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Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission

Zeeshan, Mohammad; Shilliday, Fiona; Liu, Tianyang; Abel, Steven; Mourier, Tobias; Ferguson, David J P; Rea, Edward; Stanway, Rebecca R; Roques, Magali; Williams, Desiree; Daniel, Emilie; Brady, Declan; Roberts, Anthony J; Holder, Anthony A; Pain, Arnab; Le, Karine G; Le Roch, Roch G; Moores, Carolyn A; Tewari, Rita

Authors

Mohammad Zeeshan

Fiona Shilliday

Tianyang Liu

Steven Abel

Tobias Mourier

David J P Ferguson

Edward Rea

Rebecca R Stanway

Magali Roques

Desiree Williams

Emilie Daniel

Declan Brady

Anthony J Roberts

Anthony A Holder

Arnab Pain

Karine G Le

Roch G Le Roch

Carolyn A Moores

Rita Tewari



Abstract

Kinesin-8 proteins are microtubule motors that are often involved in regulation of mitotic spindle length and chromosome alignment. They move towards the plus ends of spindle microtubules and regulate the dynamics of these ends due, at least in some species, to their microtubule depolymerization activity. Plasmodium spp. exhibit an atypical endomitotic cell division in which chromosome condensation and spindle dynamics in the different proliferative stages are not well understood. Genome-wide shared orthology analysis of Plasmodium spp. revealed the presence of two kinesin-8 motor proteins, kinesin-8X and kinesin-8B. Here we studied the biochemical properties of kinesin-8X and its role in parasite proliferation. In vitro, kinesin-8X has motility and depolymerization activities like other kinesin-8 motors. To understand the role of Plasmodium kinesin-8X in cell division, we used fluorescence-tagging and live cell Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation

Journal Article Type Article
Publication Date Oct 10, 2019
Journal PLOS Pathogens
Print ISSN 1553-7366
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 15
Issue 10
Article Number e1008048
APA6 Citation Zeeshan, M., Shilliday, F., Liu, T., Abel, S., Mourier, T., Ferguson, D. J. P., …Tewari, R. (2019). Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission. PLoS Pathogens, 15(10), https://doi.org/10.1371/journal.ppat.1008048
DOI https://doi.org/10.1371/journal.ppat.1008048
Keywords Immunology; Genetics; Molecular Biology; Microbiology; Parasitology; Virology

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