All Outputs (20)

Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement (2020)
Journal Article

©2020 American Association for Cancer Research. Murine IgG3 glycan-targeting mAb often induces direct cell killing in the absence of immune effector cells or complement via a proinflammatory mechanism resembling oncotic necrosis. This cancer cell kil... Read More about Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement.

Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners (2018)
Journal Article

Many subsurface microorganisms couple their metabolism to the reduction or oxidation of extracellular substrates. For example, anaerobic mineral-respiring bacteria can use external metal oxides as terminal electron acceptors during respiration. Porin... Read More about Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners.

Reconstructing promoter activity from Lux bioluminescent reporters (2017)
Journal Article

The bacterial Lux system is used as a gene expression reporter. It is fast, sensitive and non-destructive, enabling high frequency measurements. Originally developed for bacterial cells, it has also been adapted for eukaryotic cells, and can be used... Read More about Reconstructing promoter activity from Lux bioluminescent reporters.

Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA (2017)
Journal Article

© 2017 The Author(s). The ParB protein, KorB, from the RK2 plasmid is required for DNA partitioning and transcriptional repression. It acts co-operatively with other proteins, including the repressor KorA. Like many multifunctional proteins, KorB con... Read More about Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA.

Accounting for thermodynamic non-ideality in the Guinier region of small-angle scattering data of proteins (2016)
Journal Article

Hydrodynamic studies of the solution properties of proteins and other biological macromolecules are often hard to interpret when the sample is present at a reasonably concentrated solution. The reason for this is that solutions exhibit deviations fro... Read More about Accounting for thermodynamic non-ideality in the Guinier region of small-angle scattering data of proteins.

STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways (2016)
Journal Article

STAT2 is the quintessential transcription factor for type 1 interferons (IFNs), where it functions as a heterodimer with STAT1. However, the human and murine STAT2-deficient phenotypes suggest important additional and currently unidentified type 1 IF... Read More about STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways.

SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance (2016)
Journal Article

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an... Read More about SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance.

Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator (2016)
Journal Article

The IncP (Incompatibility group P) plasmids are important carriers in the spread of antibiotic resistance across Gram-negative bacteria. Gene expression in the IncP-1 plasmids is stringently controlled by a network of four global repressors, KorA, Ko... Read More about Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.

Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction (2015)
Journal Article

The bacterial single-stranded DNA (ssDNA) binding protein SSB is a strictly conserved and essential protein involved in diverse functions of DNA metabolism, including replication and repair. SSB comprises a well-characterized tetrameric core of N-ter... Read More about Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction.

Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1 (2015)
Journal Article

Human Carboxylesterase 1 (hCES1) is the key liver microsomal enzyme responsible for detoxification and metabolism of a variety of clinical drugs. To analyse the role of the single N-linked glycan on the structure and activity of the enzyme, authentic... Read More about Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1.

Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions (2015)
Journal Article

This investigation examined the feasibility of manipulating the rotor speed in sedimentation velocity experiments to spontaneously generate an approximate steady-state condition where the extent of diffusional spreading is matched exactly by the boun... Read More about Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions.

Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport (2015)
Journal Article

Mammals obtain nitrogen via the uptake of di- and tri-peptides in the gastrointestinal tract through the action of PepT1 and PepT2, which are members of the POT family of proton-coupled oligopeptide transporters. PepT1 and PepT2 also play an importan... Read More about Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport.

A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation (2015)
Journal Article

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose o... Read More about A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation.

Concentration dependence of translational diffusion coefficients for globular proteins (2014)
Journal Article

This investigation examines published results of traditional diffusion experiments on ovalbumin and bovine serum albumin to determine the extent to which assumed concentration independence of the translational diffusion coefficient is a reasonable ap... Read More about Concentration dependence of translational diffusion coefficients for globular proteins.

N-terminal Domain of Prion Protein Directs Its Oligomeric Association (2014)
Journal Article

The self-association of prion protein (PrP) is a critical step in the pathology of prion diseases. It is increasingly recognized that small non-fibrillar ?-sheet-rich oligomers of PrP may be of crucial importance in the prion disease process. Here, w... Read More about N-terminal Domain of Prion Protein Directs Its Oligomeric Association.

Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus (2014)
Journal Article

Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be u... Read More about Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

Structure and Catalytic Regulatory Function of Ubiquitin Specific Protease 11 N-Terminal and Ubiquitin-like Domains (2014)
Journal Article

The ubiquitin specific protease 11 (USP11) is implicated in DNA repair, viral RNA replication, and TGF? signaling. We report the first characterization of the USP11 domain architecture and its role in regulating the enzymatic activity. USP11 consists... Read More about Structure and Catalytic Regulatory Function of Ubiquitin Specific Protease 11 N-Terminal and Ubiquitin-like Domains.

Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution (2014)
Journal Article

Anomalous small angle X-ray scattering can in principle be used to determine distances between metal label species on biological molecules. Previous experimental studies in the past were unable to distinguish the label-label scattering contribution f... Read More about Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution.

Synthesis of ?-glucan in mycobacteria involves a hetero-octameric complex of trehalose synthase TreS and Maltokinase Pep2 (2013)
Journal Article

Recent evidence established that the cell envelope of Mycobacterium tuberculosis, the bacillus causing tuberculosis (TB), is coated by an ?-glucan-containing capsule that has been implicated in persistence in a mouse infection model. As one of three... Read More about Synthesis of ?-glucan in mycobacteria involves a hetero-octameric complex of trehalose synthase TreS and Maltokinase Pep2.

Crystal structure of signal regulatory protein gamma (SIRP?) in complex with an antibody Fab fragment (2013)
Journal Article

BACKGROUND Signal Regulatory Protein γ (SIRPγ) is a member of a closely related family of three cell surface receptors implicated in modulating immune/inflammatory responses. SIRPγ is expressed on T lymphocytes where it appears to be involved in t... Read More about Crystal structure of signal regulatory protein gamma (SIRP?) in complex with an antibody Fab fragment.