All Outputs (214)

Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages (2019)
Journal Article
Wall, R. J., Zeeshan, M., Katris, N. J., Limenitakis, R., Rea, E., Stock, J., …Tewari, R. (2019). Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages. Cellular Microbiology, 21(10), Article e13082. https://doi.org/10.1111/cmi.13082

The myosin superfamily comprises of actin‐dependent eukaryotic molecular motors important in a variety of cellular functions. Although well studied in many systems, knowledge of their functions in Plasmodium, the causative agent of malaria, is restri... Read More about Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages.

Efficacy of direct‐acting antivirals: UK real‐world data from a well‐characterised predominantly cirrhotic HCV cohort (2019)
Journal Article
Macken, L., Gelson, W., Priest, M., Abouda, G., Barclay, S., Fraser, A., …Verma, S. (2019). Efficacy of direct‐acting antivirals: UK real‐world data from a well‐characterised predominantly cirrhotic HCV cohort. Journal of Medical Virology, 91(11), 1979-1988. https://doi.org/10.1002/jmv.25552

Direct-acting antivirals (DAAs) have revolutionised the management of chronic hepatitis C virus (HCV) infection. We describe UK real-world DAA experience. Individuals commencing HCV treatment containing a DAA regimen (Mar 2014-Nov 2016), participatin... Read More about Efficacy of direct‐acting antivirals: UK real‐world data from a well‐characterised predominantly cirrhotic HCV cohort.

Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review (2019)
Journal Article
Tarbiah, N., Todd, I., Tighe, P. J., & Fairclough, L. C. (2019). Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review. Basic and Clinical Pharmacology and Toxicology, 125(5), 474-483. https://doi.org/10.1111/bcpt.13278

The aim of the present study was to compare concentrations of IgG, IgA, IgM and IgD in both serum and saliva samples from smoking and non-smoking subjects using a protein microarray assay. The findings were also compared to previous studies. Serum an... Read More about Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review.

Differential Stimulation of Post-Exercise Myofibrillar Protein Synthesis in Humans Following Isonitrogenous, Isocaloric Pre-Exercise Feeding (2019)
Journal Article
Davies, R. W., Bass, J. J., Carson, B. P., Norton, C., Kozior, M., Amigo-Benavent, M., …Jakeman, P. M. (2019). Differential Stimulation of Post-Exercise Myofibrillar Protein Synthesis in Humans Following Isonitrogenous, Isocaloric Pre-Exercise Feeding. Nutrients, 11(7), Article 1657. https://doi.org/10.3390/nu11071657

The aim of this study was to test the effects of two disparate isonitrogenous, isocaloric pre-exercise feeds on deuterium-oxide (D2O) derived measures of myofibrillar protein synthesis (myoPS) in humans. Methods: In a double-blind parallel group desi... Read More about Differential Stimulation of Post-Exercise Myofibrillar Protein Synthesis in Humans Following Isonitrogenous, Isocaloric Pre-Exercise Feeding.

Simulations of the effect of diffusion on asymmetric spin echo based quantitative BOLD: An investigation of the origin of deoxygenated blood volume overestimation (2019)
Journal Article
Stone, A. J., Holland, N. C., Berman, A. J., & Blockley, N. P. (2019). Simulations of the effect of diffusion on asymmetric spin echo based quantitative BOLD: An investigation of the origin of deoxygenated blood volume overestimation. NeuroImage, 201, Article 116035. https://doi.org/10.1016/j.neuroimage.2019.116035

Quantitative BOLD (qBOLD) is a technique for mapping oxygen extraction fraction (OEF) and deoxygenated blood volume (DBV) in the human brain. Recent measurements using an asymmetric spin echo (ASE) based qBOLD approach produced estimates of DBV which... Read More about Simulations of the effect of diffusion on asymmetric spin echo based quantitative BOLD: An investigation of the origin of deoxygenated blood volume overestimation.

Prevalence and predictors of polypharmacy prescription among type 2 diabetes patients at a tertiary care department in Ningbo, China: A retrospective database study (2019)
Journal Article
Li, J., Chattopadhyay, K., Xu, M., Chen, Y., Hu, F., Wang, X., & Li, L. (2019). Prevalence and predictors of polypharmacy prescription among type 2 diabetes patients at a tertiary care department in Ningbo, China: A retrospective database study. PLoS ONE, 14(7), Article e0220047. https://doi.org/10.1371/journal.pone.0220047

Objectives To determine the prevalence of polypharmacy prescription among type 2 diabetes (T2DM) patients at a tertiary care department in Ningbo, China, and to determine factors that independently predict this polypharmacy prescription. Methods... Read More about Prevalence and predictors of polypharmacy prescription among type 2 diabetes patients at a tertiary care department in Ningbo, China: A retrospective database study.

