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A design of experiments (DoE) approach to identify the influencing parameters that determine poly-D,L-lactic acid (PDLLA) electrospun scaffold morphologies (2017)
Journal Article
Ruiter, F. A. A., Alexander, C., Rose, F. R., & Segal, J. (2017). A design of experiments (DoE) approach to identify the influencing parameters that determine poly-D,L-lactic acid (PDLLA) electrospun scaffold morphologies. Biomedical Materials, 12(5), Article 055009. https://doi.org/10.1088/1748-605X/aa7b54

Electrospun fibrous materials have increasing applications in regenerative medicine due to the similarity of fibre constructs to the morphology of certain extracellular matrices. Although experimentally the electrospinning method is relatively simple... Read More about A design of experiments (DoE) approach to identify the influencing parameters that determine poly-D,L-lactic acid (PDLLA) electrospun scaffold morphologies.

Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania mexicana (2017)
Journal Article
Mwenechanya, R., Kovarova, J., Dickens, N. J., Mudaliar, M., Herzyk, P., Vicent, I. M., …Barrett, M. P. (2017). Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania mexicana. PLoS Neglected Tropical Diseases, 11(6), Article e0005649. https://doi.org/10.1371/journal.pntd.0005649

Amphotericin B has emerged as the therapy of choice for use against the leishmaniases. Administration of the drug in its liposomal formulation as a single injection is being promoted in a campaign to bring the leishmaniases under control. Understandi... Read More about Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania mexicana.

Image based machine learning for identification of macrophage subsets (2017)
Journal Article
Rostam, H., Reynolds, P. M., Alexander, M. R., Gadegaard, N., & Ghaemmaghami, A. M. (2017). Image based machine learning for identification of macrophage subsets. Scientific Reports, 7(1), Article 3521. https://doi.org/10.1038/s41598-017-03780-z

Macrophages play a crucial rule in orchestrating immune responses against pathogens and foreign materials. Macrophages have remarkable plasticity in response to environmental cues and are able to acquire a spectrum of activation status, best exemplif... Read More about Image based machine learning for identification of macrophage subsets.

In vitro antitumor effects of AHR ligands aminoflavone (AFP 464) and benzothiazole (5F 203) in human renal carcinoma cells (2017)
Journal Article
Luzzani, G. A., Callero, M. A., Kuruppu, A. I., Trapani, V., Flumian, C., Todaro, L., …Loaiza Perez, A. I. (in press). In vitro antitumor effects of AHR ligands aminoflavone (AFP 464) and benzothiazole (5F 203) in human renal carcinoma cells. Journal of Cellular Biochemistry, https://doi.org/10.1002/jcb.26114

We investigated activity and mechanism of action of two AhR ligand antitumor agents, AFP 464 and 5F 203 on human renal cancer cells, specifically examining their effects on cell cycle progression, apoptosis, and migration. TK-10, SN12C, Caki-1, and A... Read More about In vitro antitumor effects of AHR ligands aminoflavone (AFP 464) and benzothiazole (5F 203) in human renal carcinoma cells.

3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review (2017)
Journal Article
Prina, E., Mistry, P., Sidney, L. E., Yang, J., Wildman, R. D., Bertolin, M., …Rose, F. R. A. (2017). 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review. Stem Cell Reviews and Reports, 13(3), 430-441. https://doi.org/10.1007/s12015-017-9740-6

In recent years, there has been increased research interest in generating corneal substitutes, either for use in the clinic or as in vitro corneal models. The advancement of 3D microfabrication technologies has allowed the reconstruction of the nativ... Read More about 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review.

Novel hybrids of natural ?-elemene bearing isopropanolamine moieties: synthesis, enhanced anticancer profile, and improved aqueous solubility (2017)
Journal Article
Chen, J., Wang, T., Xu, S., Aijun, L., Yao, H., Xie, W., …Xu, J. (2017). Novel hybrids of natural β-elemene bearing isopropanolamine moieties: synthesis, enhanced anticancer profile, and improved aqueous solubility. Fitoterapia, 120, https://doi.org/10.1016/j.fitote.2017.05.002

A series of novel ?-elemene isopropanolamine derivatives were synthesized and evaluated for their antitumor activity. The results indicated that all of the compounds showed stronger antiproliferative activities than ?-elemene as well as improved aque... Read More about Novel hybrids of natural ?-elemene bearing isopropanolamine moieties: synthesis, enhanced anticancer profile, and improved aqueous solubility.

A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study (2017)
Journal Article
Abdelrazig, S. M., Ortori, C. A., Davey, G., Deressa, W., Mulleta, D., Barrett, D. A., …Fogarty, A. W. (2017). A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study. Malaria Journal, 16, Article 229. https://doi.org/10.1186/s12936-017-1875-z

BACKGROUND: Currently available diagnostic techniques of Plasmodium falciparum infection are not optimal for non-invasive, population-based screening for malaria. It was hypothesized that a mass spectrometry-based metabolomics approach could identify... Read More about A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study.

Development of cordycepin formulations for preclinical and clinical studies (2017)
Journal Article
Lee, J. B., Adrower, C., Qin, C., Fischer, P. M., de Moor, C. H., & Gershkovich, P. (2017). Development of cordycepin formulations for preclinical and clinical studies. AAPS PharmSciTech, 18(8), 3219-3226. https://doi.org/10.1208/s12249-017-0795-0

There is extensive literature on in vivo studies with cordycepin but these studies were generally conducted without validation of the various formulations, especially in terms of the solubility of cordycepin in the dosing vehicles used. Cordycepin is... Read More about Development of cordycepin formulations for preclinical and clinical studies.

