All Outputs (207)

A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches (2019)
Journal Article
Kumar, P., Ciftci, S., Barthes, J., Knopf-Marques, H., Muller, C. B., Debry, C., …Ghaemmaghami, A. M. (2020). A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches. Journal of Tissue Engineering and Regenerative Medicine, 14(1), 45-57. https://doi.org/10.1002/term.2962

Here we report fabrication of Gelatin based biocomposite films and their application in developing epithelial patches. The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic (ECM mimic) for in v... Read More about A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches.

Inhibition of Piezo1 attenuates demyelination in the central nervous system (2019)
Journal Article
Velasco‐Estevez, M., Gadalla, K. K. E., Liñan‐Barba, N., Cobb, S., Dev, K. K., & Sheridan, G. K. (2020). Inhibition of Piezo1 attenuates demyelination in the central nervous system. Glia, 68(2), 356-375. https://doi.org/10.1002/glia.23722

Piezo1 is a mechanosensitive ion channel that facilitates the translation of extracellular mechanical cues to intracellular molecular signaling cascades through a process termed, mechanotransduction. In the central nervous system (CNS), mechanically... Read More about Inhibition of Piezo1 attenuates demyelination in the central nervous system.

The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis (2019)
Journal Article
Capalbo, L., Bassi, Z. I., Geymonat, M., Todesca, S., Copoiu, L., Enright, A. J., …D’Avino, P. P. (2019). The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nature Communications, 10(1), Article 4513. https://doi.org/10.1038/s41467-019-12507-9

The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cili... Read More about The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.

NanoBiT Complementation to Monitor Agonist-Induced Adenosine A1 Receptor Internalization (2019)
Journal Article
Soave, M., Kellam, B., Woolard, J., Briddon, S. J., & Hill, S. J. (2019). NanoBiT Complementation to Monitor Agonist-Induced Adenosine A1 Receptor Internalization. Slas Discovery, https://doi.org/10.1177/2472555219880475

Receptor internalization in response to prolonged agonist treatment is an important regulator of G protein–coupled receptor (GPCR) function. The adenosine A1 receptor (A1AR) is one of the adenosine receptor family of GPCRs, and evidence for its agoni... Read More about NanoBiT Complementation to Monitor Agonist-Induced Adenosine A1 Receptor Internalization.

Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation (2019)
Journal Article
Dundas, A. A., Sanni, O., Dubern, J., Dimitrakis, G., Hook, A. L., Irvine, D. J., …Alexander, M. R. (2019). Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation. Advanced Materials, 31(49), Article 1903513. https://doi.org/10.1002/adma.201903513

ynthetic materials are an everyday component of modern healthcare yet often fail routinely as a consequence of medical‐device‐centered infections. The incidence rate for catheter‐associated urinary tract infections is between 3% and 7% for each day o... Read More about Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation.

Structure-Kinetic Profiling of Haloperidol Analogues at the Human Dopamine D2 Receptor (2019)
Journal Article
Fyfe, T. J., Kellam, B., Sykes, D. A., Capuano, B., Scammells, P. J., Lane, J. R., …Mistry, S. N. (2019). Structure-Kinetic Profiling of Haloperidol Analogues at the Human Dopamine D2 Receptor. Journal of Medicinal Chemistry, 62(21), 9488-9520. https://doi.org/10.1021/acs.jmedchem.9b00864

Haloperidol is a typical antipsychotic drug (APD) associated with an increased risk of extrapyramidal side-effects (EPS) and hyperprolactinemia relative to atypical APDs such as clozapine. Both drugs are dopamine D2 receptor (D2R) antagonists, with c... Read More about Structure-Kinetic Profiling of Haloperidol Analogues at the Human Dopamine D2 Receptor.

Transporting cells over several days without dry-ice (2019)
Journal Article
Wheatley, S. P., & Wheatley, D. N. (2019). Transporting cells over several days without dry-ice. Journal of Cell Science, 132(21), jcs238139. https://doi.org/10.1242/jcs.238139

This paper describes a simple, hazard-free and inexpensive procedure that allows researchers to send cultured cells across the globe at ambient temperatures. The method enables transit of up to 2?weeks without compromising cell recovery. Its use will... Read More about Transporting cells over several days without dry-ice.

Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in (2019)
Journal Article
Khan, A. O., White, C. W., Pike, J. A., Yule, J., Slater, A., Hill, S. J., …Morgan, N. V. (2019). Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in. Scientific Reports, 9(1), Article 14219. https://doi.org/10.1038/s41598-019-50733-9

© 2019, The Author(s). The use of CRISPR-Cas9 genome editing to introduce endogenously expressed tags has the potential to address a number of the classical limitations of single molecule localisation microscopy. In this work we present the first sys... Read More about Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in.

Identification of key residues that regulate the interaction of kinesins with microtubule ends (2019)
Journal Article
Belsham, H. R., & Friel, C. T. (2019). Identification of key residues that regulate the interaction of kinesins with microtubule ends. Cytoskeleton, 76(7-8), 440-446. https://doi.org/10.1002/cm.21568

Kinesins are molecular motors that use energy derived from ATP turnover to walk along microtubules, or when at the microtubule end, regulate growth or shrinkage. All kinesins that regulate microtubule dynamics have long residence times at microtubule... Read More about Identification of key residues that regulate the interaction of kinesins with microtubule ends.

Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission (2019)
Journal Article
Zeeshan, M., Shilliday, F., Liu, T., Abel, S., Mourier, T., Ferguson, D. J. P., …Tewari, R. (2019). Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission. PLoS Pathogens, 15(10), Article e1008048. https://doi.org/10.1371/journal.ppat.1008048

Kinesin-8 proteins are microtubule motors that are often involved in regulation of mitotic spindle length and chromosome alignment. They move towards the plus ends of spindle microtubules and regulate the dynamics of these ends due, at least in some... Read More about Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission.

Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men (2019)
Journal Article
Gharahdaghi, N., Rudrappa, S., Brook, M. S., Idris, I., Crossland, H., Hamrock, C., …Atherton, P. J. (2019). Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men. Journal of Cachexia, Sarcopenia and Muscle, 10(6), 1276-1294. https://doi.org/10.1002/jcsm.12472

Background: The andropause is associated with declines in serum testosterone (T), loss of muscle mass (sarcopenia) and frailty. Two major interventions purported to offset sarcopenia are anabolic steroid therapies and resistance exercise training (RE... Read More about Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men.

The impact of reduced red and processed meat consumption on cardiovascular risk factors; an intervention trial in healthy volunteers (2019)
Journal Article
Simpson, E. J., Clark, M., Razak, A. A., & Salter, A. (2019). The impact of reduced red and processed meat consumption on cardiovascular risk factors; an intervention trial in healthy volunteers. Food and Function, 2019(10), 6690-6698. https://doi.org/10.1039/C9FO00758J

Meat represents an important part of the diet for many adults, supplying essential amino acids and micronutrients. However, high red and processed meat (RPM) intake is implicated in the development of cardiovascular disease and dyslipidemia. This stu... Read More about The impact of reduced red and processed meat consumption on cardiovascular risk factors; an intervention trial in healthy volunteers.

Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale (2019)
Journal Article
Goertz, S., de Menezes, A. B., Birtles, R. J., Fenn, J., Lowe, A. E., MacColl, A. D., …Taylor, C. H. (2019). Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale. PLoS ONE, 14(9), Article e0222501. https://doi.org/10.1371/journal.pone.0222501

The composition of the mammalian gut microbiota can be influenced by a multitude of environmental variables such as diet and infections. Studies investigating the effect of these variables on gut microbiota composition often sample across multiple se... Read More about Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale.

Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish (2019)
Journal Article
Chhabria, K., Vouros, A., Gray, C., MacDonald, R. B., Jiang, Z., Wilkinson, R. N., …Chico, T. J. (2019). Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish. Disease Models and Mechanisms, 12(9), https://doi.org/10.1242/dmm.039867

Diabetes is associated with dysfunction of the neurovascular unit, although the mechanisms of this are incompletely understood and currently no treatment exists to prevent these negative effects. We previously found that the nitric oxide (NO) donor s... Read More about Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish.

