019 |
Publication Date | Jan 30, 2019 |
Deposit Date | Jan 29, 2019 |
Publicly Available Date | Jan 29, 2019 |
Journal | Viruses |
Electronic ISSN | 1999-4915 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 2 |
Article Number | 125 |
DOI | https://doi.org/10.3390/v11020125 |
Keywords | coronavirus; alphacoronavirus; rodents; ancestry; recombination; evolution |
Public URL | https://nottingham-repository.worktribe.com/output/1502960 |
Publisher URL | https://www.mdpi.com/1999-4915/11/2/125/htm |
Files
Viruses-436134
(3.7 Mb)
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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