A methodology for elucidating regulatory mechanisms leading to changes in lipid profiles (2017)
Journal Article
Casbas Pinto, F., Ravipati, S., Barrett, D. A., & Hodgman, C. (2017). A methodology for elucidating regulatory mechanisms leading to changes in lipid profiles. Metabolomics, 13(7), Article 81. https://doi.org/10.1007/s11306-017-1214-y

Introduction It is difficult to elucidate the metabolic and regulatory factors causing lipidome perturbations. Objectives This work simplifies this process. Methods A method has been developed to query an online holistic lipid metabolic netwo... Read More about A methodology for elucidating regulatory mechanisms leading to changes in lipid profiles.

On the suitability of high vacuum electrospray deposition for the fabrication of molecular electronic devices (2017)
Journal Article
Temperton, R. H., O'Shea, J. N., & Scurr, D. J. (2017). On the suitability of high vacuum electrospray deposition for the fabrication of molecular electronic devices. Chemical Physics Letters, 682, 15-19. https://doi.org/10.1016/j.cplett.2017.05.068

© 2017 We present a series of three studies investigating the potential application of high vacuum electrospray deposition to construct molecular electronic devices. Through the use of time of flight secondary ion mass spectrometry we explore the use... Read More about On the suitability of high vacuum electrospray deposition for the fabrication of molecular electronic devices.

Endotoxin testing of a wound debridement device containing medicinal Lucilia sericata larvae (2017)
Journal Article
Pickles, S., & Pritchard, D. I. (2017). Endotoxin testing of a wound debridement device containing medicinal Lucilia sericata larvae. Wound Repair and Regeneration, 25(3), https://doi.org/10.1111/wrr.12539

Alimentary products of medicinal Lucilia sericata larvae are studied to determine their mechanisms of action, particularly in the contexts of wound debridement and disinfection. Furthermore, the larvae can be applied to patients in contained devices,... Read More about Endotoxin testing of a wound debridement device containing medicinal Lucilia sericata larvae.

Enhanced expression of Organic Cation Transporters in bronchial epithelial cell layers following insults associated with asthma – impact on salbutamol transport (2017)
Journal Article
Mukherjee, M., Cingolani, E., Pritchard, D., & Bosquillon, C. (2017). Enhanced expression of Organic Cation Transporters in bronchial epithelial cell layers following insults associated with asthma – impact on salbutamol transport. European Journal of Pharmaceutical Sciences, 106, https://doi.org/10.1016/j.ejps.2017.05.052

Increasing evidence suggests Organic Cation Transporters (OCT) might facilitate the absorption of inhaled bronchodilators, including salbutamol, across the lung epithelium. This is essentially scarred and inflamed in asthma. Accordingly, the impact o... Read More about Enhanced expression of Organic Cation Transporters in bronchial epithelial cell layers following insults associated with asthma – impact on salbutamol transport.

Unveiling a novel transient druggable pocket in BACE-1 through molecular simulations: conformational analysis and binding mode of multisite inhibitors (2017)
Journal Article
Di Pietro, O., Juarez-Jimenez, J., Munoz-Torrero, D., Laughton, C. A., & Luque, F. J. (2017). Unveiling a novel transient druggable pocket in BACE-1 through molecular simulations: conformational analysis and binding mode of multisite inhibitors. PLoS ONE, 12(5), Article e0177683. https://doi.org/10.1371/journal.pone.0177683

The critical role of BACE-1 in the formation of neurotoxic ß-amyloid peptides in the brain makes it an attractive target for an efficacious treatment of Alzheimer’s disease. However, the development of clinically useful BACE-1 inhibitors has proven t... Read More about Unveiling a novel transient druggable pocket in BACE-1 through molecular simulations: conformational analysis and binding mode of multisite inhibitors.

Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology (2017)
Journal Article
Taresco, V., Louzao, I., Scurr, D. J., Turpin, E. R., Laughton, C. A., Alexander, C., …Garnett, M. (in press). Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology. Molecular Pharmaceutics, 14(6), https://doi.org/10.1021/acs.molpharmaceut.7b00182

A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable com... Read More about Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology.

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment (2017)
Journal Article
Xue, X., Thiagarajan, L., Braim, S., Saunders, B. R., Shakesheff, K. M., & Alexander, C. (2017). Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment. Journal of Materials Chemistry B, 5(25), https://doi.org/10.1039/C7TB00052A

We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment. Thermoresponsive poly(N-acryloyl glycinamide)-stat-poly(N-phenylac... Read More about Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment.

Development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating (2017)
Journal Article
Tyler, B. J., Hook, A. L., Pelster, A., Williams, P., Alexander, M. R., & Arlinghaus, H. F. (2017). Development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating. Biointerphases, 12(2), Article 02C412. https://doi.org/10.1116/1.4984011

Catheter associated urinary tract infections (CA-UTIs) are the most common health related infections world wide, contributing significantly to patient morbidity and mortality and increased health care costs. To reduce the incidence of these infection... Read More about Development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating.

High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity (2017)
Book Chapter
Ivanov, D. P., Grabowska, A. M., & Garnett, M. C. (2018). High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity. In D. F. Gilbert, & O. Friedrich (Eds.), Cell Viability Assays (43-59). Humana Press (Springer). https://doi.org/10.1007/978-1-4939-6960-9_4

© 2017, Springer Science+Business Media LLC. Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platfo... Read More about High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity.