Aberrant expression of miR-133a in endothelial cells inhibits angiogenesis by reducing pro-angiogenic but increasing anti-angiogenic gene expression

Ahmed, Suhail; Kurusamy, Sathishkumar; David, Ezra Leander Santhosh; Khan, Kinza; Kalyanakrishnan, Krithika; Ian-Gobo, Miebaka; Kola, Teja Manidhar; Wilkinson, Robert N.; Kannappan, Vinodh; Wang, Weiguang; Gómez, Manuel J.; Redondo, Juan Miguel; Cotton, James; Armesilla, Angel L.

Abstract

Angiogenesis is a multi-factorial physiological process deregulated in human diseases characterised by excessive or insufficient blood vessel formation. Emerging evidence highlights a novel role for microRNAs as regulators of angiogenesis. Previous studies addressing the effect of miR-133a expression in endothelial cells during blood vessel formation have reported conflicting results. Here, we have assessed the specific effect of mature miR-133a strands in angiogenesis and the expression of endothelial angiogenic genes. Transfection of miR-133a-3p or -5p mimics in primary human endothelial cells significantly inhibited proliferation, migration, and tubular morphogenesis of transfected cells. Screening of gene arrays related to angiogenic processes, and further validation by TaqMan qPCR, revealed that aberrant expression of miR-133a-3p led to a decrease in the expression of genes encoding pro-angiogenic molecules, whilst increasing those with anti-angiogenic functions. Ingenuity Pathway Analysis of a collection of genes differentially expressed in cells harbouring miR-133a-3p, predicted decreased cellular functions related to vasculature branching and cell cycle progression, underlining the inhibitory role of miR-133a-3p in angiogenic cellular processes. Our results suggest that controlled delivery of miR-133a-3p mimics, or antagomirs in diseased endothelial cells, might open new therapeutic interventions to treat patients suffering from cardiovascular pathologies that occur with excessive or insufficient angiogenesis.

Citation

Ahmed, S., Kurusamy, S., David, E. L. S., Khan, K., Kalyanakrishnan, K., Ian-Gobo, M., Kola, T. M., Wilkinson, R. N., Kannappan, V., Wang, W., Gómez, M. J., Redondo, J. M., Cotton, J., & Armesilla, A. L. (2022). Aberrant expression of miR-133a in endothelial cells inhibits angiogenesis by reducing pro-angiogenic but increasing anti-angiogenic gene expression. Scientific Reports, 12(1), Article 14730. https://doi.org/10.1038/s41598-022-19172-x

• Journal Article Type: Article
• Acceptance Date: Aug 25, 2022
• Online Publication Date: Aug 30, 2022
• Publication Date: Aug 30, 2022
• Deposit Date: Nov 10, 2022
• Publicly Available Date: Nov 15, 2022
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 1
• Article Number: 14730
• DOI: https://doi.org/10.1038/s41598-022-19172-x
• Keywords: Humans; MicroRNAs; Genetics metabolism; Morphogenesis; Transfection; Gene Expression; Endothelial Cells metabolism
• Public URL: https://nottingham-repository.worktribe.com/output/11462056
• Publisher URL: https://www.nature.com/articles/s41598-022-19172-x