Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution

Pinfield, Valerie J.; Scott, David J.

Abstract

Anomalous small angle X-ray scattering can in principle be used to determine distances between metal label species on biological molecules. Previous experimental studies in the past were unable to distinguish the label-label scattering contribution from that of the molecule, because of the use of atomic labels; these labels contribute only a small proportion of the total scattering signal. However, with the development of nanocrystal labels (of 50-100 atoms) there is the possibility for a renewed attempt at applying anomalous small angle X-ray scattering for distance measurement. This is because the contribution to the scattered signal is necessarily considerably stronger than for atomic labels. Here we demonstrate through simulations, the feasibility of the technique to determine the end-to-end distances of labelled nucleic acid molecules as well as other internal distances mimicking a labelled DNA binding protein if the labels are dissimilar metal nanocrystals. Of crucial importance is the ratio of mass of the nanocrystals to that of the labelled macromolecule, as well as the level of statistical errors in the scattering intensity measurements. The mathematics behind the distance determination process is presented, along with a fitting routine than incorporates maximum entropy regularisation.

Citation

Pinfield, V. J., & Scott, D. J. (2014). Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution. PLoS ONE, 9(4), Article e95664. https://doi.org/10.1371/journal.pone.0095664

• Journal Article Type: Article
• Acceptance Date: Mar 29, 2014
• Online Publication Date: Apr 23, 2014
• Publication Date: Apr 23, 2014
• Deposit Date: Apr 20, 2017
• Publicly Available Date: Apr 20, 2017
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 4
• Article Number: e95664
• DOI: https://doi.org/10.1371/journal.pone.0095664
• Public URL: https://nottingham-repository.worktribe.com/output/726664
• Publisher URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095664
• Additional Information: Pinfield VJ, Scott DJ (2014) Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution. PLoS ONE 9(4): e95664. doi:10.1371/journal.pone.0095664