Phosphorylation and functionality of CdtR in Clostridium difficile (2019)
Journal Article
Bilverstone, T., Minton, N., & Kuehne, S. (2019). Phosphorylation and functionality of CdtR in Clostridium difficile. Anaerobe, 58, 103-109. https://doi.org/10.1016/j.anaerobe.2019.102074

The production of TcdA, TcdB and CDT in Clostridium difficile PCR ribotype 027, is regulated by the two-component system response regulator CdtR. Despite this, little is known about the signal transduction pathway leading to the activation of CdtR. I... Read More about Phosphorylation and functionality of CdtR in Clostridium difficile.

Genome Analysis Reveals Genetic Admixture and Signature of Selection for Productivity and Environmental Traits in Iraqi Cattle (2019)
Journal Article
Alshawi, A., Essa, A., Al-Bayatti, S., & Hanotte, O. (2019). Genome Analysis Reveals Genetic Admixture and Signature of Selection for Productivity and Environmental Traits in Iraqi Cattle. Frontiers in Genetics, 10, Article 609. https://doi.org/10.3389/fgene.2019.00609

The Near East cattle are adapted to different agro-ecological zones including desert areas, mountains habitats, and humid regions along the Tigris and Euphrates rivers system. The region was one of the earliest and most significant areas of cattle hu... Read More about Genome Analysis Reveals Genetic Admixture and Signature of Selection for Productivity and Environmental Traits in Iraqi Cattle.

Quality of life in adults with muscular dystrophy (2019)
Journal Article
Jacques, M. F., Stockley, R. C., Onambele-Pearson, G. L., Reeves, N. D., Stebbings, G. K., Dawson, E. A., …Morse, C. I. (2019). Quality of life in adults with muscular dystrophy. Health and Quality of Life Outcomes, 17, Article 121. https://doi.org/10.1186/s12955-019-1177-y

Background: Muscle weakness is a defining characteristic of Muscular Dystrophy (MD); however, yet while speculated, objective measures of muscle weakness has not been reported in relation to quality of life in adults with MD. Objectives: 1) compare t... Read More about Quality of life in adults with muscular dystrophy.

Admixture between ancient lineages, selection, and the formation of sympatric stickleback species-pairs (2019)
Journal Article
Dean, L. L., Magalhaes, I. S., Foote, A., D’Agostino, D., McGowan, S., & MacColl, A. D. (2019). Admixture between ancient lineages, selection, and the formation of sympatric stickleback species-pairs. Molecular Biology and Evolution, 36(11), 2481-2497. https://doi.org/10.1093/molbev/msz161

Ecological speciation has become a popular model for the development and maintenance of reproductive isolation in closely related sympatric pairs of species or ecotypes. An implicit assumption has been that such pairs originate (possibly with gene fl... Read More about Admixture between ancient lineages, selection, and the formation of sympatric stickleback species-pairs.

Investigating the Influence of Tracer Kinetics on Competition-Kinetic Association Binding Assays: Identifying the Optimal Conditions for Assessing the Kinetics of Low-Affinity Compounds (2019)
Journal Article
Sykes, D. A., Jain, P., & Charlton, S. J. (2019). Investigating the Influence of Tracer Kinetics on Competition-Kinetic Association Binding Assays: Identifying the Optimal Conditions for Assessing the Kinetics of Low-Affinity Compounds. Molecular Pharmacology, 96(3), 378-392. https://doi.org/10.1124/mol.119.116764

An increased appreciation of the importance of optimizing drug-binding kinetics has lead to the development of various techniques for measuring the kinetics of unlabeled compounds. One approach is the competition-association kinetic binding method fi... Read More about Investigating the Influence of Tracer Kinetics on Competition-Kinetic Association Binding Assays: Identifying the Optimal Conditions for Assessing the Kinetics of Low-Affinity Compounds.

Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells (2019)
Journal Article
De Melo, N., McGinlay, S., Markus, R., Macri-Pellizzeri, L., Symonds, M. E., Ahmed, I., & Sottile, V. (2019). Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics, 4(3), 1-10. https://doi.org/10.3390/biomimetics4030048

Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existing in bone marrow. When isolated in vitro, these stem cells recapitulate osteoblast or adipocyte formation if treated... Read More about Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.

Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-β peptide in Alzheimer's disease (2019)
Journal Article
Rosini, M., Simoni, E., Caporaso, R., Basagni, F., Catanzaro, M., Abu, I. F., …Minarini, A. (2019). Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-β peptide in Alzheimer's disease. European Journal of Medicinal Chemistry, 180, 111-120. https://doi.org/10.1016/j.ejmech.2019.07.011

N-methyl-d-aspartate receptors (NMDAR) are critically involved in the pathogenesis of Alzheimer's disease (AD). Acting as an open-channel blocker, the anti-AD drug memantine preferentially targets NMDAR overactivation, which has been proposed to trig... Read More about Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-β peptide in Alzheimer's disease.

Fecal microbiota transplants: emerging social representations in the English-language print media (2019)
Journal Article
McLeod, C., Nerlich, B., & Jaspal, R. (2019). Fecal microbiota transplants: emerging social representations in the English-language print media. New Genetics and Society, 38(3), 331-351. https://doi.org/10.1080/14636778.2019.1637721

This study investigates how English-language news sources have represented fecal microbiota transplants (FMT). FMT involves transferring stool from a healthy donor to a recipient with a dysfunctional intestinal flora in order to repopulate their gut... Read More about Fecal microbiota transplants: emerging social representations in the English-language print media.

Culturing and Mating of Aspergillus fumigatus (2019)
Journal Article
Ashton, G. D., & Dyer, P. S. (2019). Culturing and Mating of Aspergillus fumigatus. Current Protocols in Microbiology, 54(1), Article e87. https://doi.org/10.1002/cpmc.87

Aspergillus fumigatus is an opportunistic human fungal pathogen, capable of causing invasive aspergillosis in patients with compromised immune systems. The fungus was long considered a purely asexual organism. However, a sexual cycle was reported in... Read More about Culturing and Mating of Aspergillus fumigatus.

A novel conjugal donor strain for improved DNA transfer into Clostridium spp. (2019)
Journal Article
Woods, C., Humphreys, C. M., Rodrigues, R. M., Ingle, P., Rowe, P., Henstra, A. M., …Minton, N. P. (2019). A novel conjugal donor strain for improved DNA transfer into Clostridium spp. Anaerobe, 59, 184-191. https://doi.org/10.1016/j.anaerobe.2019.06.020

© 2019 The Authors Importance: The ability to transfer genetic material into a target organism is crucial for the development of a wide range of targeted genetic manipulation techniques. Overcoming the organisms’ native restriction systems which targ... Read More about A novel conjugal donor strain for improved DNA transfer into Clostridium spp..

A Hybrid Evolutionary Strategy to Optimise Early-Stage Cancer Screening (2019)
Presentation / Conference Contribution
Figueredo, G. P., Shi, P., Parkes, A. J., Evans, K., Garibaldi, J. M., Negm, O., …Robertson, J. (2019). A Hybrid Evolutionary Strategy to Optimise Early-Stage Cancer Screening. In 2019 IEEE Congress on Evolutionary Computation (CEC) (95-102). https://doi.org/10.1109/CEC.2019.8790316

Current methods to identify cutoff values for tumour-associated molecules (antigens) discrimination are based on statistics and brute force. These methods applied to cancer screening problems are very inefficient, especially with large data sets with... Read More about A Hybrid Evolutionary Strategy to Optimise Early-Stage Cancer Screening.

Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD (2019)
Journal Article
Latimer, L., Constantin, D., Greening, N., Calvert, L., Menon, M., & Greenhaff, P. (2019). Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD. International Journal of Chronic Obstructive Pulmonary Disease, 14, 1355-1364. https://doi.org/10.2147/COPD.S189896

Background: Voluntary resistance exercise (RE) training increases muscle mass and strength in patients with chronic obstructive pulmonary disease (COPD). Nonvolitional transcutaneous neuromuscular electrical stimulation (NMES) may be an alternative s... Read More about Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD.

Targeted DNA transposition in vitro using a dCas9-transposase fusion protein (2019)
Journal Article
Bhatt, S., & Chalmers, R. (2019). Targeted DNA transposition in vitro using a dCas9-transposase fusion protein. Nucleic Acids Research, 47(15), 8126–8135. https://doi.org/10.1093/nar/gkz552

Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates effic... Read More about Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.

Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth (2019)
Journal Article
Loczenski-Brown, D. M., Jones, S., Luckett, J., Daniel, Z., Brearley, M. C., Ebling, F. J. P., …Brameld, J. M. (2019). Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth. PLoS ONE, 14(6), 1-12. https://doi.org/10.1371/journal.pone.0218970

We previously identified PEPCK-M (encoded by the Pck2 gene) to be highly up-regulated in skeletal muscle of pigs treated with Ractopamine, an anabolic beta-adrenergic receptor agonist. To determine whether PEPCK-M had a causative role in modulating t... Read More about Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth.