All Outputs (45)

Protease sensing with nanoparticle based platforms (2010)
Journal Article

Nanoparticulate systems in various unique configurations are highly effective at detecting protease activity both in vivo and in vitro. In this article, we have summarised the conventional modern methods for monitoring protease activity, and critical... Read More about Protease sensing with nanoparticle based platforms.

Regulation of neurotoxin production and sporulation by a putative agrBD signaling system in proteolytic Clostridium botulinum (2010)
Journal Article

A significant number of genome sequences of Clostridium botulinum and related species have now been determined. In silico analysis of these data revealed the presence of two distinct agr loci (agr-1 and agr-2) in all group I strains, each encoding pu... Read More about Regulation of neurotoxin production and sporulation by a putative agrBD signaling system in proteolytic Clostridium botulinum.

5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: A robust method to screen MAPK pathway allosteric inhibitors (2010)
Journal Article

Fluorescence polarization using Nα-(fluorescein-5- carbonyl)-Nε-(N-[2-fluoro-4-iodophenyl]-3,4- difluoroanthraniloyl)lysyl amide is capable of rapidly identifying inhibitor ligands that bind to an allosteric site in MEK1. © The Royal Society of Chemi... Read More about 5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: A robust method to screen MAPK pathway allosteric inhibitors.

A facile method to clickable sensing polymeric nanoparticles (2009)
Journal Article

Clickable biocompatible nanoparticles were prepared in a one-pot process by microemulsion polymerization using acrylamide, N,N′-methylene bisacrylamide and either N-(11-azido-3,6,9-trioxaundecanyl)acrylamide or N-propargylacrylamide, which were then... Read More about A facile method to clickable sensing polymeric nanoparticles.

Facile synthesis of responsive nanoparticles with reversible, tunable and rapid thermal transitions from biocompatible constituents (2009)
Journal Article

Responsive polymeric nanoparticles composed of hybrid block co-polymers were prepared from biocompatible components that displayed rapid, tunable and multiply reversible transitions in response to change of temperature. © 2009 The Royal Society of Ch... Read More about Facile synthesis of responsive nanoparticles with reversible, tunable and rapid thermal transitions from biocompatible constituents.

Protease responsive nanoprobes with tethered fluorogenic peptidyl 3-arylcoumarin substrates (2008)
Journal Article

Protease responsive nanosensors were obtained by the attachment of unique green fluorescent bifunctional 3-arylcoumarin-derived fluorogenic substrates to poly(acrylamide-co-N-(3-aminopropyl)methacrylamide) nanoparticles, in which proteolysis results... Read More about Protease responsive nanoprobes with tethered fluorogenic peptidyl 3-arylcoumarin substrates.

A macroporous polymer-supported cyclic anhydride for efficient sequestration of amines (2008)
Journal Article

The efficient removal of primary and secondary amines from organic solutions using a macroporous polymer-supported anhydride is described. The sequestering of primary amines by the anhydride via polymer-bound amide formation is completed within 2-4 h... Read More about A macroporous polymer-supported cyclic anhydride for efficient sequestration of amines.

Differential Recognition of Staphylococcus aureus Quorum-Sensing Signals Depends on Both Extracellular Loops 1 and 2 of the Transmembrane Sensor AgrC (2008)
Journal Article

Virulence in Staphylococcus aureus is regulated via agr-dependent quorum sensing in which an autoinducing peptide (AIP) activates AgrC, a histidine protein kinase. AIPs are usually thiolactones containing seven to nine amino acid residues in which th... Read More about Differential Recognition of Staphylococcus aureus Quorum-Sensing Signals Depends on Both Extracellular Loops 1 and 2 of the Transmembrane Sensor AgrC.

Targeting of polyamidoamine-DNA nanoparticles using the Staudinger ligation: Attachment of an RGD motif either before or after complexation (2008)
Journal Article

Two new methods for the modular synthesis of targeted gene delivery systems are reported. The PEGylated polyamidoamine DMEDA-PEG-DMEDA-(MBA-DMEDA)n+1-PEG-DMEDA 3 was sequentially modified to contain an integrin-binding peptide ligand via the Stauding... Read More about Targeting of polyamidoamine-DNA nanoparticles using the Staudinger ligation: Attachment of an RGD motif either before or after complexation.