Sex-specific hippocampal metabolic signatures at the onset of systemic inflammation with lipopolysaccharide in the APPswe/PS1dE9 mouse model of Alzheimer's disease (2019)
Journal Article
Agostini, A., Yuchun, D., Li, B., Kendall, D. A., & Pardon, M. (2020). Sex-specific hippocampal metabolic signatures at the onset of systemic inflammation with lipopolysaccharide in the APPswe/PS1dE9 mouse model of Alzheimer's disease. Brain, Behavior, and Immunity, 83, 87-111. https://doi.org/10.1016/j.bbi.2019.09.019

Systemic inflammation enhances the risk and progression of Alzheimer's disease (AD). Lipopolysaccharide (LPS), a potent pro-inflammatory endotoxin produced by the gut, is found in excess levels in AD where it associates with neurological hallmarks of... Read More about Sex-specific hippocampal metabolic signatures at the onset of systemic inflammation with lipopolysaccharide in the APPswe/PS1dE9 mouse model of Alzheimer's disease.

Simultaneous Tracking of Pseudomonas aeruginosa motility in liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators (2019)
Journal Article
Hook, A. L., Flewellen, J. L., Dubern, J., Carabelli, A., Zald, I. M., Berry, R. M., …Alexander, M. R. (2019). Simultaneous Tracking of Pseudomonas aeruginosa motility in liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators. mSystems, 4(5), Article e00390-19. https://doi.org/10.1128/mSystems.00390-19

Bacteria sense chemicals, surfaces, and other cells and move toward some and away from others. Studying how single bacterial cells in a population move requires sophisticated tracking and imaging techniques. We have established quantitative methodolo... Read More about Simultaneous Tracking of Pseudomonas aeruginosa motility in liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators.

Modulators of CXCR4 and CXCR7/ACKR3 Function (2019)
Journal Article
Adlere, I., Caspar, B., Arimont, M., Dekkers, S., Visser, K., Stuijt, J., …Leurs, R. (2019). Modulators of CXCR4 and CXCR7/ACKR3 Function. Molecular Pharmacology, 96(6), 737-752. https://doi.org/10.1124/mol.119.117663

Copyright © 2019 by The Author(s). The two G protein-coupled receptors (GPCRs) C-X-C chemokine receptor type 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3) are part of the class A chemokine GPCR family and represent important drug targets for hu... Read More about Modulators of CXCR4 and CXCR7/ACKR3 Function.

Ligand-dependent spatiotemporal signaling profiles of the mu-opioid receptor are controlled by distinct protein-interaction networks (2019)
Journal Article
Civciristov, S., Huang, C., Liu, B., Marquez, E. A., Gondin, A. B., Schittenhelm, R. B., …Halls, M. L. (2019). Ligand-dependent spatiotemporal signaling profiles of the mu-opioid receptor are controlled by distinct protein-interaction networks. Journal of Biological Chemistry, 294(44), 16198-16213. https://doi.org/10.1074/jbc.ra119.008685

Ligand-dependent differences in the regulation and internalization of the mu-opioid receptor (MOR) have been linked to the severity of adverse effects that limit opiate use in pain management. MOR activation by morphine or [D-Ala2,N-MePhe4,Gly-ol]-en... Read More about Ligand-dependent spatiotemporal signaling profiles of the mu-opioid receptor are controlled by distinct protein-interaction networks.

Cognition in stroke rehabilitation and recovery research: Consensus-based core recommendations from the second Stroke Recovery and Rehabilitation Roundtable (2019)
Journal Article
McDonald, M. W., Black, S. E., Copland, D. A., Corbett, D., Dijkhuizen, R. M., Farr, T. D., …O’Sullivan, M. J. (2019). Cognition in stroke rehabilitation and recovery research: Consensus-based core recommendations from the second Stroke Recovery and Rehabilitation Roundtable. International Journal of Stroke, 14(8), 774-782. https://doi.org/10.1177/1747493019873600

© 2019 World Stroke Organization. Cognitive impairment is an important target for rehabilitation as it is common following stroke, is associated with reduced quality of life and interferes with motor and other types of recovery interventions. Cogniti... Read More about Cognition in stroke rehabilitation and recovery research: Consensus-based core recommendations from the second Stroke Recovery and Rehabilitation Roundtable.

A complete genome sequence of Cupriavidus necator H16 (DSM 428) (2019)
Journal Article
Little, G. T., Ehsaan, M., Arenas-López, C., Jawed, K., Winzer, K., Kovacs, K., & Minton, N. P. (2019). A complete genome sequence of Cupriavidus necator H16 (DSM 428). Microbiology Resource Announcements, 8(37), 1-2. https://doi.org/10.1128/MRA.00814-19

The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation of this strain reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA genes, and 15 rRNA genes.