All Outputs (20)

NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2 (2024)
Journal Article

Background Testicular germ cell tumours (TGCTs) represent a clinical challenge; they are most prevalent in young individuals and are triggered by molecular mechanisms that are not fully understood. The origin of TGCTs can be traced back to primordia... Read More about NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2.

Interspecies control of development during mammalian gastrulation (2023)
Journal Article

Gastrulation represents a pivotal phase of development and aberrations during this period can have major consequences, from minor anatomical deviations to severe congenital defects. Animal models are used to study gastrulation, however, there is cons... Read More about Interspecies control of development during mammalian gastrulation.

NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl (2023)
Journal Article

Pluripotency defines the unlimited potential of individual cells of vertebrate embryos, from which all adult somatic cells and germ cells are derived. Understanding how the programming of pluripotency evolved has been obscured in part by a lack of da... Read More about NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl.

Review: A barnyard in the lab: prospect of generating animal germ cells for breeding and conservation (2023)
Journal Article

In vitro gametogenesis (IVG) offers broad opportunities for gaining detailed new mechanistic knowledge of germ cell biology that will enable progress in the understanding of human infertility, as well as for applications in the conservation of endang... Read More about Review: A barnyard in the lab: prospect of generating animal germ cells for breeding and conservation.

In preprints: towards reconstituting an ovary (2022)
Journal Article

In vitro culture of ovine embryos up to early gastrulating stages (2022)
Journal Article

Developmental failures occurring shortly after blastocyst hatching from the zona pellucida constitute a major cause of pregnancy losses in both humans and farm ungulates. The developmental events occurring following hatching in ungulates include the... Read More about In vitro culture of ovine embryos up to early gastrulating stages.

Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species (2021)
Journal Article

Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the em... Read More about Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.

Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells? (2021)
Journal Article

In vitro production of tissue-specific stem cells [e.g. haematopoietic stem cells (HSCs)] is a key goal of regenerative medicine. However, recent efforts to produce fully functional tissue-specific stem cells have fallen short. One possible cause of... Read More about Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?.

Nuclear transfer and the development of genetically modified/gene edited livestock (2021)
Journal Article

The birth and adult development of 'Dolly' the sheep, the first mammal produced by the transfer of a terminally differentiated cell nucleus into an egg, provided unequivocal evidence of nuclear equivalence among somatic cells. This ground-breaking ex... Read More about Nuclear transfer and the development of genetically modified/gene edited livestock.

Nuclear transfer and the development of genetically modified/gene edited livestock (2021)
Journal Article

The birth and adult development of 'Dolly' the sheep, the first mammal produced by the transfer of a terminally differentiated cell nucleus into an egg, provided unequivocal evidence of nuclear equivalence among somatic cells. This ground-breaking ex... Read More about Nuclear transfer and the development of genetically modified/gene edited livestock.

Conserved features of non-primate bilaminar disc embryos and the germline (2021)
Journal Article

Post-implantation embryo development commences with a bilaminar disc in most mammals, including humans. Whereas access to early human embryos is limited and subject to greater ethical scrutiny, studies on non-primate embryos developing as bilaminar d... Read More about Conserved features of non-primate bilaminar disc embryos and the germline.

Regulation of Cell Fate Decisions in Early Mammalian Embryos (2019)
Journal Article

Early embryogenesis is characterized by the segregation of cell lineages that fulfill critical roles in the establishment of pregnancy and development of the fetus. The formation of the blastocyst marks the emergence of extraembryonic precursors, nee... Read More about Regulation of Cell Fate Decisions in Early Mammalian Embryos.

A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos (2019)
Journal Article

Background The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence s... Read More about A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos.

Principles of early human development and germ cell program from conserved model systems (2017)
Journal Article

Human primordial germ cells (hPGCs), the precursors of sperm and eggs, originate during week 2-3 of early postimplantation development(1). Using in vitro models of hPGC induction(2-4), recent studies suggest striking mechanistic differences in specif... Read More about Principles of early human development and germ cell program from conserved model systems.

Epigenetics and developmental programming of welfare and production traits in farm animals (2016)
Journal Article

The concept that postnatal health and development can be influenced by events that occur in utero originated from epidemiological studies in humans supported by numerous mechanistic (including epigenetic) studies in a variety of model species. Referr... Read More about Epigenetics and developmental programming of welfare and production traits in farm animals.

Primordial germ cells: the first cell lineage or the last cells standing? (2015)
Journal Article

Embryos of many animal models express germ line determinants that suppress transcription and mediate early germ line commitment, which occurs before the somatic cell lineages are established. However, not all animals segregate their germ line in this... Read More about Primordial germ cells: the first cell lineage or the last cells standing?.

Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes (2015)
Journal Article

Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerate... Read More about Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes.

Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer (2015)
Journal Article

Induced pluripotent stem cells (iPSCs) share similar characteristics of indefinite in vitro growth with embryonic stem cells (ESCs) and may therefore serve as a useful tool for the targeted genetic modification of farm animals via nuclear transfer (N... Read More about Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer.

Differential acetylation of histone H4 lysine during development of in vitro fertilized, cloned and parthenogenetically activated bovine embryos (2008)
Journal Article

The oocyte is remarkable in its ability to remodel parental genomes following fertilization and to reprogram somatic nuclei after nuclear transfer (NT). To characterize the patterns of histone H4 acetylation and DNA methylation during development of... Read More about Differential acetylation of histone H4 lysine during development of in vitro fertilized, cloned and parthenogenetically activated bovine embryos.

Cloning: Eight years after Dolly (2005)
Journal Article