Targeting of polyamidoamine-DNA nanoparticles using the Staudinger ligation: Attachment of an RGD motif either before or after complexation

Parkhouse, Susan M.; Garnett, Martin C.; Chan, Weng C.

Abstract

Two new methods for the modular synthesis of targeted gene delivery systems are reported. The PEGylated polyamidoamine DMEDA-PEG-DMEDA-(MBA-DMEDA)n+1-PEG-DMEDA 3 was sequentially modified to contain an integrin-binding peptide ligand via the Staudinger ligation. The conjugation of the ligand was achieved either before particle complexation (precomplexation) or after particle complexation (postcomplexation). Comparison of the two systems showed that postcomplexation strategy led to small and discrete toroidal nanoparticles whilst the precomplexation particles showed loose complexes. The targeted particles showed an increased uptake into cells compared to unmodified complexes however no significant increase in transfection was seen. © 2008 Elsevier Ltd. All rights reserved.

Citation

Parkhouse, S. M., Garnett, M. C., & Chan, W. C. (2008). Targeting of polyamidoamine-DNA nanoparticles using the Staudinger ligation: Attachment of an RGD motif either before or after complexation. Bioorganic and Medicinal Chemistry, 16(13), 6641-6650. https://doi.org/10.1016/j.bmc.2008.05.023

• Journal Article Type: Article
• Acceptance Date: May 7, 2008
• Online Publication Date: May 10, 2008
• Publication Date: Jul 1, 2008
• Deposit Date: Sep 10, 2020
• Journal: Bioorganic and Medicinal Chemistry
• Print ISSN: 0968-0896
• Electronic ISSN: 1464-3391
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 13
• Pages: 6641-6650
• DOI: https://doi.org/10.1016/j.bmc.2008.05.023
• Public URL: https://nottingham-repository.worktribe.com/output/3137719
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0968089608004525