Differential Stimulation of Post-Exercise Myofibrillar Protein Synthesis in Humans Following Isonitrogenous, Isocaloric Pre-Exercise Feeding

Davies, Robert W.; Bass, Joseph J.; Carson, Brian P.; Norton, Catherine; Kozior, Marta; Amigo-Benavent, Miryam; Wilkinson, Daniel J.; Brook, Matthew S.; Atherton, Philip J.; Smith, Kenneth; Jakeman, Philip M.

Abstract

The aim of this study was to test the effects of two disparate isonitrogenous, isocaloric pre-exercise feeds on deuterium-oxide (D2O) derived measures of myofibrillar protein synthesis (myoPS) in humans. Methods: In a double-blind parallel group design, 22 resistance-trained men aged 18 to 35 years ingested a meal (6 kcal·kg−1, 0.8 g·kg−1 carbohydrate, 0.2 g·kg−1 fat) with 0.33 g·kg−1 nonessential amino acids blend (NEAA) or whey protein (WHEY), prior to resistance exercise (70% 1RM back-squats, 10 reps per set to failure, 25% duty cycle). Biopsies of M. vastus lateralis were obtained pre-ingestion (PRE) and +3 h post-exercise (POST). The myofibrillar fractional synthetic rate (myoFSR) was calculated via deuterium labelling of myofibrillar-bound alanine, measured by gas chromatography–pyrolysis–isotope ratio mass spectrometry (GC-Pyr-IRMS). Data are a mean percentage change (95% CI). Results: There was no discernable change in myoFSR following NEAA (10(−5, 25) %, p = 0.235), whereas an increase in myoFSR was observed after WHEY (28 (13, 43) %, p = 0.003). Conclusions: Measured by a D2O tracer technique, a disparate myoPS response was observed between NEAA and WHEY. Pre-exercise ingestion of whey protein increased post-exercise myoPS, whereas a NEAA blend did not, supporting the use of NEAA as a viable isonitrogenous negative control.

Citation

Davies, R. W., Bass, J. J., Carson, B. P., Norton, C., Kozior, M., Amigo-Benavent, M., Wilkinson, D. J., Brook, M. S., Atherton, P. J., Smith, K., & Jakeman, P. M. (2019). Differential Stimulation of Post-Exercise Myofibrillar Protein Synthesis in Humans Following Isonitrogenous, Isocaloric Pre-Exercise Feeding. Nutrients, 11(7), Article 1657. https://doi.org/10.3390/nu11071657

• Journal Article Type: Article
• Acceptance Date: Jul 13, 2019
• Online Publication Date: Jul 19, 2019
• Publication Date: Jul 19, 2019
• Deposit Date: Aug 20, 2019
• Publicly Available Date: Aug 20, 2019
• Journal: Nutrients
• Electronic ISSN: 2072-6643
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 7
• Article Number: 1657
• DOI: https://doi.org/10.3390/nu11071657
• Keywords: Food Science
• Public URL: https://nottingham-repository.worktribe.com/output/2451115
• Publisher URL: https://www.mdpi.com/2072-6643/11/7/1657
• Contract Date: Aug 20, 2019