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Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD

Latimer, Lorna; Constantin, Despina; Greening, Neil; Calvert, Lori; Menon, Manoj; Greenhaff, Paul

Authors

Lorna Latimer

Neil Greening

Lori Calvert

Manoj Menon



Abstract

Background: Voluntary resistance exercise (RE) training increases muscle mass and strength in patients with chronic obstructive pulmonary disease (COPD). Nonvolitional transcutaneous neuromuscular electrical stimulation (NMES) may be an alternative strategy for reducing ambulatory muscle weakness in patients unable to perform RE training, but little comparative data are available. This study, therefore, investigated changes in muscle mRNA abundance of a number of gene targets in response to a single bout of NMES compared with RE.
Methods: Twenty-six patients with stable COPD (15 male; FEV1, 43±18% predicted; age, 64±8 years; fat free mass index, 16.6±1.8 kg/m2,) undertook 30 minutes of quadriceps NMES (50 Hz, current at the limit of tolerance) or 5×30 maximal voluntary isokinetic knee extensions. Vastus lateralis muscle biopsies were obtained at rest immediately before and 24 hours after intervention. Expression of 384 targeted mRNA transcripts was assessed by real time TaqMan PCR. Significant change in expression from baseline was determined using the ΔΔCT method with a false discovery rate (FDR) of

Journal Article Type Article
Publication Date Jun 28, 2019
Journal International Journal of Chronic Obstructive Pulmonary Disease
Electronic ISSN 1178-2005
Publisher Dove Medical Press
Peer Reviewed Peer Reviewed
Volume 14
Pages 1355-1364
APA6 Citation Latimer, L., Constantin, D., Greening, N., Calvert, L., Menon, M., & Greenhaff, P. (2019). Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD. International Journal of Chronic Obstructive Pulmonary Disease, 14, 1355-1364. https://doi.org/10.2147/COPD.S189896
DOI https://doi.org/10.2147/COPD.S189896
Keywords COPD; NMES; skeletal muscle; gene expression; resistance exercise
Publisher URL https://www.dovepress.com/impact-of-transcutaneous-neuromuscular-electrical-stimulation-or-resis-peer-reviewed-article-COPD

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