Sergio D. German
Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer
German, Sergio D.; Campbell, Keith H.S.; Thornton, Elisabeth; McLachlan, Gerry; Sweetman, Dylan; Alberio, Ramiro
Authors
Keith H.S. Campbell
Elisabeth Thornton
Gerry McLachlan
Dylan Sweetman
RAMIRO ALBERIO ramiro.alberio@nottingham.ac.uk
Professor of Developmental Biology
Abstract
Induced pluripotent stem cells (iPSCs) share similar characteristics of indefinite in vitro growth with embryonic stem cells (ESCs) and may therefore serve as a useful tool for the targeted genetic modification of farm animals via nuclear transfer (NT). Derivation of stable ESC lines from farm animals has not been possible, therefore, it is important to determine whether iPSCs can be used as substitutes for ESCs in generating genetically modified cloned farm animals. We generated ovine iPSCs by conventional retroviral transduction using the four Yamanaka factors. These cells were basic fibroblast growth factor (bFGF)- and activin A-dependent, showed persistent expression of the transgenes, acquired chromosomal abnormalities, and failed to activate endogenous NANOG. Nonetheless, iPSCs could differentiate into the three somatic germ layers in vitro. Because cloning of farm animals is best achieved with diploid cells (G1/G0), we synchronized the iPSCs in G1 prior to NT. Despite the cell cycle synchronization, preimplantation development of iPSC-NT embryos was lower than with somatic cells (2% vs. 10% blastocysts, p
Citation
German, S. D., Campbell, K. H., Thornton, E., McLachlan, G., Sweetman, D., & Alberio, R. (2015). Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer. Cellular Reprogramming, 17(1), https://doi.org/10.1089/cell.2014.0071
Journal Article Type | Article |
---|---|
Publication Date | Feb 1, 2015 |
Deposit Date | Jul 21, 2015 |
Publicly Available Date | Mar 29, 2024 |
Journal | Cellular Reprogramming |
Print ISSN | 2152-4971 |
Electronic ISSN | 2152-4971 |
Publisher | Mary Ann Liebert |
Peer Reviewed | Peer Reviewed |
Volume | 17 |
Issue | 1 |
DOI | https://doi.org/10.1089/cell.2014.0071 |
Public URL | https://nottingham-repository.worktribe.com/output/985078 |
Publisher URL | http://www.liebertpub.com/overview/cellular-reprogramming/9/ |
Additional Information | Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/cell.2014.0071. |
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