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G1 Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells (2023)
Journal Article
Vassey, M., Firdaus, R., Aslam, A., Wheldon, L. M., Oldfield, N. J., Ala’Aldeen, D. A., & Wooldridge, K. G. (2023). G1 Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells. Cellular Microbiology, 2023, 1-15. https://doi.org/10.1155/2023/7480033

Neisseria meningitidis is the most frequent cause of bacterial meningitis and is one of the few bacterial pathogens that can breach the blood-brain barrier (BBB). The 37/67 kDa laminin receptor (LamR) was previously identified as a receptor mediating... Read More about G1 Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells.

Ferric Quinate (QPLEX) inhibits the interaction of Major Outer Membrane Protein (MOMP) with the Lewis b (Leb) antigen and Limits Campylobacter colonisation in broilers (2023)
Journal Article
Okoye, J. C., Holland, A., Pitoulias, M., Paschalis, V., Piddubnyi, A., Dufailu, O. A., …Soultanos, P. (2023). Ferric Quinate (QPLEX) inhibits the interaction of Major Outer Membrane Protein (MOMP) with the Lewis b (Leb) antigen and Limits Campylobacter colonisation in broilers. Frontiers in Microbiology, 14, Article 1146418. https://doi.org/10.3389/fmicb.2023.1146418

Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. jejuni. Here, we show that the essential major... Read More about Ferric Quinate (QPLEX) inhibits the interaction of Major Outer Membrane Protein (MOMP) with the Lewis b (Leb) antigen and Limits Campylobacter colonisation in broilers.

Ferric quinate (QPLEX) inhibits the interaction of major outer membrane protein (MOMP) with the Lewis b (Leb) antigen and limits Campylobacter colonization in broilers (2023)
Journal Article
Okoye, J. C., Holland, A., Pitoulias, M., Paschalis, V., Piddubnyi, A., Dufailu, O. A., …Soultanas, P. (2023). Ferric quinate (QPLEX) inhibits the interaction of major outer membrane protein (MOMP) with the Lewis b (Leb) antigen and limits Campylobacter colonization in broilers. Frontiers in Microbiology, 14, Article 1146418. https://doi.org/10.3389/fmicb.2023.1146418

Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. jejuni. Here, we show that the essential major... Read More about Ferric quinate (QPLEX) inhibits the interaction of major outer membrane protein (MOMP) with the Lewis b (Leb) antigen and limits Campylobacter colonization in broilers.

Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages (2023)
Journal Article
Dave, N., Albiheyri, R. S., Wanford, J. J., Green, L. R., Oldfield, N. J., Turner, D. P., …Bayliss, C. D. (2023). Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages. Microbiology, 169(2), https://doi.org/10.1099/mic.0.001305

Colonization of mucosal tissues by Neisseria meningitidis requires adhesion mediated by the type IV pilus and multiple outer-membrane proteins. Penetration of the mucosa and invasion of epithelial cells are thought to contribute to host persistence a... Read More about Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages.

Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space (2022)
Journal Article
Okoye, J. C., Bellamy-Carter, J., Oldham, N. J., Oldfield, N. J., Mahdavi, J., & Soultanas, P. (2022). Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space. Computational and Structural Biotechnology Journal, 20, 5355-5363. https://doi.org/10.1016/j.csbj.2022.09.032

Ferric chelates like ferric tyrosinate (TYPLEX) and the closely related ferric quinate (QPLEX) are structural mimics of bacterial siderophores. TYPLEX has been trialled as a feed additive in farming of commercial broilers, reducing Campylobacter load... Read More about Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space.

Impact of meningococcal ACWY conjugate vaccines on pharyngeal carriage in adolescents: evidence for herd protection from the UK MenACWY programme (2022)
Journal Article
Carr, J. P., MacLennan, J. M., Plested, E., Bratcher, H. B., Harrison, O. B., Aley, P. K., …Duale, K. (2022). Impact of meningococcal ACWY conjugate vaccines on pharyngeal carriage in adolescents: evidence for herd protection from the UK MenACWY programme. Clinical Microbiology and Infection, 28(12), 1649.e1-1649.e8. https://doi.org/10.1016/j.cmi.2022.07.004

Objective: Serogroup W and Y invasive meningococcal disease increased globally from 2000 onwards. Responding to a rapid increase in serogroup W clonal complex 11 (W:cc11) invasive meningococcal disease, the UK replaced an adolescent booster dose of m... Read More about Impact of meningococcal ACWY conjugate vaccines on pharyngeal carriage in adolescents: evidence for herd protection from the UK MenACWY programme.

