@article { , title = {Mitotic activity of survivin is regulated by acetylation at K129}, abstract = {Survivin is a cancer-associated protein regulated by multiple factors, including acetylation at K129 within its C-terminal alpha-helical tail. Acetylation of survivin is being pursued as a potential prognostic marker in breast cancer. This modification at K129 may cause nuclear accumulation of survivin in interphase cells; however, whether this affects its essential role during mitosis has not been addressed. We posited whether mimicking acetylation of survivin at K129 alters its activity during mitosis. Fluorescence microscopy and time-lapse imaging showed that, mutating this site to an alanine to act as a constitutive acetyl mimetic, K129A, causes defects in chromosome segregation and cytokinesis. As a non-acetylatable version, K129R, also has difficulty during mitotic exit, we conclude that cyclical acetylation and deacetylation is required for fully functional survivin during mitosis.}, doi = {10.1080/15384101.2015.1033597}, eissn = {1551-4005}, issn = {1538-4101}, issue = {11}, journal = {Cell Cycle}, publicationstatus = {Published}, publisher = {Taylor \& Francis Open}, url = {https://nottingham-repository.worktribe.com/output/750327}, volume = {14}, keyword = {apoptosis, acetylation, cancer, mitosis, survivin}, year = {2015}, author = {Aljaberi, Aysha M. and Webster, Jamie R.M. and Wheatley, Sally P.} }