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Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology

Taresco, Vincenzo; Louzao, Iria; Scurr, David J.; Turpin, Eleanor R.; Laughton, Charles A.; Alexander, Cameron; Burley, Jonathan C.; Garnett, Martin

Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology Thumbnail


Authors

Iria Louzao

DAVID SCURR DAVID.SCURR@NOTTINGHAM.AC.UK
Principal Research Fellow

Eleanor R. Turpin

CHARLES LAUGHTON CHARLES.LAUGHTON@NOTTINGHAM.AC.UK
Professor of Computational Pharmaceutical Science

Martin Garnett



Abstract

A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable composition were produced in an easily-addressable micro-array format. The amount of material printed for each dried spot ranged from 25 ng to 650 ng. These arrays were used to assess the stability of drug:polymer dispersions with respect to recrystallization, using polarized light microscopy. One array with a panel of 6 drugs formulated at different ratios with Poly (vinylpyrrolidone-vinyl acetate) copolymer (PVPVA) was developed to estimate a possible bulk (gram-scale) approximation threshold from the final printed nano amount of formulation. Another array was printed at a fixed final amount of material to establish a literature comparison of one drug formulated with different commercial polymers for validation. This new approach may offer significant efficiency in pharmaceutical formulation screening, with each experiment in the nano-micro-array format requiring from 3 up to 6 orders of magnitude lower amounts of sample than conventional screening methods.

Citation

Taresco, V., Louzao, I., Scurr, D. J., Turpin, E. R., Laughton, C. A., Alexander, C., …Garnett, M. (in press). Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology. Molecular Pharmaceutics, 14(6), https://doi.org/10.1021/acs.molpharmaceut.7b00182

Journal Article Type Article
Acceptance Date May 14, 2017
Online Publication Date May 14, 2017
Deposit Date May 17, 2017
Publicly Available Date Mar 29, 2024
Journal Molecular Pharmaceutics
Print ISSN 1543-8384
Electronic ISSN 1543-8384
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 14
Issue 6
DOI https://doi.org/10.1021/acs.molpharmaceut.7b00182
Keywords High throughput array, Ink-jet printing, Amorphous dispersion, Recrystallisation, Polarizing microscopy
Public URL https://nottingham-repository.worktribe.com/output/860398
Publisher URL http://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00182
Additional Information This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Medicinal Chemistry Letters copyright © 2017 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00182