Effect of priming interval on reactogenicity, peak immunological response, and waning after homologous and heterologous COVID-19 vaccine schedules: exploratory analyses of Com-COV, a randomised control trial (2022)
Journal Article
Shaw, R. H., Liu, X., Stuart, A. S. V., Greenland, M., Aley, P. K., Andrews, N. J., …Okenyi, E. (2022). Effect of priming interval on reactogenicity, peak immunological response, and waning after homologous and heterologous COVID-19 vaccine schedules: exploratory analyses of Com-COV, a randomised control trial. Lancet Respiratory Medicine, 10(11), 1049-1060. https://doi.org/10.1016/S2213-2600%2822%2900163-1

Background: Priming COVID-19 vaccine schedules have been deployed at variable intervals globally, which might influence immune persistence and the relative importance of third-dose booster programmes. Here, we report exploratory analyses from the Com... Read More about Effect of priming interval on reactogenicity, peak immunological response, and waning after homologous and heterologous COVID-19 vaccine schedules: exploratory analyses of Com-COV, a randomised control trial.

Safety and immunogenicity of heterologous versus homologous prime-boost schedules with an adenoviral vectored and mRNA COVID-19 vaccine (Com-COV): a single-blind, randomised, non-inferiority trial (2021)
Journal Article
Liu, X., Shaw, R. H., Stuart, A. S. V., Greenland, M., Aley, P. K., Andrews, N. J., …Allen, L. (2021). Safety and immunogenicity of heterologous versus homologous prime-boost schedules with an adenoviral vectored and mRNA COVID-19 vaccine (Com-COV): a single-blind, randomised, non-inferiority trial. Lancet, 398(10303), 856-869. https://doi.org/10.1016/S0140-6736%2821%2901694-9

Background: Use of heterologous prime-boost COVID-19 vaccine schedules could facilitate mass COVID-19 immunisation. However, we have previously reported that heterologous schedules incorporating an adenoviral vectored vaccine (ChAdOx1 nCoV-19, AstraZ... Read More about Safety and immunogenicity of heterologous versus homologous prime-boost schedules with an adenoviral vectored and mRNA COVID-19 vaccine (Com-COV): a single-blind, randomised, non-inferiority trial.

Rapid Transmission of a Hyper-Virulent Meningococcal Clone Due to High Effective Contact Numbers and Super Spreaders (2020)
Journal Article
Holmes, J. C., Green, L. R., Oldfield, N. J., Turner, D. P., & Bayliss, C. D. (2020). Rapid Transmission of a Hyper-Virulent Meningococcal Clone Due to High Effective Contact Numbers and Super Spreaders. Frontiers in Genetics, 11, https://doi.org/10.3389/fgene.2020.579411

© Copyright © 2020 Holmes, Green, Oldfield, Turner and Bayliss. Rapid transmission, a critical contributory factor in outbreaks of invasive meningococcal disease, requires naïve populations of sufficient size and intermingling. We examined genomic va... Read More about Rapid Transmission of a Hyper-Virulent Meningococcal Clone Due to High Effective Contact Numbers and Super Spreaders.

A role for fibroblast growth factor receptor 1 in the pathogenesis of Neisseria meningitidis (2020)
Journal Article
Azimi, S., Wheldon, L. M., Oldfield, N. J., Ala'Aldeen, D. A., & Wooldridge, K. G. (2020). A role for fibroblast growth factor receptor 1 in the pathogenesis of Neisseria meningitidis. Microbial Pathogenesis, 149, Article 104534. https://doi.org/10.1016/j.micpath.2020.104534

Neisseria meningitidis (the meningococcus) remains an important cause of human disease, including meningitis and sepsis. Adaptation to the host environment includes many interactions with specific cell surface receptors, resulting in intracellular si... Read More about A role for fibroblast growth factor receptor 1 in the pathogenesis of Neisseria meningitidis.

