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A spoonful of sugar: the application of glycopolymers in therapeutics (2011)
Journal Article
Spain, S. G., & Cameron, N. R. (2011). A spoonful of sugar: the application of glycopolymers in therapeutics. Polymer Chemistry, 2011(2), https://doi.org/10.1039/c0py00149j

Glycopolymers, synthetic polymers displaying carbohydrate moieties, have been linked to many potential applications at the biology–chemistry interface. One area that holds particular promise is the employment of glycopolymers as vehicles for therapeu... Read More about A spoonful of sugar: the application of glycopolymers in therapeutics.

Predicting beta-turns and their types using predicted backbone dihedral angles and secondary structures (2010)
Journal Article
Kountouris, P., & Hirst, J. D. (2010). Predicting beta-turns and their types using predicted backbone dihedral angles and secondary structures. BMC Bioinformatics, 11, Article 407. https://doi.org/10.1186/1471-2105-11-407

Background β-turns are secondary structure elements usually classified as coil. Their prediction is important, because of their role in protein folding and their frequent occurrence in protein chains. Results We have developed a novel method... Read More about Predicting beta-turns and their types using predicted backbone dihedral angles and secondary structures.

Chemical Tags Mediate the Orthogonal Self-Assembly of DNA Duplexes into Supramolecular Structures (2010)
Journal Article
Mitchell, N. J., Ebner, A., Hinterdorfer, P., Tampé, R., & Howorka, S. (2010). Chemical Tags Mediate the Orthogonal Self-Assembly of DNA Duplexes into Supramolecular Structures. Small, 6(16), 1732-1735. https://doi.org/10.1002/smll.201000790

A new chemical approach that achieves ordered self?assembly of biomolecules is reported. The chemical tags bis?nitrilotriacetic acid (bisNTA) and hexahistidine (His6) are attached to DNA duplexes that self?assemble into supramolecular structures whos... Read More about Chemical Tags Mediate the Orthogonal Self-Assembly of DNA Duplexes into Supramolecular Structures.

DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis (2010)
Journal Article
Grainger, W. H., Machón, C., Scott, D. J., & Soultanas, P. (2010). DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis. Nucleic Acids Research, 38(9), https://doi.org/10.1093/nar/gkp1236

Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD... Read More about DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis.

RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD (2010)
Journal Article
Mach?n, C., Thomson, N., Lynch, G., Scott, D. J., Thomas, C., & Soultanas, P. (2010). RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD. Nucleic Acids Research, 38(6), https://doi.org/10.1093/nar/gkp1153

Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its pro... Read More about RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD.

Predicting ?-turns and their types using predicted backbone dihedral angles and secondary structures (2010)
Journal Article
Kountouris, P., & Hirst, J. (2010). Predicting ?-turns and their types using predicted backbone dihedral angles and secondary structures. BMC Bioinformatics, 11,

Background: β-turns are secondary structure elements usually classified as coil. Their prediction is important, because of their role in protein folding and their frequent occurrence in protein chains. Results: We have developed a novel method tha... Read More about Predicting ?-turns and their types using predicted backbone dihedral angles and secondary structures.

Exploring protein structural dissimilarity to facilitate structure classification (2009)
Journal Article
Jain, P., & Hirst, J. (2009). Exploring protein structural dissimilarity to facilitate structure classification. BMC Structural Biology, 9, https://doi.org/10.1186/1472-6807-9-60

Background: Classification of newly resolved protein structures is important in understanding their architectural, evolutionary and functional relatedness to known protein structures. Among various efforts to improve the database of Structural Classi... Read More about Exploring protein structural dissimilarity to facilitate structure classification.

