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DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii

Delmas, Stéphane; Duggin, Iain J.; Allers, Thorsten

Authors

Stéphane Delmas

Iain J. Duggin

Thorsten Allers



Abstract

In prokaryotes the genome is organized in a dynamic
structure called the nucleoid, which is embedded in
the cytoplasm. We show here that in the archaeon
Haloferax volcanii, compaction and reorganization of
the nucleoid is induced by stresses that damage the
genome or interfere with its replication. The fraction
of cells exhibiting nucleoid compaction was proportional
to the dose of the DNA damaging agent, and
results obtained in cells defective for nucleotide excision
repair suggest that breakage of DNA strands
triggers reorganization of the nucleoid. We observed
that compaction depends on the Mre11-Rad50
complex, suggesting a link to DNA double-strand
break repair. However, compaction was observed in a
radA mutant, indicating that the role of Mre11-Rad50
in nucleoid reorganisation is independent of homologous
recombination. We therefore propose that
nucleoid compaction is part of a DNA damage
response that accelerates cell recovery by helping
DNA repair proteins to locate their targets, and facilitating
the search for intact DNA sequences during
homologous recombination.

Journal Article Type Article
Publication Date Jan 1, 2013
Journal Molecular Microbiology
Print ISSN 0950-382X
Electronic ISSN 0950-382X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 87
Issue 1
APA6 Citation Delmas, S., Duggin, I. J., & Allers, T. (2013). DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii. Molecular Microbiology, 87(1), doi:10.1111/mmi.12091
DOI https://doi.org/10.1111/mmi.12091
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mmi.12091/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0



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