Localized Hypermutation is the Major Driver of Meningococcal Genetic Variability during Persistent Asymptomatic Carriage (2020)
Journal Article
Bayliss, C. D., Green, L. R., Al-Rubaiawi, A. A., Oldfield, N. J., Turner, D. P. J., Al-Maeni, M. A. R. M., …Maiden, M. C. J. (2020). Localized Hypermutation is the Major Driver of Meningococcal Genetic Variability during Persistent Asymptomatic Carriage. mBio, 11(2), Article e03068-19. https://doi.org/10.1128/mBio.03068-19

Host persistence of bacteria is facilitated by mutational and recombina-torial processes that counteract loss of genetic variation during transmission and selection from evolving host responses. Genetic variation was investigated during persistent as... Read More about Localized Hypermutation is the Major Driver of Meningococcal Genetic Variability during Persistent Asymptomatic Carriage.

Localized hypermutation is the major driver of meningococcal genetic variability during persistent asymptomatic carriage (2020)
Journal Article
Green, L. R., Al-Rubaiawi, A. A., Al-Maeni, M. A., Harrison, O. B., Blades, M., Oldfield, N. J., …Bayliss, C. D. (2020). Localized hypermutation is the major driver of meningococcal genetic variability during persistent asymptomatic carriage. mBio, 11(2), Article e03068-19. https://doi.org/10.1128/mBio.03068-19

© 2020 Green et al. Host persistence of bacteria is facilitated by mutational and recombina-torial processes that counteract loss of genetic variation during transmission and selection from evolving host responses. Genetic variation was investigated... Read More about Localized hypermutation is the major driver of meningococcal genetic variability during persistent asymptomatic carriage.

Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates (2019)
Journal Article
da Silva, R. A., Karlyshev, A. V., Oldfield, N. J., Wooldridge, K. G., Bayliss, C. D., Ryan, A., & Griffin, R. (2019). Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates. Frontiers in Microbiology, 10, https://doi.org/10.3389/fmicb.2019.02847

© Copyright © 2019 da Silva, Karlyshev, Oldfield, Wooldridge, Bayliss, Ryan and Griffin. Meningococcal lipoprotein, Factor H binding protein (FHbp), is the sole antigen of the Trumenba vaccine (Pfizer) and one of four antigens of the Bexsero vaccine... Read More about Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates.

The moonlighting peroxiredoxin-glutaredoxin in Neisseria meningitidis binds plasminogen via a C-terminal lysine residue and contributes to survival in a whole blood model (2019)
Journal Article
Aljannat, M. A., Oldfield, N. J., Albasri, H. M., Dorrington, L. K., Ohri, R. L., Wooldridge, K. G., & Turner, D. P. (2020). The moonlighting peroxiredoxin-glutaredoxin in Neisseria meningitidis binds plasminogen via a C-terminal lysine residue and contributes to survival in a whole blood model. Microbial Pathogenesis, 139, Article 103890. https://doi.org/10.1016/j.micpath.2019.103890

© 2019 Elsevier Ltd Neisseria meningitidis is a human-restricted bacterium that can invade the bloodstream and cross the blood-brain barrier resulting in life-threatening sepsis and meningitis. Meningococci express a cytoplasmic peroxiredoxin-glutare... Read More about The moonlighting peroxiredoxin-glutaredoxin in Neisseria meningitidis binds plasminogen via a C-terminal lysine residue and contributes to survival in a whole blood model.

Potentiation of phase variation in multiple outer membrane proteins during spread of the hyperinvasive Neisseria meningitidis serogroup W ST-11 lineage (2019)
Journal Article
Green, L. R., Dave, N., Adewoye, A. B., Lucidarme, J., Clark, S. A., Oldfield, N. J., …Bayliss, C. D. (2019). Potentiation of phase variation in multiple outer membrane proteins during spread of the hyperinvasive Neisseria meningitidis serogroup W ST-11 lineage. Journal of Infectious Diseases, 220(7), 1109–1117. https://doi.org/10.1093/infdis/jiz275

BACKGROUND: Since 2009, increases in invasive meningococcal disease have occurred in the United Kingdom due to a sub-lineage of the Neisseria meningitidis serogroup W ST-11 clonal complex (the 'original-UK' strain). In 2013, a descendent sub-strain... Read More about Potentiation of phase variation in multiple outer membrane proteins during spread of the hyperinvasive Neisseria meningitidis serogroup W ST-11 lineage.

