5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: A robust method to screen MAPK pathway allosteric inhibitors

Rezvani, Z. Nooshin; Mayer, R. John; Chan, Weng C.

Abstract

Fluorescence polarization using Nα-(fluorescein-5- carbonyl)-Nε-(N-[2-fluoro-4-iodophenyl]-3,4- difluoroanthraniloyl)lysyl amide is capable of rapidly identifying inhibitor ligands that bind to an allosteric site in MEK1. © The Royal Society of Chemistry.

• Journal Article Type: Article
• Acceptance Date: Jan 27, 2010
• Online Publication Date: Feb 12, 2010
• Publication Date: Mar 19, 2010
• Deposit Date: Sep 10, 2020
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 46
• Issue: 12
• Pages: 2043-2045
• DOI: https://doi.org/10.1039/b925042e
• Public URL: https://nottingham-repository.worktribe.com/output/3137691
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2010/CC/b925042e#!divAbstract