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All Outputs (169)

Hepatitis C subtyping assay failure in UK patients born in sub-Saharan Africa: Implications for global treatment and elimination (2022)
Journal Article
Adeboyejo, K., King, B., Tsoleridis, T., Tarr, A. W., McLauchlan, J., Irving, W. L., …McClure, C. P. (2023). Hepatitis C subtyping assay failure in UK patients born in sub-Saharan Africa: Implications for global treatment and elimination. Journal of Medical Virology, 95(1), Article e28178. https://doi.org/10.1002/jmv.28178

Background andAims: The newly developed direct-acting antivirals have revolutionized the treatment of chronic hepatitis C virus (HCV), with cure rates as high as 98% in some cohorts. Although genome sequencing has demonstrated that some subtypes of H... Read More about Hepatitis C subtyping assay failure in UK patients born in sub-Saharan Africa: Implications for global treatment and elimination.

Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor (2022)
Journal Article
Asghar, J., Latif, L., Alexander, S. P. H., & Kendall, D. A. (2022). Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor. Frontiers in Pharmacology, 13, Article 933356. https://doi.org/10.3389/fphar.2022.933356

Background: Extracellular signal-regulated kinases (ERKs) are important signaling mediators in mammalian cells and, as a result, one of the major areas of research focus. The detection and quantification of ERK phosphorylation as an index of activati... Read More about Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor.

Redox stress and metal dys-homeostasis appear as hallmarks of early prion disease pathogenesis in mice (2022)
Journal Article
Spiers, J. G., Cortina Chen, H. J., Barry, T. L., Bourgognon, J. M., & Steinert, J. R. (2022). Redox stress and metal dys-homeostasis appear as hallmarks of early prion disease pathogenesis in mice. Free Radical Biology and Medicine, 192, 182-190. https://doi.org/10.1016/j.freeradbiomed.2022.09.025

Neurodegenerative diseases are associated with a multitude of dysfunctional cellular pathways. One major contributory factor is a redox stress challenge during the development of several protein misfolding conditions including Alzheimer's (AD), Parki... Read More about Redox stress and metal dys-homeostasis appear as hallmarks of early prion disease pathogenesis in mice.

Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space (2022)
Journal Article
Okoye, J. C., Bellamy-Carter, J., Oldham, N. J., Oldfield, N. J., Mahdavi, J., & Soultanas, P. (2022). Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space. Computational and Structural Biotechnology Journal, 20, 5355-5363. https://doi.org/10.1016/j.csbj.2022.09.032

Ferric chelates like ferric tyrosinate (TYPLEX) and the closely related ferric quinate (QPLEX) are structural mimics of bacterial siderophores. TYPLEX has been trialled as a feed additive in farming of commercial broilers, reducing Campylobacter load... Read More about Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space.

DNA methylation signatures of Alzheimer’s disease neuropathology in the cortex are primarily driven by variation in non-neuronal cell-types (2022)
Journal Article
Shireby, G., Dempster, E. L., Policicchio, S., Smith, R. G., Pishva, E., Chioza, B., …Mill, J. (2022). DNA methylation signatures of Alzheimer’s disease neuropathology in the cortex are primarily driven by variation in non-neuronal cell-types. Nature Communications, 13, Article 5620. https://doi.org/10.1038/s41467-022-33394-7

Alzheimer’s disease (AD) is a chronic neurodegenerative disease characterized by the progressive accumulation of amyloid-beta and neurofibrillary tangles of tau in the neocortex. We profiled DNA methylation in two regions of the cortex from 631 donor... Read More about DNA methylation signatures of Alzheimer’s disease neuropathology in the cortex are primarily driven by variation in non-neuronal cell-types.

Dissecting microbial communities and resistomes for interconnected humans, soil, and livestock (2022)
Journal Article
Maciel-Guerra, A., Baker, M., Hu, Y., Wang, W., Zhang, X., Rong, J., …Dottorini, T. (2023). Dissecting microbial communities and resistomes for interconnected humans, soil, and livestock. ISME Journal, 17, 21-35. https://doi.org/10.1038/s41396-022-01315-7

A debate is currently ongoing as to whether intensive livestock farms may constitute reservoirs of clinically relevant antimicrobial resistance (AMR), thus posing a threat to surrounding communities. Here, combining shotgun metagenome sequencing, mac... Read More about Dissecting microbial communities and resistomes for interconnected humans, soil, and livestock.