Serogroup-specific meningococcal carriage by age group: a systematic review and meta-analysis (2019)
Journal Article
Peterson, M. E., Li, Y., Shanks, H., Mile, R., Nair, H., Kyaw, M. H., & Meningococcal Carriage Group. (2019). Serogroup-specific meningococcal carriage by age group: a systematic review and meta-analysis. BMJ Open, 9(4), Article e024343. https://doi.org/10.1136/bmjopen-2018-024343

Objective Neisseria meningitidis carriage prevalence has known variation across the lifespan, but it is unclear whether carriage varies among meningococcal capsular groups. Therefore, we aimed to characterise group-specific meningococcal carriage by... Read More about Serogroup-specific meningococcal carriage by age group: a systematic review and meta-analysis.

Limited impact of adolescent meningococcal ACWY vaccination on Neisseria meningitides serogroup W carriage in university students (2017)
Journal Article
Oldfield, N. J., Green, L. R., Parkhill, J., Bayliss, C. D., & Turner, D. P. (2018). Limited impact of adolescent meningococcal ACWY vaccination on Neisseria meningitides serogroup W carriage in university students. Journal of Infectious Diseases, 217(4), 608-616. https://doi.org/10.1093/infdis/jix596

Background In the UK rising disease levels due to Neisseria meningitidis serogroup W clonal complex ST-11 (MenW:cc11) strains led to introduction of conjugate MenACWY vaccination for teenagers. We investigated the impact of immunization on carriag... Read More about Limited impact of adolescent meningococcal ACWY vaccination on Neisseria meningitides serogroup W carriage in university students.

Rise in Group W Meningococcal Carriage in University Students, United Kingdom (2017)
Journal Article
Oldfield, N. J., Cayrou, C., AlJannat, M. A., Al-Rubaiawi, A. A., Green, L. R., Dada, S., …Turner, D. P. (2017). Rise in Group W Meningococcal Carriage in University Students, United Kingdom. Emerging Infectious Disease, 23(6), 1009-1011. https://doi.org/10.3201/eid2306.161768

MenACWY conjugate vaccination was recently introduced in the UK for adolescents and 24 young adults to reduce disease due to Neisseria meningitidis group W (MenW). We 25 conducted a cross-sectional carriage study in first year university students. De... Read More about Rise in Group W Meningococcal Carriage in University Students, United Kingdom.

Moonlighting Functions of Bacterial Fructose 1,6-Bisphosphate Aldolases (2017)
Book Chapter
Oldfield, N. J., Shams, F., Wooldridge, K. G., & Turner, D. P. (2017). Moonlighting Functions of Bacterial Fructose 1,6-Bisphosphate Aldolases. In B. Henderson (Ed.), Moonlighting Proteins: Novel Virulence Factors in Bacterial Infections (321-331). Wiley

© 2017 John Wiley & Sons, Inc. Moonlighting proteins are multifunctional proteins which perform two or more biochemical functions using a single polypeptide chain. Moonlighting proteins have been documented in diverse organisms, including archaea,... Read More about Moonlighting Functions of Bacterial Fructose 1,6-Bisphosphate Aldolases.

Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue (2016)
Journal Article
Shams, F., Oldfield, N. J., Lai, S. K., Tunio, S. A., Wooldridge, K. G., & Turner, D. P. (2016). Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue. Microbiology Open, 5(2), https://doi.org/10.1002/mbo3.331

Neisseria meningitidis is a leading cause of fatal sepsis and meningitis worldwide. As for commensal species of human neisseriae, N. meningitidis inhabits the human nasopharynx and asymptomatic colonization is ubiquitous. Only rarely does the organis... Read More about Fructose-1,6-bisphosphate aldolase of Neisseria meningitidis binds human plasminogen via its C-terminal lysine residue.

Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms (2016)
Journal Article
Oldfield, N. J., Harrison, O. B., Bayliss, C. D., Maiden, M. C., Ala'Aldeen, D. A., & Turner, D. P. (2016). Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms. Journal of Infectious Diseases, 213(11), 1777-1785. https://doi.org/10.1093/infdis/jiw008

Background. Neisseria meningitidis is a frequent colonizer of the human nasopharynx with asymptomatic carriage providing the reservoir for invasive, disease-causing strains. Serogroup Y (MenY) strains are a major cause of meningococcal disease. High... Read More about Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms.

