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Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages

Wall, Richard J.; Zeeshan, Mohammad; Katris, Nicholas J.; Limenitakis, Rebecca; Rea, Edward; Stock, Jessica; Brady, Declan; Waller, Ross F.; Holder, Anthony A.; Tewari, Rita

Authors

Richard J. Wall

Nicholas J. Katris

Rebecca Limenitakis

Edward Rea

Jessica Stock

Declan Brady

Ross F. Waller

Anthony A. Holder

RITA TEWARI RITA.TEWARI@NOTTINGHAM.AC.UK
Professor of Parasite Cell Biology



Abstract

The myosin superfamily comprises of actin‐dependent eukaryotic molecular motors important in a variety of cellular functions. Although well studied in many systems, knowledge of their functions in Plasmodium, the causative agent of malaria, is restricted. Previously, six myosins were identified in this genus, including three Class XIV myosins found only in Apicomplexa and some Ciliates. The well characterized MyoA is a Class XIV myosin essential for gliding motility and invasion. Here, we characterize all other Plasmodium myosins throughout the parasite life cycle and show that they have very diverse patterns of expression and cellular location. MyoB and MyoE, the other two Class XIV myosins, are expressed in all invasive stages, with apical and basal locations, respectively. Gene deletion revealed that MyoE is involved in sporozoite traversal, MyoF and MyoK are likely essential in the asexual blood stages, and MyoJ and MyoB are not essential. Both MyoB and its essential light chain (MCL‐B) are localised at the apical end of ookinetes but expressed at completely different time points. This work provides a better understanding of the role of actomyosin motors in Apicomplexan parasites, particularly in the motile and invasive stages of Plasmodium during sexual and asexual development within the mosquito.

Citation

Wall, R. J., Zeeshan, M., Katris, N. J., Limenitakis, R., Rea, E., Stock, J., …Tewari, R. (2019). Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages. Cellular Microbiology, 21(10), Article e13082. https://doi.org/10.1111/cmi.13082

Journal Article Type Article
Acceptance Date Jul 3, 2019
Online Publication Date Jul 23, 2019
Publication Date Oct 1, 2019
Deposit Date Aug 6, 2019
Publicly Available Date Mar 28, 2024
Journal Cellular Microbiology
Print ISSN 1462-5814
Electronic ISSN 1462-5822
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 21
Issue 10
Article Number e13082
DOI https://doi.org/10.1111/cmi.13082
Keywords Immunology; Microbiology; Virology
Public URL https://nottingham-repository.worktribe.com/output/2395102
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13082

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