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Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential (2020)
Journal Article
Smith, S. J., Rowlinson, J., Estevez-Cebrero, M., Onion, D., Ritchie, A., Clarke, P., …Rahman, R. (2020). Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential. Neuro-Oncology Advances, 2(1), Article vdaa087. https://doi.org/10.1093/noajnl/vdaa087

Background Glioblastoma (GBM) is a highly aggressive brain tumor with rapid subclonal diversification, harboring molecular abnormalities that vary temporo-spatially, a contributor to therapy resistance. Fluorescence guided neurosurgical resection ut... Read More about Metabolism based isolation of invasive glioblastoma cells with specific gene signatures and tumorigenic potential.

Molecular Complexity of Lymphovascular Invasion: The Role of Cell Migration in Breast Cancer as a Prototype (2020)
Journal Article
Kariri, Y. A., Aleskandarany, M. A., Joseph, C., Toss, M. S., Kurozumi, S., Mohammed, O. J., …Rakha, E. A. (2020). Molecular Complexity of Lymphovascular Invasion: The Role of Cell Migration in Breast Cancer as a Prototype. Pathobiology, 87, 218–231. https://doi.org/10.1159/000508337

Lymphovascular invasion (LVI) is associated with poor outcome in breast cancer (BC); however, its underlying mechanisms remain ill-defined. LVI in BC develops through complex molecular pathways involving not only the interplay with the surrounding mi... Read More about Molecular Complexity of Lymphovascular Invasion: The Role of Cell Migration in Breast Cancer as a Prototype.

The natural alkaloid Jerantinine B has activity in acute myeloid leukemia cells through a mechanism involving c-Jun (2020)
Journal Article
Alhuthali, H. M., Bradshaw, T. D., Lim, K. H., Kam, T. S., & Seedhouse, C. H. (2020). The natural alkaloid Jerantinine B has activity in acute myeloid leukemia cells through a mechanism involving c-Jun. BMC Cancer, 20, Article 629. https://doi.org/10.1186/s12885-020-07119-2

© 2020 The Author(s). Background: Acute myeloid leukemia (AML) is a heterogenous hematological malignancy with poor long-term survival. New drugs which improve the outcome of AML patients are urgently required. In this work, the activity and mechanis... Read More about The natural alkaloid Jerantinine B has activity in acute myeloid leukemia cells through a mechanism involving c-Jun.

Visual histological assessment of morphological features reflects the underlying molecular profile in invasive breast cancer: a morphomolecular study (2020)
Journal Article
Alsaleem, M., Rakha, E. A., ElSharawy, K. A., Toss, M. S., Raafat, S., Mihai, R., …Mongan, N. P. (2020). Visual histological assessment of morphological features reflects the underlying molecular profile in invasive breast cancer: a morphomolecular study. Histopathology, 77(4), 631-645. https://doi.org/10.1111/his.14199

© 2020 The Authors. Histopathology published by John Wiley & Sons Ltd Aims: Tumour genotype and phenotype are related and can predict outcome. In this study, we hypothesised that the visual assessment of breast cancer (BC) morphological features ca... Read More about Visual histological assessment of morphological features reflects the underlying molecular profile in invasive breast cancer: a morphomolecular study.

Feasibility of integrated high?wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery (2020)
Journal Article
Liao, Z., Lizio, M. G., Corden, C., Khout, H., Rakha, E., & Notingher, I. (2020). Feasibility of integrated high?wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery. Journal of Raman Spectroscopy, 51(10), 1986-1995. https://doi.org/10.1002/jrs.5937

Intraoperative assessment of surgical margins remains one of the main challenges in cancer surgery. Raman spectroscopy can detect cancer cells with high accuracy, but it is time‐consuming. In this paper, we investigated a selective‐sampling Raman spe... Read More about Feasibility of integrated high?wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery.

Dopamine and cAMP?regulated phosphoprotein 32kDa (DARPP?32), protein phosphatase?1 and cyclin?dependent kinase 5 expression in ovarian cancer (2020)
Journal Article
Martin, S. G., Zhang, S., Yang, S., Saidy, B., Deen, S., & Storr, S. J. (2020). Dopamine and cAMP?regulated phosphoprotein 32kDa (DARPP?32), protein phosphatase?1 and cyclin?dependent kinase 5 expression in ovarian cancer. Journal of Cellular and Molecular Medicine, 24(16), 9165-9175. https://doi.org/10.1111/jcmm.15553

Dopamine and cyclic?AMP activated phosphoprotein Mr32kDa (DARPP?32) is a central signalling protein in neurotransmission. Following DARPP?32 phosphorylation by protein kinase A (PKA), DARPP?32 becomes a potent protein phosphatase 1 (PP1) inhibitor. D... Read More about Dopamine and cAMP?regulated phosphoprotein 32kDa (DARPP?32), protein phosphatase?1 and cyclin?dependent kinase 5 expression in ovarian cancer.

Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice (2020)
Journal Article
Raposo, T. P., Alfahed, A., Nateri, A. S., & Ilyas, M. (2020). Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice. International Journal of Experimental Pathology, 101(3-4), 80-86. https://doi.org/10.1111/iep.12352

ApcMin/+ mice are regarded as a standard animal model of colorectal cancer (CRC). Tensin4 (TNS4 or Cten) is a putative oncogene conferring features of stemness and promoting motility. Our objective was to assess TNS4 expression in intestinal adenomas... Read More about Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice.

Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement (2020)
Journal Article
Vankemmelbeke, M., McIntosh, R. S., Chua, J. X., Kirk, T., Daniels, I., Patsalidou, M., …Durrant, L. G. (2020). Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement. Cancer Research, 80(16), 3399-3412. https://doi.org/10.1158/0008-5472.CAN-19-3599

©2020 American Association for Cancer Research. Murine IgG3 glycan-targeting mAb often induces direct cell killing in the absence of immune effector cells or complement via a proinflammatory mechanism resembling oncotic necrosis. This cancer cell kil... Read More about Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement.

The prognostic significance of BMI1 expression in invasive breast cancer is dependent on its molecular subtypes (2020)
Journal Article
Althobiti, M., Muftah, A. A., Aleskandarany, M. A., Joseph, C., Toss, M. S., Green, A., & Rakha, E. (2020). The prognostic significance of BMI1 expression in invasive breast cancer is dependent on its molecular subtypes. Breast Cancer Research and Treatment, 182, 581–589. https://doi.org/10.1007/s10549-020-05719-x

Purpose: BMI-1, which is a major component of the polycomb group complex 1 is an essential epigenetic repressor of multiple regulatory genes and has been identified as a cancer stem cell (CSC) marker in several cancers. However, its role in breast c... Read More about The prognostic significance of BMI1 expression in invasive breast cancer is dependent on its molecular subtypes.

Non?invasive measurement of retinal permeability in a diabetic rat model (2020)
Journal Article
Whatmore, J. L., Allen, C. L., Malhi, N. K., Whatmore, J. D., Bates, D. O., & Arkill, K. P. (2020). Non?invasive measurement of retinal permeability in a diabetic rat model. Microcirculation, 27(6), https://doi.org/10.1111/micc.12623

Objective: The gold standard for measuring blood-retinal barrier permeability is the Evans blue assay. However, this technique has limitations in vivo, including non-specific tissue binding and toxicity. This study describes a non-toxic, high through... Read More about Non?invasive measurement of retinal permeability in a diabetic rat model.

Real?world tyrosine kinase inhibitor treatment pathways, monitoring patterns and responses in patients with chronic myeloid leukaemia in the United Kingdom: the UK TARGET CML study (2020)
Journal Article
Milojkovic, D., Cross, N. C., Ali, S., Byrne, J., Campbell, G., Dignan, F. L., …Mead, A. J. (2021). Real‐world tyrosine kinase inhibitor treatment pathways, monitoring patterns and responses in patients with chronic myeloid leukaemia in the United Kingdom: the UK TARGET CML study. British Journal of Haematology, 192(1), 62-74. https://doi.org/10.1111/bjh.16733

Management of chronic myeloid leukaemia (CML) has recently undergone dramatic changes, prompting the European LeukemiaNet (ELN) to issue recommendations in 2013; however, it remains unclear whether real-world CML management is consistent with these g... Read More about Real?world tyrosine kinase inhibitor treatment pathways, monitoring patterns and responses in patients with chronic myeloid leukaemia in the United Kingdom: the UK TARGET CML study.

Discovery of highly selective inhibitors of calmodulin-dependent kinases that restore insulin sensitivity in the diet-induced obese in vivo mouse model. (2020)
Journal Article
Fromont, C., Atzori, A., Kaur, D., Hashmi, L., Greco, G., Cabanillas, A., …Butterworth, S. (2020). Discovery of highly selective inhibitors of calmodulin-dependent kinases that restore insulin sensitivity in the diet-induced obese in vivo mouse model. Journal of Medicinal Chemistry, 63(13), 6784–6801. https://doi.org/10.1021/acs.jmedchem.9b01803

Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy (2020)
Journal Article
Grant, Z. L., Whitehead, L., Wong, V. H. Y., He, Z., Yan, R. Y., Miles, A. R., …Coultas, L. (2020). Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy. Journal of Clinical Investigation, 130(8), 4235-4251. https://doi.org/10.1172/jci127668

Aberrant, neovascular retinal blood vessel growth is a vision-threatening complication in ischemic retinal diseases. It is driven by retinal hypoxia frequently caused by capillary nonperfusion and endothelial cell (EC) loss. We investigated the role... Read More about Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy.

