The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis

Capalbo, Luisa; Bassi, Zuni I.; Geymonat, Marco; Todesca, Sofia; Copoiu, Liviu; Enright, Anton J.; Callaini, Giuliano; Riparbelli, Maria Giovanna; Yu, Lu; Choudhary, Jyoti S.; Ferrero, Enrico; Wheatley, Sally; Douglas, Max E.; Mishima, Masanori; D�Avino, Pier Paolo

Abstract

The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many previously unknown interactions and provides an extremely valuable resource for dissecting the multiple roles of the midbody. Initial analysis of this interactome revealed that PP1β-MYPT1 phosphatase regulates microtubule dynamics in late cytokinesis and de-phosphorylates the kinesin component MKLP1/KIF23 of the centralspindlin complex. This de-phosphorylation antagonizes Aurora B kinase to modify the functions and interactions of centralspindlin in late cytokinesis. Our findings expand the repertoire of PP1 functions during mitosis and indicate that spatiotemporal changes in the distribution of kinases and counteracting phosphatases finely tune the activity of cytokinesis proteins.

Citation

Capalbo, L., Bassi, Z. I., Geymonat, M., Todesca, S., Copoiu, L., Enright, A. J., …D’Avino, P. P. (2019). The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nature Communications, 10(1), Article 4513. https://doi.org/10.1038/s41467-019-12507-9

• Journal Article Type: Article
• Acceptance Date: Sep 13, 2019
• Online Publication Date: Oct 4, 2019
• Publication Date: 2019-12
• Deposit Date: Apr 27, 2020
• Publicly Available Date: Apr 27, 2020
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 1
• Article Number: 4513
• DOI: https://doi.org/10.1038/s41467-019-12507-9
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Physics and Astronomy; General Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/3017619
• Publisher URL: https://www.nature.com/articles/s41467-019-12507-9
• Additional Information: Received: 20 February 2019; Accepted: 13 September 2019; First Online: 4 October 2019; : The authors declare no competing interests.