All Outputs (12)

Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

Halofrax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems (2019)
Journal Article

Enzyme-mediated synthesis of pharmaceutical compounds is a ‘green’ alternative to traditional synthetic chemistry, and microbial engineering opens up the possibility of using whole cells as mini-factories. Whole-cell biocatalysis reduces cost by elim... Read More about Halofrax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems.

Haloferax volcanii for biotechnology applications: challenges, current state and perspectives (2019)
Journal Article

© 2019, The Author(s). Haloferax volcanii is an obligate halophilic archaeon with its origin in the Dead Sea. Simple laboratory culture conditions and a wide range of genetic tools have made it a model organism for studying haloarchaeal cell biology.... Read More about Haloferax volcanii for biotechnology applications: challenges, current state and perspectives.

DNA repair in the archaea: an emerging picture (2018)
Journal Article

There has long been a fascination in the DNA Repair pathways of archaea, for two main reasons. Firstly, many archaea inhabit extreme environments where the rate of physical damage to DNA is accelerated. These archaea might reasonably be expected to h... Read More about DNA repair in the archaea: an emerging picture.

Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii (2018)
Journal Article

The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evol... Read More about Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii.

RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator (2017)
Journal Article

Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome insta... Read More about RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator.

High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon (2017)
Journal Article

CRISPR-Cas systems allow bacteria and archaea to acquire sequence-specific immunity against selfish genetic elements such as viruses and plasmids, by specific degradation of invader DNA or RNA. However, this involves the risk of autoimmunity if immun... Read More about High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.

Diversity of DNA replication in the archaea (2017)
Journal Article

DNA replication is arguably the most fundamental biological process. On account of their shared evolutionary ancestry, the replication machinery found in archaea is similar to that found in eukaryotes. DNA replication is initiated at origins and is h... Read More about Diversity of DNA replication in the archaea.

Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor (2015)
Journal Article

The success of biotechnological processes is based on the availability of efficient and highly specific biocatalysts, which can satisfy industrial demands. Extreme and remote environments like the deep brine pools of the Red Sea represent highly inte... Read More about Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor.

DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii (2013)
Journal Article

In prokaryotes the genome is organized in a dynamic structure called the nucleoid, which is embedded in the cytoplasm. We show here that in the archaeon Haloferax volcanii, compaction and reorganization of the nucleoid is induced by stresses that... Read More about DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii.

Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii (2012)
Journal Article

Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded ?-sheet coiled into a closed barrel, to bind to ssDNA thereby protecting a... Read More about Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii.

Archaeal genetics – the third way (2005)
Journal Article

For decades, archaea were misclassified as bacteria on account of their prokaryotic morphology. Molecular phylogeny eventually revealed that archaea, like bacteria and eukaryotes, are a fundamentally distinct domain of life. Genome analyses have conf... Read More about Archaeal genetics – the third way.