Blinded, multi-centre evaluation of drug-induced changes in contractility using human induced pluripotent stem cell-derived cardiomyocytes (2020)
Journal Article
Saleem, U., van Meer, B. J., Katili, P. A., Yusof, N. A. N. M., Mannhardt, I., Garcia, A. K., …Denning, C. (2020). Blinded, multi-centre evaluation of drug-induced changes in contractility using human induced pluripotent stem cell-derived cardiomyocytes. Toxicological Sciences, 176(1), 103–123. https://doi.org/10.1093/toxsci/kfaa058

Animal models are 78% accurate in determining whether drugs will alter contractility of the human heart. To evaluate the suitability of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for predictive safety pharmacology, we quan... Read More about Blinded, multi-centre evaluation of drug-induced changes in contractility using human induced pluripotent stem cell-derived cardiomyocytes.

Thioredoxin system protein expression is associated with poor clinical outcome in adult and paediatric gliomas and medulloblastomas (2020)
Journal Article
Martin, S., Yao, A., Storr, S. J., Al-Hadyan, K., Rahman, R., Smith, S., …Paine, S. (2020). Thioredoxin system protein expression is associated with poor clinical outcome in adult and paediatric gliomas and medulloblastomas. Molecular Neurobiology, 57(7), 2889–2901. https://doi.org/10.1007/s12035-020-01928-z

The thioredoxin (Trx) system is an important enzyme family that regulates cellular redox homeostasis. Protein expression of Trx system family members has been assessed in various cancers and linked to various clinicopathological variables, disease pr... Read More about Thioredoxin system protein expression is associated with poor clinical outcome in adult and paediatric gliomas and medulloblastomas.

PPFIA1 expression associates with poor response to endocrine treatment in luminal breast cancer (2020)
Journal Article
Alfarsi, L. H., El Ansari, R., Craze, M. L., Masisi, B. K., Ellis, I. O., Rakha, E. A., & Green, A. R. (2020). PPFIA1 expression associates with poor response to endocrine treatment in luminal breast cancer. BMC Cancer, 20(1), https://doi.org/10.1186/s12885-020-06939-6

Background PPFIA1 is an important regulator of cell migration and invasion, regulating focal adhesion signalling and disassembly. PPFIA1 is frequently amplified in breast cancer, and recent functional studies indicate that PPFIA1 is an important pro... Read More about PPFIA1 expression associates with poor response to endocrine treatment in luminal breast cancer.

A novel prognostic two-gene signature for triple negative breast cancer (2020)
Journal Article
Alsaleem, M. A., Ball, G., Toss, M. S., Raafat, S., Aleskandarany, M., Joseph, C., …Rakha, E. (2020). A novel prognostic two-gene signature for triple negative breast cancer. Modern Pathology, 33, 2208–2220. https://doi.org/10.1038/s41379-020-0563-7

The absence of a robust risk stratification tool for triple negative breast cancer (TNBC) underlies imprecise and non-selective treatment of these patients with cytotoxic chemotherapy. This study aimed to interrogate transcriptomes of TNBC resected s... Read More about A novel prognostic two-gene signature for triple negative breast cancer.

The Use of Biomaterials in Internal Radiation Therapy (2020)
Journal Article
Milborne, B., Arafat, A., Layfield, R., Thompson, A., & Ahmed, I. (2020). The Use of Biomaterials in Internal Radiation Therapy. Recent Progress in Materials, 2(2), https://doi.org/10.21926/rpm.2002012

Radiotherapy has become one of the most prominent and effective modalities for cancer treatment and care. Ionising radiation, delivered either from external or internal sources, can be targeted to cancerous cells causing damage to DNA that can induce... Read More about The Use of Biomaterials in Internal Radiation Therapy.

Investigating TNS4 in the Colorectal Tumor Microenvironment Using 3D Spheroid Models of Invasion (2020)
Journal Article
Raposo, T. P., Susanti, S., & Ilyas, M. (2020). Investigating TNS4 in the Colorectal Tumor Microenvironment Using 3D Spheroid Models of Invasion. Advanced Biosystems, 4(6), https://doi.org/10.1002/adbi.202000031

TNS4 (Tensin 4 or Cten) is a putative oncogene in colorectal cancer (CRC) with a role in regulating cell adhesion, motility, invasion, and epithelial to mesenchymal transition (EMT). The objective is to study the role of TNS4 in CRC using more realis... Read More about Investigating TNS4 in the Colorectal Tumor Microenvironment Using 3D Spheroid Models of Invasion.