Chemically Labeled Nucleotides and Oligonucleotides Encode DNA for Sensing with Nanopores

Borsenberger, Vinciane; Mitchell, Nicholas J.; Howorka, Stefan

Abstract

The labeling of nucleotides and oligonucleotides with reporter groups is an important tool in the sequence-specific sensing of DNA, as exemplified by fluorescence tags. Here we show that chemical tags can encode sequence information for electrical nanopore recordings. In nanopore recordings, individual DNA strands are electrophoretically driven through a nanoscale pore leading to detectable blockades of ionic current. The tags cause characteristic electrical signatures in the current blockades of translocating DNA strands and thereby encode sequence information. The viability of the strategy is demonstrated by discriminating between drug resistance-conferring point mutations. By being independent of pore engineering, the new approach can potentially enhance the sensing repertoire of durable solid-state nanopores for which alternative sensing strategies developed for protein pores are not easily accessible.

Citation

Borsenberger, V., Mitchell, N. J., & Howorka, S. (2009). Chemically Labeled Nucleotides and Oligonucleotides Encode DNA for Sensing with Nanopores. Journal of the American Chemical Society, 131(22), 7530-7531. https://doi.org/10.1021/ja902004s

• Journal Article Type: Article
• Acceptance Date: Mar 14, 2009
• Online Publication Date: May 14, 2009
• Publication Date: Jun 10, 2009
• Deposit Date: Aug 31, 2020
• Journal: Journal of the American Chemical Society
• Print ISSN: 0002-7863
• Electronic ISSN: 1520-5126
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 131
• Issue: 22
• Pages: 7530-7531
• DOI: https://doi.org/10.1021/ja902004s
• Keywords: Colloid and Surface Chemistry; Biochemistry; General Chemistry; Catalysis
• Public URL: https://nottingham-repository.worktribe.com/output/4871595
• Publisher URL: https://pubs.acs.org/doi/10.1021/ja902004s