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Mitotic activity of survivin is regulated by acetylation at K129

Aljaberi, Aysha M.; Webster, Jamie R.M.; Wheatley, Sally P.

Authors

Aysha M. Aljaberi

Jamie R.M. Webster

Sally P. Wheatley



Abstract

Survivin is a cancer-associated protein regulated by multiple factors, including acetylation at K129 within its C-terminal alpha-helical tail. Acetylation of survivin is being pursued as a potential prognostic marker in breast cancer. This modification at K129 may cause nuclear accumulation of survivin in interphase cells; however, whether this affects its essential role during mitosis has not been addressed. We posited whether mimicking acetylation of survivin at K129 alters its activity during mitosis. Fluorescence microscopy and time-lapse imaging showed that, mutating this site to an alanine to act as a constitutive acetyl mimetic, K129A, causes defects in chromosome segregation and cytokinesis. As a non-acetylatable version, K129R, also has difficulty during mitotic exit, we conclude that cyclical acetylation and deacetylation is required for fully functional survivin during mitosis.

Journal Article Type Article
Publication Date Jun 1, 2015
Journal Cell Cycle
Print ISSN 1538-4101
Electronic ISSN 1551-4005
Publisher Taylor & Francis Open
Peer Reviewed Peer Reviewed
Volume 14
Issue 11
APA6 Citation Aljaberi, A. M., Webster, J. R., & Wheatley, S. P. (2015). Mitotic activity of survivin is regulated by acetylation at K129. Cell Cycle, 14(11), https://doi.org/10.1080/15384101.2015.1033597
DOI https://doi.org/10.1080/15384101.2015.1033597
Keywords apoptosis; acetylation; cancer; mitosis; survivin
Publisher URL http://www.tandfonline.com/doi/full/10.1080/15384101.2015.1033597
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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