Chemically Labeled Nucleotides and Oligonucleotides Encode DNA for Sensing with Nanopores (2009)
Journal Article
Borsenberger, V., Mitchell, N. J., & Howorka, S. (2009). Chemically Labeled Nucleotides and Oligonucleotides Encode DNA for Sensing with Nanopores. Journal of the American Chemical Society, 131(22), 7530-7531. https://doi.org/10.1021/ja902004s

The labeling of nucleotides and oligonucleotides with reporter groups is an important tool in the sequence-specific sensing of DNA, as exemplified by fluorescence tags. Here we show that chemical tags can encode sequence information for electrical na... Read More about Chemically Labeled Nucleotides and Oligonucleotides Encode DNA for Sensing with Nanopores.

Bis-morpholine-substituted perylene bisimides: Impact of isomeric arrangement on electrochemical and spectroelectrochemical properties (2008)
Journal Article
Goretzki, G., Davies, E. S., Argent, S. P., Alsindi, W. Z., Blake, A. J., Warren, J. E., …Champness, N. R. (2008). Bis-morpholine-substituted perylene bisimides: Impact of isomeric arrangement on electrochemical and spectroelectrochemical properties. Journal of Organic Chemistry, 73(22), 8808-8814. https://doi.org/10.1021/jo801557e

(Graph Presented) The synthesis and separation of the 1,6- and 1,7- isomers of N,N′-bis(n-butyl)dimorpholino-3,4:9,10-perylenetetracarboxylic acid bisimide are reported. Investigations of the electrochemical and spectroscopic, in particular, spectroe... Read More about Bis-morpholine-substituted perylene bisimides: Impact of isomeric arrangement on electrochemical and spectroelectrochemical properties.

Chemical Tags Facilitate the Sensing of Individual DNA Strands with Nanopores (2008)
Journal Article
Mitchell, N. J., & Howorka, S. (2008). Chemical Tags Facilitate the Sensing of Individual DNA Strands with Nanopores. Angewandte Chemie International Edition, 47(30), 5565-5568. https://doi.org/10.1002/anie.200800183

The base resolution in the detection of single DNA strands with nanopores can be improved by attaching chemical tags to individual bases. Different chemical tags give rise to characteristic current signatures when a DNA molecule passes through a nano... Read More about Chemical Tags Facilitate the Sensing of Individual DNA Strands with Nanopores.

Structure of the N-terminal oligomerization domain of DnaD reveals a unique tetramerization motif and provides insights into scaffold formation (2008)
Journal Article
Schneider, S., Zhang, W., Soultanas, P., & Paoli, M. (2008). Structure of the N-terminal oligomerization domain of DnaD reveals a unique tetramerization motif and provides insights into scaffold formation. Journal of Molecular Biology, 376(5),

DnaD is a primosomal protein that remodels supercoiled plasmids. It binds to supercoiled forms and converts them to open forms without nicking. During this remodeling process, all the writhe is converted to twist and the plasmids are held around the... Read More about Structure of the N-terminal oligomerization domain of DnaD reveals a unique tetramerization motif and provides insights into scaffold formation.

Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus (2006)
Journal Article
Warfield, R., Bardelang, P., Saunders, H., Chan, W. C., Penfold, C., James, R., & Thomas, N. R. (2006). Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus. Organic and Biomolecular Chemistry, 4(19), 3626-3638. https://doi.org/10.1039/b607999g

Lysostaphin (EC. 3.4.24.75) is a protein secreted by Staphylococcus simulans biovar staphylolyticus and has been shown to be active against methicillin resistant S. aureus (MRSA). The design and synthesis of three internally quenched substrates for l... Read More about Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus.

The Bacillus subtilis primosomal protein DnaD untwists supercoiled DNA (2006)
Journal Article
Zhang, W., Allen, S., Roberts, C. J., & Soultanas, P. (2006). The Bacillus subtilis primosomal protein DnaD untwists supercoiled DNA. Journal of Bacteriology, 188(15), https://doi.org/10.1128/JB.00339-06

The essential Bacillus subtilis DnaD and DnaB proteins have been implicated in the initiation of DNA replication. Recently, DNA remodeling activities associated with both proteins were discovered that could provide a link between global or local nucl... Read More about The Bacillus subtilis primosomal protein DnaD untwists supercoiled DNA.

