All Outputs (2255)

Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou‐Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D. O., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well‐characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase.

Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou-Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well-characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase.

Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids (2023)
Journal Article
Kalli, S., Vallieres, C., Violet, J., Sanders, J., Chapman, J., Vincken, J., …Araya-Cloutier, C. (2023). Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids. Microbiology Spectrum, 11(4), Article e0132723. https://doi.org/10.1128/spectrum.01327-23

Prenylated isoflavonoids are phytochemicals with promising antifungal properties. Recently, it was shown that glabridin and wighteone disrupted the plasma membrane (PM) of the food spoilage yeast Zygosaccharomyces parabailii in distinct ways, which l... Read More about Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids.

A new species of the genus Anamylopsora (Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan (2023)
Journal Article
Usman, M., Firdous, Q., Dyer, P. S., & Khalid, A. N. (2023). A new species of the genus Anamylopsora (Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan. Lichenologist, https://doi.org/10.1017/s002428292300018x

A novel lichen species occurring on rocks was collected from three different localities within Deosai National Park, Gilgit-Baltistan, Pakistan. Phylogenetic analyses of the nrDNA ITS and nuLSU regions revealed that it clustered within the genus Anam... Read More about A new species of the genus Anamylopsora (Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan.

Associations of obesity phenotypes with weight change, cardiometabolic benefits, and type 2 diabetes incidence during a lifestyle intervention: results from the PREVIEW study (2023)
Journal Article
Zhu, R., Huttunen-Lenz, M., Stratton, G., Handjieva-Darlenska, T., Handjiev, S., Sundvall, J., …Raben, A. (2023). Associations of obesity phenotypes with weight change, cardiometabolic benefits, and type 2 diabetes incidence during a lifestyle intervention: results from the PREVIEW study. International Journal of Obesity, 47(9), 833–840. https://doi.org/10.1038/s41366-023-01328-y

Background/Objectives Some individuals with overweight/obesity may be relatively metabolically healthy (MHO) and have a lower risk of cardiovascular disease than those with metabolically unhealthy overweight/obesity (MUO). We aimed to compare change... Read More about Associations of obesity phenotypes with weight change, cardiometabolic benefits, and type 2 diabetes incidence during a lifestyle intervention: results from the PREVIEW study.

Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article
Lever, R., Simmons, E., Gamble-Milner, R., Buckley, R., Harrison, C., Parkes, A., …Allers, T. (2023). Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing. Nucleic Acids Research, https://doi.org/10.1093/nar/gkad572

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET (2023)
Journal Article
Van Daele, M., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2023). Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET. Biochemical Pharmacology, 214, Article 115672. https://doi.org/10.1016/j.bcp.2023.115672

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis, proliferation and migration of vascular endothelial cells. It is well known that cardiovascular safety liability for a wide range of small molecule tyrosine kinase in... Read More about Characterisation of tyrosine kinase inhibitor-receptor interactions at VEGFR2 using sunitinib-red and nanoBRET.

Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells (2023)
Journal Article
Ogrodzinski, L., Platt, S., Goulding, J., Alexander, C., Farr, T. D., Woolard, J., …Kilpatrick, L. E. (2023). Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells. iScience, 26(7), Article 107232. https://doi.org/10.1016/j.isci.2023.107232

E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in living c... Read More about Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells.

The evolution and ecology of multiple antipredator defences (2023)
Journal Article
Kikuchi, D. W., Allen, W. L., Arbuckle, K., Aubier, T. G., Briolat, E. S., Burdfield-Steel, E. R., …Exnerová, A. (2023). The evolution and ecology of multiple antipredator defences. Journal of Evolutionary Biology, 36(7), 975-991. https://doi.org/10.1111/jeb.14192

Prey seldom rely on a single type of antipredator defence, often using multiple defences to avoid predation. In many cases, selection in different contexts may favour the evolution of multiple defences in a prey. However, a prey may use multiple defe... Read More about The evolution and ecology of multiple antipredator defences.

Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum (2023)
Journal Article
Poulalier-Delavelle, M., Baker, J. P., Millard, J., Winzer, K., & Minton, N. P. (2023). Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum. Frontiers in Bioengineering and Biotechnology, 11, Article 1213236. https://doi.org/10.3389/fbioe.2023.1213236

Acetogenic bacteria can play a major role in achieving Net Zero through their ability to convert CO2 into industrially relevant chemicals and fuels. Full exploitation of this potential will be reliant on effective metabolic engineering tools, such as... Read More about Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum.

Extracellular vesicle microRNA mediated transcriptional regulation may contribute to Dementia with Lewy Bodies molecular pathology (2023)
Journal Article
Işık, F. B., Knight, H. M., & Rajkumar, A. P. (2024). Extracellular vesicle microRNA mediated transcriptional regulation may contribute to Dementia with Lewy Bodies molecular pathology. Acta Neuropsychiatrica, 36(1), 29-38. https://doi.org/10.1017/neu.2023.27

Objective: Dementia with Lewy bodies (DLB) is the second most common dementia. Advancing our limited understanding of its molecular pathogenesis is essential for identifying novel biomarkers and therapeutic targets for DLB. DLB is an α-synucleinop... Read More about Extracellular vesicle microRNA mediated transcriptional regulation may contribute to Dementia with Lewy Bodies molecular pathology.

Reactogenicity, immunogenicity and breakthrough infections following heterologous or fractional second dose COVID-19 vaccination in adolescents (Com-COV3): A randomised controlled trial (2023)
Journal Article
Kelly, E., Greenland, M., de Whalley, P. C., Aley, P. K., Plested, E. L., Singh, N., …Com-COV3 Study Group, C. S. G. (2023). Reactogenicity, immunogenicity and breakthrough infections following heterologous or fractional second dose COVID-19 vaccination in adolescents (Com-COV3): A randomised controlled trial. Journal of Infection, 87(3), 230-241. https://doi.org/10.1016/j.jinf.2023.06.007

Background: This was the first study to investigate the reactogenicity and immunogenicity of heterologous or fractional second dose COVID-19 vaccine regimens in adolescents. Methods: A phase II, single-blind, multi-centre, randomised-controlled trial... Read More about Reactogenicity, immunogenicity and breakthrough infections following heterologous or fractional second dose COVID-19 vaccination in adolescents (Com-COV3): A randomised controlled trial.

Sarbecoviruses of British Horseshoe Bats; Sequence Variation and Epidemiology (2023)
Journal Article
Apaa, T., Withers, A. J., Staley, C. E., Blanchard, A. M., Bennett, M., Bremner-Harrison, S., …Tarlinton, R. E. (2023). Sarbecoviruses of British Horseshoe Bats; Sequence Variation and Epidemiology. Journal of General Virology, 104(6), Article 001859. https://doi.org/10.1101/2023.02.14.528476

Horseshoe bats are the natural hosts of the Sarbecovirus subgenus that includes SARS-CoV and SARS-CoV- 2. Despite the devastating impact of the COVID-19 pandemic, there is still little known about the underlying epidemiology and virology of sarbecovi... Read More about Sarbecoviruses of British Horseshoe Bats; Sequence Variation and Epidemiology.

NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl (2023)
Journal Article
Simpson, L. A., Crowley, D., Forey, T., Acosta, H., Ferjentsik, Z., Chatfield, J., …Johnson, A. D. (2023). NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl. PLoS Biology, 21(6), Article e3002121. https://doi.org/10.1371/journal.pbio.3002121

Pluripotency defines the unlimited potential of individual cells of vertebrate embryos, from which all adult somatic cells and germ cells are derived. Understanding how the programming of pluripotency evolved has been obscured in part by a lack of da... Read More about NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl.

Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation (2023)
Journal Article
Killelea, T., Dimude, J. U., He, L., Stewart, A. L., Kemm, F. E., Radovčí, M., …Bolt, E. L. (2023). Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation. Nucleic Acids Research, 51(13), 6914-6926. https://doi.org/10.1093/nar/gkad473

Prokaryotic Cas1-Cas2 protein complexes generate adaptive immunity to mobile genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De novo immunity relies on naive adaptation-Cas1-Cas2 targeting of MGE DNA without the aid... Read More about Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation.

Increasing SARS-CoV-2 seroprevalence among UK pediatric patients on dialysis and kidney transplantation between January 2020 and August 2021 (2023)
Journal Article
Bamber, H. N., Kim, J. J., Reynolds, B. C., Afzaal, J., Lunn, A. J., Tighe, P. J., …Tarr, A. W. (2023). Increasing SARS-CoV-2 seroprevalence among UK pediatric patients on dialysis and kidney transplantation between January 2020 and August 2021. Pediatric Nephrology, 38, 3745-3755. https://doi.org/10.1007/s00467-023-05983-1

Background Coronavirus disease 2019 (COVID-19) was officially declared a pandemic by the World Health Organisation (WHO) on 11 March 2020, as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread rapidly across the world. We investiga... Read More about Increasing SARS-CoV-2 seroprevalence among UK pediatric patients on dialysis and kidney transplantation between January 2020 and August 2021.

Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS (2023)
Journal Article
Khateb, H., Hook, A. L., Kern, S., Watts, J. A., Singh, S., Jackson, D., …Alexander, M. R. (2023). Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS. Biointerphases, 18(3), Article 031007. https://doi.org/10.1116/6.0002604

Secondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preser... Read More about Identification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMS.

The Lanthipeptide Synthetase-like Protein CA_C0082 Is an Effector of Agr Quorum Sensing in Clostridium acetobutylicum (2023)
Journal Article
Humphreys, J. R., Bean, Z., Twycross, J., & Winzer, K. (2023). The Lanthipeptide Synthetase-like Protein CA_C0082 Is an Effector of Agr Quorum Sensing in Clostridium acetobutylicum. Microorganisms, 11(6), Article 1460. https://doi.org/10.3390/microorganisms11061460

Lanthipeptide synthetases are present in all domains of life. They catalyze a crucial step during lanthipeptide biosynthesis by introducing thioether linkages during posttranslational peptide modification. Lanthipeptides have a wide range of function... Read More about The Lanthipeptide Synthetase-like Protein CA_C0082 Is an Effector of Agr Quorum Sensing in Clostridium acetobutylicum.

Modelling spatiotemporal dynamics of cerebral blood flow using multiple-timepoint arterial spin labelling MRI (2023)
Journal Article
Pinto, J., Blockley, N. P., Harkin, J. W., & Bulte, D. P. (2023). Modelling spatiotemporal dynamics of cerebral blood flow using multiple-timepoint arterial spin labelling MRI. Frontiers in Physiology, 14, Article 1142359. https://doi.org/10.3389/fphys.2023.1142359

Introduction: Cerebral blood flow (CBF) is an important physiological parameter that can be quantified non-invasively using arterial spin labelling (ASL) imaging. Although most ASL studies are based on single-timepoint strategies, multi-timepoint app... Read More about Modelling spatiotemporal dynamics of cerebral blood flow using multiple-timepoint arterial spin labelling MRI.

Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes (2023)
Journal Article
Lay, C. S., Isidro-Llobet, A., Kilpatrick, L. E., Craggs, P. D., & Hill, S. J. (2023). Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes. Nature Communications, 14, Article 2882. https://doi.org/10.1038/s41467-023-38541-2

Association of single nucleotide polymorphisms in the IL-23 receptor with several auto-inflammatory diseases, led to the heterodimeric receptor and its cytokine-ligand IL-23, becoming important drug targets. Successful antibody-based therapies direct... Read More about Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes.