A novel conjugal donor strain for improved DNA transfer into Clostridium spp.

Woods, Craig; Humphreys, Christopher M.; Rodrigues, Raquel Mesquita; Ingle, Patrick; Rowe, Peter; Henstra, Anne M.; Köpke, Michael; Simpson, Sean D.; Winzer, Klaus; Minton, Nigel P.

Abstract

© 2019 The Authors Importance: The ability to transfer genetic material into a target organism is crucial for the development of a wide range of targeted genetic manipulation techniques. Overcoming the organisms’ native restriction systems which target foreign incoming DNA is one strategy that can increase the efficiency of genetic transfer. The novel E. coli donor strain described here employs this strategy, increasing the frequencies of conjugation into a range of clostridial strains, and therefore opening up the potential to implement novel gene manipulation techniques. Furthermore this novel donor strain has potential applications across a wide range of genetically recalcitrant organisms, and should be beneficial wherever the frequently occurring Type IV restriction systems are possessed by the target in question.

Citation

Woods, C., Humphreys, C. M., Rodrigues, R. M., Ingle, P., Rowe, P., Henstra, A. M., …Minton, N. P. (2019). A novel conjugal donor strain for improved DNA transfer into Clostridium spp. Anaerobe, 59, 184-191. https://doi.org/10.1016/j.anaerobe.2019.06.020

• Journal Article Type: Article
• Acceptance Date: Jun 29, 2019
• Online Publication Date: Jun 30, 2019
• Publication Date: Oct 1, 2019
• Deposit Date: Jul 9, 2019
• Publicly Available Date: Aug 27, 2019
• Journal: Anaerobe
• Print ISSN: 1075-9964
• Electronic ISSN: 1095-8274
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 59
• Pages: 184-191
• DOI: https://doi.org/10.1016/j.anaerobe.2019.06.020
• Keywords: Clostridium; DNA transfer; conjugatio; nrestriction-methylation systems; C. difficile; C. sporogenes; C. autoethanogenum
• Public URL: https://nottingham-repository.worktribe.com/output/2290347
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1075996419301179?via%3Dihub
• Contract Date: Jul 9, 2019