Optical control of the β2-adrenergic receptor with opto-prop-2: A cis-active azobenzene analog of propranolol (2022)
Journal Article
Bosma, R., Dijon, N. C., Zheng, Y., Schihada, H., Hauwert, N. J., Shi, S., …Leurs, R. (2022). Optical control of the β2-adrenergic receptor with opto-prop-2: A cis-active azobenzene analog of propranolol. iScience, 25(9), Article 104882. https://doi.org/10.1016/j.isci.2022.104882

In this study, we synthesized and evaluated new photoswitchable ligands for the beta-adrenergic receptors β1-AR and β2-AR, applying an azologization strategy to the first-generation beta-blocker propranolol. The resulting compounds (Opto-prop-1, -2,... Read More about Optical control of the β2-adrenergic receptor with opto-prop-2: A cis-active azobenzene analog of propranolol.

The signaling and selectivity of α-adrenoceptor agonists for the human α2A, α2B and α2C-adrenoceptors and comparison with human α1 and β-adrenoceptors (2022)
Journal Article
Proudman, R. G. W., Akinaga, J., & Baker, J. G. (2022). The signaling and selectivity of α-adrenoceptor agonists for the human α2A, α2B and α2C-adrenoceptors and comparison with human α1 and β-adrenoceptors. Pharmacology Research and Perspectives, 10(5), Article e01003. https://doi.org/10.1002/prp2.1003

α2-adrenoceptors, (α2A, α2B and α2C-subtypes), are Gi-coupled receptors. Central activation of brain α2A and α2C-adrenoceptors is the main site for α2-agonist mediated clinical responses in hypertension, ADHD, muscle spasm and ITU management of sedat... Read More about The signaling and selectivity of α-adrenoceptor agonists for the human α2A, α2B and α2C-adrenoceptors and comparison with human α1 and β-adrenoceptors.

Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice (2022)
Journal Article
Morgan, H. L., Furse, S., Dias, I. H. K., Shabir, K., Castellanos, M., Khan, I., …Watkins, A. J. (2022). Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice. Communications Biology, 5(1), Article 929. https://doi.org/10.1038/s42003-022-03914-8

The underlying mechanisms driving paternally-programmed metabolic disease in offspring remain poorly defined. We fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a m... Read More about Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice.

Fine-scale geographical sampling and molecular characterization of the giant African land snail in its invasive range in Asia shows low genetic diversity, new haplotypes and the emergence of another haplotype from the Indian Ocean Islands (2022)
Journal Article
Vijayan, K., Suganthasakthivel, R., Naggs, F., Fontanilla, I. K., Soorae, P. S., Sajeev, T. V., & Wade, C. M. (2022). Fine-scale geographical sampling and molecular characterization of the giant African land snail in its invasive range in Asia shows low genetic diversity, new haplotypes and the emergence of another haplotype from the Indian Ocean Islands. Biological Journal of the Linnean Society, 137(3), 421-433. https://doi.org/10.1093/biolinnean/blac106

Native to East Africa, the giant African snail Lissachatina [=Achatina] fulica (Bowdich, 1822) is a tropical crop pest and one of the world’s top 100 invasive species. It is now present in at least 52 countries worldwide, with an actively expanding r... Read More about Fine-scale geographical sampling and molecular characterization of the giant African land snail in its invasive range in Asia shows low genetic diversity, new haplotypes and the emergence of another haplotype from the Indian Ocean Islands.

Influence of Eimeria maxima coccidia infection on gut microbiome diversity and composition of the jejunum and cecum of indigenous chicken (2022)
Journal Article
Jebessa, E., Guo, L., Chen, X., Bello, S. F., Cai, B., Girma, M., …Nie, Q. (2022). Influence of Eimeria maxima coccidia infection on gut microbiome diversity and composition of the jejunum and cecum of indigenous chicken. Frontiers in Immunology, 13, Article 994224. https://doi.org/10.3389/fimmu.2022.994224

Coccidiosis is an economically significant protozoan disease and an intracellular parasite that significantly impacts poultry production. The gastrointestinal tract microbiota plays a central role in host health and metabolism, and these microbes enh... Read More about Influence of Eimeria maxima coccidia infection on gut microbiome diversity and composition of the jejunum and cecum of indigenous chicken.

Human adaptation to immobilization: Novel insights of impacts on glucose disposal and fuel utilization (2022)
Journal Article
Shur, N. F., Simpson, E. J., Crossland, H., Chivaka, P. K., Constantin, D., Cordon, S. M., …Greenhaff, P. L. (2022). Human adaptation to immobilization: Novel insights of impacts on glucose disposal and fuel utilization. Journal of Cachexia, Sarcopenia and Muscle, 13(6), 2999-3013. https://doi.org/10.1002/jcsm.13075

Background: Bed rest (BR) reduces whole-body insulin-stimulated glucose disposal (GD) and alters muscle fuel metabolism, but little is known about metabolic adaptation from acute to chronic BR nor the mechanisms involved, particularly when volunteers... Read More about Human adaptation to immobilization: Novel insights of impacts on glucose disposal and fuel utilization.

