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Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice

Morgan, Hannah L.; Furse, Samuel; Dias, Irundika H. K.; Shabir, Kiran; Castellanos, Marcos; Khan, Iqbal; May, Sean T.; Holmes, Nadine; Carlile, Matthew; Sang, Fei; Wright, Victoria; Koulman, Albert; Watkins, Adam J.


Samuel Furse

Irundika H. K. Dias

Kiran Shabir

Marcos Castellanos

Iqbal Khan

Nadine Holmes

Matthew Carlile

Fei Sang

Victoria Wright

Albert Koulman


The underlying mechanisms driving paternally-programmed metabolic disease in offspring remain poorly defined. We fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. Using artificial insemination, in combination with vasectomised male mating, we generated offspring using either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Offspring from either LPD sperm or seminal fluid display elevated body weight and tissue dyslipidaemia from just 3 weeks of age. These changes become more pronounced in adulthood, occurring in conjunction with altered hepatic metabolic and inflammatory pathway gene expression. Second generation offspring also display differential tissue lipid abundance, with profiles similar to those of first generation adults. These findings demonstrate that offspring metabolic homeostasis is perturbed in response to a suboptimal paternal diet with the effects still evident within a second generation.


Morgan, H. L., Furse, S., Dias, I. H. K., Shabir, K., Castellanos, M., Khan, I., …Watkins, A. J. (2022). Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice. Communications Biology, 5(1), Article 929.

Journal Article Type Article
Acceptance Date Aug 30, 2022
Online Publication Date Sep 8, 2022
Publication Date Sep 8, 2022
Deposit Date Feb 24, 2023
Publicly Available Date Feb 27, 2023
Journal Communications Biology
Electronic ISSN 2399-3642
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 5
Issue 1
Article Number 929
Keywords General Agricultural and Biological Sciences; General Biochemistry, Genetics and Molecular Biology; Medicine (miscellaneous)
Public URL
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