Molecular mechanism of target recognition by subtilin, a class I lanthionine antibiotic (2008)
Journal Article

The increasing resistance of human pathogens to conventional antibiotics presents a growing threat to the chemotherapeutic management of infectious diseases. The lanthionine antibiotics, still unused as therapeutic agents, have recently attracted sig... Read More about Molecular mechanism of target recognition by subtilin, a class I lanthionine antibiotic.

Look who's talking: Communication and quorum sensing in the bacterial world (2007)
Journal Article

For many years bacteria were considered primarily as autonomous unicellular organisms with little capacity for collective behaviour. However, we now appreciate that bacterial cells are in fact, highly communicative. The generic term 'quorum sensing'... Read More about Look who's talking: Communication and quorum sensing in the bacterial world.

Amino acids (2006)
Book Chapter

Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus (2006)
Journal Article

Lysostaphin (EC. 3.4.24.75) is a protein secreted by Staphylococcus simulans biovar staphylolyticus and has been shown to be active against methicillin resistant S. aureus (MRSA). The design and synthesis of three internally quenched substrates for l... Read More about Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus.

Antigenicity of chimeric and cyclic synthetic peptides based on nonstructural proteins of GBV-C/HGV (2005)
Journal Article

In this work, new putative epitopes located in nonstructural proteins of GBV-C/HGV were synthesized using solid-phase chemistry for their use in immunoassays. The antigens were obtained in linear, chimeric and cyclic forms with the main aim of improv... Read More about Antigenicity of chimeric and cyclic synthetic peptides based on nonstructural proteins of GBV-C/HGV.

Discovery of antagonist peptides against bacterial helicase-primase interaction in B. stearothermophilus by reverse yeast three-hybrid (2005)
Journal Article

Developing small-molecule antagonists against protein-protein interactions will provide powerful tools for mechanistic/functional studies and the discovery of new antibacterials. We have developed a reverse yeast three-hybrid approach that allows hig... Read More about Discovery of antagonist peptides against bacterial helicase-primase interaction in B. stearothermophilus by reverse yeast three-hybrid.

Modulation of pRb/E2F functions in the regulation of cell cycle and in cancer (2005)
Journal Article

Cell proliferation is regulated by the cell cycle, and in order to divide the cell must enter a mitotic state. Prior to mitosis the cell is required to pass through a number of checkpoints, including the critical G1/S restriction point governed by th... Read More about Modulation of pRb/E2F functions in the regulation of cell cycle and in cancer.

Atomic force microscopy study of human amylin (20-29) fibrils (2005)
Journal Article

Here we present atomic force microscopy images of the fibrils formed by human amylin(20-29). This peptide is a fragment of the polypeptide amylin, the major proteinaceous component of amyloid deposits found in cases of type-II diabetes mellitus. Our... Read More about Atomic force microscopy study of human amylin (20-29) fibrils.

Virulence regulation and quorum sensing in staphylococcal infections: Competitive AgrC antagonists as quorum sensing inhibitors (2004)
Journal Article

Expedient synthesis of a novel class of pseudoaromatic amino acids: Tetrahydroindazol-3-yl- and tetrahydrobenzisoxazol-3-ylalanine derivatives (2003)
Journal Article

A concise synthesis of novel homochiral aromatic amino acid surrogates comprising a tetrahydroindazole or a benzisoxazole system was developed via the acylation of a cyclic 1,3-diketone by the side-chain carboxyl functionality of either Boc-Asp-OtBu... Read More about Expedient synthesis of a novel class of pseudoaromatic amino acids: Tetrahydroindazol-3-yl- and tetrahydrobenzisoxazol-3-ylalanine derivatives.

Side-chain-to-tail thiolactone peptide inhibitors of the staphylococcal quorum-sensing system (2003)
Journal Article

The expression of many staphylococcal virulence factors are regulated by the agr locus via a two-component signal transduction system (TCSTS), which is activated in response to a secreted autoinducer peptide (AIP). By exploiting the unique chemical a... Read More about Side-chain-to-tail thiolactone peptide inhibitors of the staphylococcal quorum-sensing system.