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Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer

German, Sergio D.; Campbell, Keith H.S.; Thornton, Elisabeth; McLachlan, Gerry; Sweetman, Dylan; Alberio, Ramiro

Authors

Sergio D. German

Keith H.S. Campbell

Elisabeth Thornton

Gerry McLachlan

Dylan Sweetman

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RAMIRO ALBERIO ramiro.alberio@nottingham.ac.uk
Professor of Developmental Biology



Abstract

Induced pluripotent stem cells (iPSCs) share similar characteristics of indefinite in vitro growth with embryonic stem cells (ESCs) and may therefore serve as a useful tool for the targeted genetic modification of farm animals via nuclear transfer (NT). Derivation of stable ESC lines from farm animals has not been possible, therefore, it is important to determine whether iPSCs can be used as substitutes for ESCs in generating genetically modified cloned farm animals. We generated ovine iPSCs by conventional retroviral transduction using the four Yamanaka factors. These cells were basic fibroblast growth factor (bFGF)- and activin A-dependent, showed persistent expression of the transgenes, acquired chromosomal abnormalities, and failed to activate endogenous NANOG. Nonetheless, iPSCs could differentiate into the three somatic germ layers in vitro. Because cloning of farm animals is best achieved with diploid cells (G1/G0), we synchronized the iPSCs in G1 prior to NT. Despite the cell cycle synchronization, preimplantation development of iPSC-NT embryos was lower than with somatic cells (2% vs. 10% blastocysts, p

Citation

German, S. D., Campbell, K. H., Thornton, E., McLachlan, G., Sweetman, D., & Alberio, R. (2015). Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer. Cellular Reprogramming, 17(1), https://doi.org/10.1089/cell.2014.0071

Journal Article Type Article
Publication Date Feb 1, 2015
Deposit Date Jul 21, 2015
Publicly Available Date Mar 29, 2024
Journal Cellular Reprogramming
Print ISSN 2152-4971
Electronic ISSN 2152-4971
Publisher Mary Ann Liebert
Peer Reviewed Peer Reviewed
Volume 17
Issue 1
DOI https://doi.org/10.1089/cell.2014.0071
Public URL https://nottingham-repository.worktribe.com/output/985078
Publisher URL http://www.liebertpub.com/overview/cellular-reprogramming/9/
Additional Information Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/cell.2014.0071.

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