Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport

Beale, John H.; Parker, Joanne L.; Samsudin, Firdaus; Barrett, Anne L.; Senan, Anish; Bird, Louise E.; Scott, David; Owens, Raymond J.; Sansom, Mark S.P.; Tucker, Stephen J.; Meredith, David; Fowler, Philip W.; Newstead, Simon

Abstract

Mammals obtain nitrogen via the uptake of di- and tri-peptides in the gastrointestinal tract through the action of PepT1 and PepT2, which are members of the POT family of proton-coupled oligopeptide transporters. PepT1 and PepT2 also play an important role in drug transport in the human body. Recent crystal structures of bacterial homologs revealed a conserved peptide-binding site and mechanism of transport. However, a key structural difference exists between bacterial and mammalian homologs with only the latter containing a large extracellular domain, the function of which is currently unknown. Here, we present the crystal structure of the extracellular domain from both PepT1 and PepT2 that reveal two immunoglobulin-like folds connected in tandem, providing structural insight into mammalian peptide transport. Functional and biophysical studies demonstrate that these domains interact with the intestinal protease trypsin, suggesting a role in clustering proteolytic activity to the site of peptide transport in eukaryotic cells.

Citation

Beale, J. H., Parker, J. L., Samsudin, F., Barrett, A. L., Senan, A., Bird, L. E., …Newstead, S. (2015). Crystal structures of the extracellular domain from PepT1 and PepT2 provide novel insights into mammalian pPeptide transport. Structure, 23(10), https://doi.org/10.1016/j.str.2015.07.016

• Journal Article Type: Article
• Acceptance Date: Jul 28, 2015
• Online Publication Date: Aug 27, 2015
• Publication Date: Oct 6, 2015
• Deposit Date: Apr 20, 2017
• Publicly Available Date: Apr 20, 2017
• Journal: Structure (London, England : 1993)
• Print ISSN: 0969-2126
• Electronic ISSN: 1878-4186
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Issue: 10
• DOI: https://doi.org/10.1016/j.str.2015.07.016
• Public URL: https://nottingham-repository.worktribe.com/output/764469
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0969212615003226
• Contract Date: Apr 20, 2017