Optimization of molecular approaches to genogroup Neisseria meningitidis carriage isolates and implications for monitoring the impact of new serogroup B vaccines (2015)
Journal Article
Rojas, E., Hoyos, J., Oldfield, N., Lee, P., Flint, M., Jones, C. H., …Anderson, A. S. (2015). Optimization of molecular approaches to genogroup Neisseria meningitidis carriage isolates and implications for monitoring the impact of new serogroup B vaccines. PLoS ONE, 10, Article e0132140. https://doi.org/10.1371/journal.pone.0132140

Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters (2015)
Journal Article
Khairalla, A. S., Omer, S. A., Mahdavi, J., Aslam, A., Dufailu, O. A., Self, T., …Ala'Aldeen, D. A. (2015). Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters. Cellular Microbiology, 17(7), 1008-1020. https://doi.org/10.1111/cmi.12417

Neisseria meningitidis, a major cause of bacterial meningitis and septicaemia, secretes multiple virulence factors, including the adhesion and penetration protein (App) and meningococcal serine protease A (MspA). Both are conserved, immunogenic, type... Read More about Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters.

Fructose-1,6-bisphosphate aldolase (FBA)-A conserved glycolytic enzyme with virulence functions in bacteria: 'Ill met by moonlight' (2014)
Journal Article
Shams, F., Oldfield, N. J., Wooldridge, K. G., & Turner, D. P. J. (2014). Fructose-1,6-bisphosphate aldolase (FBA)-A conserved glycolytic enzyme with virulence functions in bacteria: 'Ill met by moonlight'. Biochemical Society Transactions, 42(6), 1792-1795. https://doi.org/10.1042/BST20140203

© 2014 Biochemical Society. Moonlighting proteins constitute an intriguing class of multifunctional proteins. Metabolic enzymes and chaperones, which are often highly conserved proteins in bacteria, archaea and eukaryotic organisms, are among the mos... Read More about Fructose-1,6-bisphosphate aldolase (FBA)-A conserved glycolytic enzyme with virulence functions in bacteria: 'Ill met by moonlight'.

Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis (2014)
Journal Article
Alqahtani, F. Y. S., Mahdavi, J., Wheldon, L. M., Vassey, M., Pirinccioglu, N., Royer, P., …Ala'Aldeen, D. A. (2014). Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis. Open Biology, 4(10), Article 140053. https://doi.org/10.1098/rsob.140053

The non-integrin laminin receptor (LAMR1/RPSA) and galectin-3 (Gal-3) are multi-functional host molecules with roles in diverse pathological processes, particularly of infectious or oncogenic origins. Using bimolecular fluorescence complementation an... Read More about Deciphering the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis.

A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization (2014)
Journal Article
Mahdavi, J., Pirinccioglu, N., Oldfield, N. J., Carlsohn, E., Stoof, J., Aslam, A., …Ala'Aldeen, D. A. (2014). A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization. Open Biology, 4(1), Article 130202. https://doi.org/10.1098/rsob.130202

Campylobacter jejuni is an important cause of human foodborne gastroenteritis; strategies to prevent infection are hampered by a poor understanding of the complex interactions between host and pathogen. Previous work showed that C. jejuni could bind... Read More about A novel O-linked glycan modulates Campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization.

Prevalence and phase variable expression status of two autotransporters, NalP and MspA, in carriage and disease isolates of Neisseria meningitidis (2013)
Journal Article
Oldfield, N. J., Matar, S., Bidmos, F. A., Alamro, M., Neal, K. R., Turner, D. P., …Ala'Aldeen, D. A. (2013). Prevalence and phase variable expression status of two autotransporters, NalP and MspA, in carriage and disease isolates of Neisseria meningitidis. PLoS ONE, 8(7), Article e69746. https://doi.org/10.1371/journal.pone.0069746

Neisseria meningitidis is a human nasopharyngeal commensal capable of causing life-threatening septicemia and meningitis. Many meningococcal surface structures, including the autotransporter proteins NalP and MspA, are subject to phase variation (PV)... Read More about Prevalence and phase variable expression status of two autotransporters, NalP and MspA, in carriage and disease isolates of Neisseria meningitidis.

Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2 (2012)
Journal Article
Abouseada, N. M., Assafi, M. S. A., Mahdavi, J., Oldfield, N. J., Wheldon, L. M., Wooldridge, K. G., & Ala'Aldeen, D. A. (2012). Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2. PLoS ONE, 7(9), Article 10. https://doi.org/10.1371/journal.pone.0046233

Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae are major bacterial agents of meningitis. They each bind the 37/67-kDa laminin receptor (LamR) via the surface protein adhesins: meningococcal PilQ and PorA, H. influenzae Om... Read More about Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2.

Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni (2012)
Journal Article
Javed, M. A., Cawthraw, S. A., Baig, A., Li, J., McNally, A., Oldfield, N. J., …Manning, G. (2012). Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni. Infection and Immunity, 80(7), 2361-2370. https://doi.org/10.1128/IAI.00151-12

Campylobacter jejuni is a major cause of bacterial food-borne enteritis worldwide, and invasion into intestinal epithelial cells is an important virulence mechanism. Recently we reported the identification of hyperinvasive C. jejuni strains and creat... Read More about Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni.

Persistence, replacement, and rapid clonal expansion of meningococcal carriage isolates in a 2008 university student cohort (2011)
Journal Article
Bidmos, F. A., Neal, K. R., Oldfield, N. J., Turner, D. P., Ala'Aldeen, D. A., & Bayliss, C. D. (2011). Persistence, replacement, and rapid clonal expansion of meningococcal carriage isolates in a 2008 university student cohort. Journal of Clinical Microbiology, 49(2), 506-512. https://doi.org/10.1128/JCM.01322-10

A study of meningococcal carriage dynamics was performed with a cohort of 190 first-year students recruited from six residential halls at Nottingham University, United Kingdom. Pharyngeal swabs were obtained on four occasions between November 2008 an... Read More about Persistence, replacement, and rapid clonal expansion of meningococcal carriage isolates in a 2008 university student cohort.

The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells (2010)
Journal Article
Tunio, S. A., Oldfield, N. J., Ala'Aldeen, D. A. A., Wooldridge, K. G., & Turner, D. P. J. (2010). The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells. BMC Microbiology, 10, Article 280. https://doi.org/10.1186/1471-2180-10-280

Background Glyceraldehyde 3-phosphate dehydrogenases (GAPDHs) are cytoplasmic glycolytic enzymes, which although lacking identifiable secretion signals, have also been found localized to the surface of several bacteria (and some eukaryotic organisms... Read More about The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells.

Human antibody responses to the meningococcal factor H binding protein (LP2086) during invasive disease, colonization and carriage (2010)
Journal Article
Ala'Aldeen, D. A., Flint, M., Oldfield, N. J., Omer, S. A., McNeil, L. K., Jiang, Q., …Anderson, A. S. (2010). Human antibody responses to the meningococcal factor H binding protein (LP2086) during invasive disease, colonization and carriage. Vaccine, 28(48), 7667-7675. https://doi.org/10.1016/j.vaccine.2010.09.038

Recombinant forms of Neisseria meningitidis factor H binding protein (fHBP) are undergoing clinical trials in candidate vaccines against serogroup B meningococcal disease. Little is known, however, about the host response to fHBP during natural carri... Read More about Human antibody responses to the meningococcal factor H binding protein (LP2086) during invasive disease, colonization and carriage.

The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: Surface localization and role in host cell adhesion (2010)
Journal Article
Tunio, S. A., Oldfield, N. J., Berry, A., Ala'Aldeen, D. A. A., Wooldridge, K. G., & Turner, D. P. J. (2010). The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: Surface localization and role in host cell adhesion. Molecular Microbiology, 76(3), 605-615. https://doi.org/10.1111/j.1365-2958.2010.07098.x

Fructose-1, 6-bisphosphate aldolases (FBA) are cytoplasmic glycolytic enzymes, which despite lacking identifiable secretion signals, have also been found localized to the surface of several bacteria where they bind host molecules and exhibit non-glyc... Read More about The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: Surface localization and role in host cell adhesion.

AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge (2009)
Journal Article
Oldfield, N. J., Worrall, K. E., Rycroft, A. N., Ali, T., Wooldridge, K. G., & Ala'Aldeen, D. A. (2009). AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge. Vaccine, 27(38), 5278-5283. https://doi.org/10.1016/j.vaccine.2009.06.047

Actinobacillus pleuropneumoniae is a major respiratory pathogen of pigs; current vaccines provide only limited protection. AasP, a subtilisin-like serine protease, is a conserved outer membrane-localised autotransporter protein. We hypothesized that... Read More about AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge.

Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models (2009)
Journal Article
Orihuela, C. J., Mahdavi, J., Thornton, J., Mann, B., Wooldridge, K. G., Abouseada, N., …Tuomanen, E. I. (2009). Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation, 119(6), 1638-1646. https://doi.org/10.1172/JCI36759

A diverse array of infectious agents, including prions and certain neurotropic viruses, bind to the laminin receptor (LR), and this determines tropism to the CNS. Bacterial meningitis in childhood is almost exclusively caused by the respiratory tract... Read More about Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models.