A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin (2022)
Journal Article
Belsham, H. R., Alghamdi, H. M., Dave, N., Rathbone, A. J., Wickstead, B., & Friel, C. T. (2022). A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin. Open Biology, 12(8), Article 220133. https://doi.org/10.1098/rsob.220133

The activity of a kinesin is largely determined by the approximately 350 residue motor domain, and this region alone is sufficient to classify a kinesin as a member of a particular family. The kinesin-13 family are a group of microtubule depolymerizi... Read More about A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin.

Aberrant expression of miR-133a in endothelial cells inhibits angiogenesis by reducing pro-angiogenic but increasing anti-angiogenic gene expression (2022)
Journal Article
Ahmed, S., Kurusamy, S., David, E. L. S., Khan, K., Kalyanakrishnan, K., Ian-Gobo, M., …Armesilla, A. L. (2022). Aberrant expression of miR-133a in endothelial cells inhibits angiogenesis by reducing pro-angiogenic but increasing anti-angiogenic gene expression. Scientific Reports, 12(1), Article 14730. https://doi.org/10.1038/s41598-022-19172-x

Angiogenesis is a multi-factorial physiological process deregulated in human diseases characterised by excessive or insufficient blood vessel formation. Emerging evidence highlights a novel role for microRNAs as regulators of angiogenesis. Previous s... Read More about Aberrant expression of miR-133a in endothelial cells inhibits angiogenesis by reducing pro-angiogenic but increasing anti-angiogenic gene expression.

Translational challenges and opportunities in biofilm science: a BRIEF for the future (2022)
Journal Article
Highmore, C. J., Melaugh, G., Morris, R. J., Parker, J., Direito, S. O. L., Romero, M., …Bamford, N. C. (2022). Translational challenges and opportunities in biofilm science: a BRIEF for the future. npj Biofilms and Microbiomes, 8, Article 68. https://doi.org/10.1038/s41522-022-00327-7

Biofilms are increasingly recognised as a critical global issue in a multitude of industries impacting health, food and water security, marine sector, and industrial processes resulting in estimated economic cost of $5 trillion USD annually. A major... Read More about Translational challenges and opportunities in biofilm science: a BRIEF for the future.

Combined docking and machine learning identify key molecular determinants of ligand pharmacological activity on β2 adrenoceptor (2022)
Journal Article
Jiménez-Rosés, M., Morgan, B. A., Jimenez Sigstad, M., Tran, T. D. Z., Srivastava, R., Bunsuz, A., …Veprintsev, D. B. (2022). Combined docking and machine learning identify key molecular determinants of ligand pharmacological activity on β2 adrenoceptor. Pharmacology Research and Perspectives, 10(5), Article e00994. https://doi.org/10.1002/prp2.994

G protein-coupled receptors (GPCRs) are valuable therapeutic targets for many diseases. A central question of GPCR drug discovery is to understand what determines the agonism or antagonism of ligands that bind them. Ligands exert their action via the... Read More about Combined docking and machine learning identify key molecular determinants of ligand pharmacological activity on β2 adrenoceptor.

ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa (2022)
Journal Article
Camara, M., Dubern, J., Romero, M., Mai-Prochnow, A., Messina, M., Trampari, E., …Heeb, S. (2022). ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa. npj Biofilms and Microbiomes, 8, Article 64. https://doi.org/10.1038/s41522-022-00325-9

Pseudomonas aeruginosa uses multiple protein regulators that work in tandem to control the production of a wide range of virulence factors and facilitate rapid adaptation to diverse environmental conditions. In this opportunistic pathogen, ToxR was k... Read More about ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa.

Environmental pharmacology—Dosing the environment: IUPHAR review 36 (2022)
Journal Article
Connolly, C. N., Alexander, S. P., Davies, J. A., & Spedding, M. (2022). Environmental pharmacology—Dosing the environment: IUPHAR review 36. British Journal of Pharmacology, 179(23), 5172-5179. https://doi.org/10.1111/bph.15933

Pesticide action is predominantly measured as a toxicological outcome, with pharmacological impact of sublethal doses on bystander species left largely undocumented. Likewise, chronic exposure, which often results in responses different from acute ad... Read More about Environmental pharmacology—Dosing the environment: IUPHAR review 36.

Natural adaptation and human selection of northeast African sheep genomes (2022)
Journal Article
Ahbara, A. M., Musa, H. H., Robert, C., Abebe, A., Al-Jumaili, A. S., Kebede, A., …Mwacharo, J. M. (2022). Natural adaptation and human selection of northeast African sheep genomes. Genomics, 114(5), Article 110448. https://doi.org/10.1016/j.ygeno.2022.110448

African sheep manifest diverse but distinct physio-anatomical traits, which are the outcomes of natural- and human-driven selection. Here, we generated 34.8 million variants from 150 indigenous northeast African sheep genomes sequenced at an average... Read More about Natural adaptation and human selection of northeast African sheep genomes.