A Porous Framework Polymer Based on a Zinc(II) 4,4‘-Bipyridine-2,6,2‘,6‘-tetracarboxylate:  Synthesis, Structure, and “Zeolite-Like” Behaviors (2006)
Journal Article
Lin, X., Blake, A. J., Wilson, C., Sun, X. Z., Champness, N. R., George, M. W., …Schröder, M. (2006). A Porous Framework Polymer Based on a Zinc(II) 4,4‘-Bipyridine-2,6,2‘,6‘-tetracarboxylate:  Synthesis, Structure, and “Zeolite-Like” Behaviors. Journal of the American Chemical Society, 128(33), 10745-10753. https://doi.org/10.1021/ja060946u

The robust metal-organic framework compound {[Zn2(L)] ·4H2O}∞ I has been synthesized by hydrothermal reaction of ZnCl2 and 4,4′-bipyridine-2,6, 2′,6′-tetracarboxylic acid (H4L). Compound I crystallizes in a chiral space group, P42212, with the chiral... Read More about A Porous Framework Polymer Based on a Zinc(II) 4,4‘-Bipyridine-2,6,2‘,6‘-tetracarboxylate:  Synthesis, Structure, and “Zeolite-Like” Behaviors.

Asymmetric reduction of ?, ?-unsaturated ketone to (R) allylic alcohol by Candida chilensis (2006)
Journal Article
Pollard, D. J., Telari, K., Lane, J., Humphrey, G., McWilliams, C., Nidositko, S., …Moore, J. (2006). Asymmetric reduction of α, β-unsaturated ketone to (R) allylic alcohol by Candida chilensis. Biotechnology and Bioengineering, 93(4), 674-686. https://doi.org/10.1002/bit.20751

A pilot scale whole cell process was developed for the enantioselective 1,2‐reduction of prochiral α,β‐unsaturated ketone to (R) allylic alcohol using Candida chilensis. Initial development showed high enantiomeric excess (EE > 95%) but low product y... Read More about Asymmetric reduction of ?, ?-unsaturated ketone to (R) allylic alcohol by Candida chilensis.

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis (2006)
Journal Article
Ioannou, C., Schaeffer, P. M., Dixon, N. E., & Soultanas, P. (2006). Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis. Nucleic Acids Research, 34(18), https://doi.org/10.1093/nar/gkl690

The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-termina... Read More about Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis.

The bacterial helicase-primase interaction: a common structural/functional module (2005)
Journal Article
Soultanas, P. (2005). The bacterial helicase-primase interaction: a common structural/functional module. Structure, 13(6), https://doi.org/10.1016/j.str.2005.04.006

The lack of a high-resolution structure for the bacterial helicase-primase complex and the fragmented structural information for the individual proteins have been hindering our detailed understanding of this crucial binary protein interaction. Two ne... Read More about The bacterial helicase-primase interaction: a common structural/functional module.

Discovery of antagonist peptides against bacterial helicase-primase interaction in B. stearothermophilus by reverse yeast three-hybrid (2005)
Journal Article
Gardiner, L., Coyle, B. J., Chan, W. C., & Soultanas, P. (2005). Discovery of antagonist peptides against bacterial helicase-primase interaction in B. stearothermophilus by reverse yeast three-hybrid. Cell Chemistry Biology, 12(5), 595-604. https://doi.org/10.1016/j.chembiol.2005.04.007

Developing small-molecule antagonists against protein-protein interactions will provide powerful tools for mechanistic/functional studies and the discovery of new antibacterials. We have developed a reverse yeast three-hybrid approach that allows hig... Read More about Discovery of antagonist peptides against bacterial helicase-primase interaction in B. stearothermophilus by reverse yeast three-hybrid.