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Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology

Taresco, Vincenzo; Louzao, Iria; Scurr, David J.; Turpin, Eleanor R.; Laughton, Charles A.; Alexander, Cameron; Burley, Jonathan C.; Garnett, Martin

Authors

Vincenzo Taresco

Iria Louzao

David J. Scurr David.Scurr@nottingham.ac.uk

Eleanor R. Turpin

Charles A. Laughton charles.laughton@nottingham.ac.uk

Cameron Alexander cameron.alexander@notingham.ac.uk

Jonathan C. Burley jonathan.burley@nottingham.ac.uk

Martin Garnett martin.garnett@nottingham.ac.uk



Abstract

A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable composition were produced in an easily-addressable micro-array format. The amount of material printed for each dried spot ranged from 25 ng to 650 ng. These arrays were used to assess the stability of drug:polymer dispersions with respect to recrystallization, using polarized light microscopy. One array with a panel of 6 drugs formulated at different ratios with Poly (vinylpyrrolidone-vinyl acetate) copolymer (PVPVA) was developed to estimate a possible bulk (gram-scale) approximation threshold from the final printed nano amount of formulation. Another array was printed at a fixed final amount of material to establish a literature comparison of one drug formulated with different commercial polymers for validation. This new approach may offer significant efficiency in pharmaceutical formulation screening, with each experiment in the nano-micro-array format requiring from 3 up to 6 orders of magnitude lower amounts of sample than conventional screening methods.

Journal Article Type Article
Journal Molecular Pharmaceutics
Print ISSN 1543-8384
Electronic ISSN 1543-8384
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 14
Issue 6
APA6 Citation Taresco, V., Louzao, I., Scurr, D. J., Turpin, E. R., Laughton, C. A., Alexander, C., …Garnett, M. (in press). Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology. Molecular Pharmaceutics, 14(6), doi:10.1021/acs.molpharmaceut.7b00182
DOI https://doi.org/10.1021/acs.molpharmaceut.7b00182
Keywords High throughput array, Ink-jet printing, Amorphous dispersion, Recrystallisation, Polarizing microscopy
Publisher URL http://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00182
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Medicinal Chemistry Letters copyright © 2017 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi...s.molpharmaceut.7b00182

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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