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Culturing and Mating of Aspergillus fumigatus

Ashton, George D.; Dyer, Paul S.


George D. Ashton

Professor of Fungal Biology


Aspergillus fumigatus is an opportunistic human fungal pathogen, capable of causing invasive aspergillosis in patients with compromised immune systems. The fungus was long considered a purely asexual organism. However, a sexual cycle was reported in 2009, with methods described to induce mating under laboratory conditions. The presence of a sexual cycle now offers a valuable tool for classical genetic analysis of the fungus, such as allowing determination of whether traits of interest are mono- or poly-genic in nature. For example, the sexual cycle is currently being exploited to determine the genetic basis of traits of medical importance such as resistance to azole antifungals and virulence, and to characterise the genes involved. The sexual cycle can also be used to assess the possibility of gene flow between isolates.

This unit describes protocols for culturing of A. fumigatus and for inducing sexual reproduction between compatible MAT1-1 and MAT1-2 isolates of the species. The unit also provides working methods for harvesting sexual structures, isolating single-spore progeny and confirming whether sexual recombination has occurred.


Ashton, G. D., & Dyer, P. S. (2019). Culturing and Mating of Aspergillus fumigatus. Current Protocols in Microbiology, 54(1),

Journal Article Type Article
Acceptance Date May 24, 2019
Online Publication Date Jul 1, 2019
Publication Date Sep 1, 2019
Deposit Date Jun 3, 2019
Publicly Available Date Jul 2, 2020
Journal Current Protocols in Microbiology
Print ISSN 1934-8525
Electronic ISSN 1934-8533
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 54
Issue 1
Article Number e87
Keywords Microbiology; Parasitology; Virology; General Medicine
Public URL
Publisher URL
Additional Information Published: 2019-07-01


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