Functional Characterization of AasP, a Maturation Protease Autotransporter Protein of Actinobacillus pleuropneumoniae (2008)
Journal Article
Ali, T., Oldfield, N. J., Wooldridge, K. G., Turner, D. P., & Ala'Aldeen, D. A. A. (2008). Functional Characterization of AasP, a Maturation Protease Autotransporter Protein of Actinobacillus pleuropneumoniae. Infection and Immunity, 76(12), 5608-5614. https://doi.org/10.1128/IAI.00085-08

Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a highly contagious respiratory infection in pigs. AasP, a putative subtilisin-like serine protease autotransporter, has recently been identified in A. pleuropneumon... Read More about Functional Characterization of AasP, a Maturation Protease Autotransporter Protein of Actinobacillus pleuropneumoniae.

Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae (2008)
Journal Article
Oldfield, N. J., Donovan, E. A., Worrall, K. E., Wooldridge, K. G., Langford, P. R., Rycroft, A. N., & Ala'Aldeen, D. A. (2008). Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae. Vaccine, 26(16), 1942-1954. https://doi.org/10.1016/j.vaccine.2008.02.022

Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine com... Read More about Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae.

CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut (2007)
Journal Article
Ashgar, S. S., Oldfield, N. J., Wooldridge, K. G., Jones, M. A., Irving, G. J., Turner, D. P., & Ala'Aldeen, D. A. (2007). CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut. Journal of Bacteriology, 189(5), 1856-1865. https://doi.org/10.1128/JB.01427-06

Two putative autotransporter proteins, CapA and CapB, were identified in silico from the genome sequence of Campylobacter jejuni NCTC11168. The genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slip... Read More about CapA, an Autotransporter Protein of Campylobacter jejuni, Mediates Association with Human Epithelial Cells and Colonization of the Chicken Gut.

T-cell stimulating protein A (TspA) of Neisseria meningitidis is required for optimal adhesion to human cells (2006)
Journal Article
Oldfield, N. J., Bland, S. J., Taraktsoglou, M., Dos Ramos, F. J., Robinson, K., Wooldridge, K. G., & Ala'Aldeen, D. A. (2007). T-cell stimulating protein A (TspA) of Neisseria meningitidis is required for optimal adhesion to human cells. Cellular Microbiology, 9(2), 463-478. https://doi.org/10.1111/j.1462-5822.2006.00803.x

T-cell stimulating protein A (TspA) is an immunogenic, T-cell and B-cell stimulating protein of Neisseria meningitidis. Sequence similarity between TspA and FimV, a Pseudomonas aeruginosa protein involved in twitching motility, suggested a link betwe... Read More about T-cell stimulating protein A (TspA) of Neisseria meningitidis is required for optimal adhesion to human cells.

Characterization of the Campylobacter jejuni Heptosyltransferase II Gene, waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent (2002)
Journal Article
Oldfield, N. J., Moran, A. P., Millar, L. A., Prendergast, M. M., & Ketley, J. M. (2002). Characterization of the Campylobacter jejuni Heptosyltransferase II Gene, waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent. Journal of Bacteriology, 184(8), 2100-2107. https://doi.org/10.1128/JB.184.8.2100-2107.2002

Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C jejuni-mediated enteric disease is unclear; however, epitopes... Read More about Characterization of the Campylobacter jejuni Heptosyltransferase II Gene, waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent.

Cloning, mutation and distribution of a putative lipopolysaccharide biosynthesis locus in Campylobacter jejuni (1999)
Journal Article
Wood, A. C., Oldfield, N. J., O'Dwyer, C. A., & Ketley, J. M. (1999). Cloning, mutation and distribution of a putative lipopolysaccharide biosynthesis locus in Campylobacter jejuni. Microbiology, 145(2), 379-388. https://doi.org/10.1099/13500872-145-2-379

A region encoding ORFs with homology to known lipopolysaccharide (LPS) biosynthesis genes was isolated from two strains of Campylobacter jejuni. One of the strains produces LPS, but the second strain is reported to produce only lipooligosaccharide (L... Read More about Cloning, mutation and distribution of a putative lipopolysaccharide biosynthesis locus in Campylobacter jejuni.