Jennifer C. Okoye
Ferric Quinate (QPLEX) inhibits the interaction of Major Outer Membrane Protein (MOMP) with the Lewis b (Leb) antigen and Limits Campylobacter colonisation in broilers
Okoye, Jennifer C.; Holland, Alexandria; Pitoulias, Matthaios; Paschalis, Vasileios; Piddubnyi, Artem; Dufailu, Osman A.; Boren, Thomas; Oldfield, Neil J.; Mahdari, Jafar; Soultanos, Panos
Authors
Alexandria Holland
Matthaios Pitoulias
Vasileios Paschalis
Artem Piddubnyi
Osman A. Dufailu
Thomas Boren
Dr NEIL OLDFIELD NEIL.OLDFIELD@NOTTINGHAM.AC.UK
ASSISTANT PROFESSOR
Jafar Mahdari
Panos Soultanos
Abstract
Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. jejuni. Here, we show that the essential major outer membrane protein (MOMP) of C. jejuni NCTC11168 binds to the Lewis b (Leb) antigen on the gastrointestinal epithelia of host tissues and this interaction can be competitively inhibited by ferric quinate (QPLEX), a ferric chelate structurally similar to bacterial siderophores. We provide evidence that QPLEX competitively inhibits the MOMP-Leb interaction. Furthermore, we demonstrate that QPLEX can be used as a feed additive in broiler farming to significantly reduce C. jejuni colonization. Our results indicate that QPLEX can be a viable alternative to the preventative use of antibiotics in broiler farming to combat C. jejuni infections.
Citation
Okoye, J. C., Holland, A., Pitoulias, M., Paschalis, V., Piddubnyi, A., Dufailu, O. A., Boren, T., Oldfield, N. J., Mahdari, J., & Soultanos, P. (2023). Ferric Quinate (QPLEX) inhibits the interaction of Major Outer Membrane Protein (MOMP) with the Lewis b (Leb) antigen and Limits Campylobacter colonisation in broilers. Frontiers in Microbiology, 14, Article 1146418. https://doi.org/10.3389/fmicb.2023.1146418
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 27, 2023 |
Online Publication Date | Mar 10, 2023 |
Publication Date | 2023 |
Deposit Date | Mar 9, 2023 |
Publicly Available Date | Mar 10, 2023 |
Journal | Frontiers in Microbiology |
Electronic ISSN | 1664-302X |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Article Number | 1146418 |
DOI | https://doi.org/10.3389/fmicb.2023.1146418 |
Public URL | https://nottingham-repository.worktribe.com/output/18232673 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1146418/full |
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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