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Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission

Le Roch, Karine G.; Holder, Anthony A.; Bottrill, Andrew R.; Stanway, Rebecca R.; Wall, Richard J.; Tewari, Rita; Pandey, Rajan; Abel, Steven; Boucher, Matthew; Zeeshan, Mohammad; Wheatley, Sally; Batugedara, Gayani; Hollin, Thomas; Daniel, Emilie; Rea, Edward; Freville, Aline; Lu, Xueqing Maggie; Gupta, Dinesh; Brady, Declan

Authors

Karine G. Le Roch

Anthony A. Holder

Andrew R. Bottrill

Rebecca R. Stanway

Richard J. Wall

RITA TEWARI RITA.TEWARI@NOTTINGHAM.AC.UK
Professor of Parasite Cell Biology

Rajan Pandey

Steven Abel

Matthew Boucher

Gayani Batugedara

Thomas Hollin

Emilie Daniel

Edward Rea

Aline Freville

Xueqing Maggie Lu

Dinesh Gupta

Declan Brady



Abstract

Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin, during endomitosis in schizogony and endoreduplication in male gametogenesis. During early schizogony, SMC2/SMC4 localize to a distinct focus, identified as the centromeres by NDC80 fluorescence and chromatin immunoprecipitation sequencing (ChIP-seq) analyses, but do not form condensin I or II complexes. In mature schizonts and during male gametogenesis, there is a diffuse SMC2/SMC4 distribution on chromosomes and in the nucleus, and both condensin I and condensin II complexes form at these stages. Knockdown of smc2 and smc4 gene expression reveals essential roles in parasite proliferation and transmission. The condensin core subunits (SMC2/SMC4) form different complexes and may have distinct functions at various stages of the parasite life cycle.

Journal Article Type Article
Publication Date Feb 11, 2020
Journal Cell Reports
Print ISSN 2211-1247
Electronic ISSN 2211-1247
Publisher Elsevier (Cell Press)
Peer Reviewed Peer Reviewed
Volume 30
Issue 6
Pages 1883-1897.e6
APA6 Citation Le Roch, K. G., Holder, A. A., Bottrill, A. R., Stanway, R. R., Wall, R. J., Tewari, R., …Brady, D. (2020). Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission. Cell Reports, 30(6), 1883-1897.e6. https://doi.org/10.1016/j.celrep.2020.01.033
DOI https://doi.org/10.1016/j.celrep.2020.01.033
Publisher URL https://www.sciencedirect.com/science/article/pii/S2211124720300486
Additional Information This article is maintained by: Elsevier; Article Title: Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission; Journal Title: Cell Reports; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.celrep.2020.01.033; Content Type: article; Copyright: © 2020 The Authors.

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