All Outputs (24)

Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids (2024)
Patent
Webb, K., Moradi, E., & Foss, A. (2024). Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids. WO2024009101A1. UK

The invention provides a method of delivering a payload molecule across an epithelial tissue barrier, the method comprising: applying the payload molecule to the epithelial tissue barrier, and additionally applying an agent to the epithelial tissue b... Read More about Disruption of Epithelial Barriers for Molecular Delivery Enhancement or Extraction of Extracellular Fluids.

Investigation of Exhaust Particles on Different TEM Grids: a Comparison between Graphene Oxide and Silicon Nitride Grids (2023)
Presentation / Conference Contribution
Lagana, S., Akifjevs, R., Rocca, A. L., Cairns, A., Fay, M. W., & Webb, K. F. (2023). Investigation of Exhaust Particles on Different TEM Grids: a Comparison between Graphene Oxide and Silicon Nitride Grids. SAE Technical Papers, 2023-32-0123, https://doi.org/10.4271/2023-32-0123

Two different TEM (Transmission Electron Microscopy) grids - graphene oxide (GO) and silicon nitride (SiN) - were used to capture the particulates emitted with the exhaust of a modern 1.0 L GDI (Gasoline Direct Injection) engine. One speed-load condi... Read More about Investigation of Exhaust Particles on Different TEM Grids: a Comparison between Graphene Oxide and Silicon Nitride Grids.

The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro (2022)
Journal Article
McCloskey, M. C., Kasap, P., Ahmad, S. D., Su, S., Chen, K., Mansouri, M., …McGrath, J. L. (2022). The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro. Advanced Healthcare Materials, 11(18), Article 2200804. https://doi.org/10.1002/adhm.202200804

Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support "on-chip" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow... Read More about The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro.

Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro (2020)
Journal Article
Harun, M., Marsh, V., Elsaied, N. A., Webb, K. F., & Elsheikha, H. M. (2020). Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro. Brain Research, 1746, Article 147002. https://doi.org/10.1016/j.brainres.2020.147002

Toxoplasma gondii can cause parasitic encephalitis, a life-threatening infection that predominately occurs in immunocompromised individuals. T. gondii has the ability to invade the brain, but the mechanisms by which this parasite crosses the blood–br... Read More about Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro.

Surface plasmon resonance imaging of excitable cells (2019)
Journal Article
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. (2019). Surface plasmon resonance imaging of excitable cells. Journal of Physics D: Applied Physics, 52(10), Article 104001. https://doi.org/10.1088/1361-6463/aaf849

Surface plasmons (SPs) are surface charge density oscillations occuring at a metal/dieletric interface and are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and... Read More about Surface plasmon resonance imaging of excitable cells.

Surface Plasmon Resonance Imaging of Excitable Cells (2018)
Preprint / Working Paper
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. Surface Plasmon Resonance Imaging of Excitable Cells

Surface plasmons are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detect... Read More about Surface Plasmon Resonance Imaging of Excitable Cells.

TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN) (2018)
Journal Article
Madejski, G., Lucas, K., Pascut, F. C., Webb, K. F., & McGrath, J. L. (2018). TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN). Membranes, 8(2), Article 26. https://doi.org/10.3390/membranes8020026

Silicon nanomembrane technologies (NPN, pnc-Si, and others) have been used commercially as electron microscopy (EM) substrates, and as filters with nanometer-resolution size cut-offs. Combined with EM, these materials provide a platform for catching... Read More about TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN).

Non destructive evaluation of biological cells (2018)
Presentation / Conference Contribution
Pérez-Cota, F., Smith, R. J., Moradi, E., Webb, K. F., & Clark, M. (2019). Non destructive evaluation of biological cells. AIP Conference Proceedings, 2102, https://doi.org/10.1063/1.5099737

© 2019 Author(s). Regenerative medicine promises to be the next revolution in health care. This technology, which will be the first systematic manufacturing of biological parts for human consumption, requires non destructive evaluatioin (NDE) thechni... Read More about Non destructive evaluation of biological cells.

Rapid one-step biotinylation of biological and non-biological surfaces (2018)
Journal Article
Henry, S., Williams, E., Barr, K., Korchagina, E., Tuzikov, A., Ilyushina, N., …Bovin, N. (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8(1), Article 2845. https://doi.org/10.1038/s41598-018-21186-3

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minu... Read More about Rapid one-step biotinylation of biological and non-biological surfaces.

Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells (2018)
Journal Article
Petrova, R. S., Webb, K. F., Vaghefi, E., Walker, K., Schey, K. L., & Donaldson, P. J. (2018). Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells. American Journal of Physiology - Cell Physiology, 314(2), C191-C201. https://doi.org/10.1152/ajpcell.00214.2017

Although the functionality of the lens water channels aquaporin 1 (AQP1; epithelium) and AQP0 (fiber cells) is well established, less is known about the role of AQP5 in the lens. Since in other tissues AQP5 functions as a regulated water channel with... Read More about Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells.

Sensitive detection of voltage transients using differential intensity surface plasmon resonance system (2017)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2017). Sensitive detection of voltage transients using differential intensity surface plasmon resonance system. Optics Express, 25(25), 31552-31567. https://doi.org/10.1364/OE.25.031552

This paper describes theoretical and experimental study of the fundamentals of using surface plasmon resonance (SPR) for label-free detection of voltage. Plasmonic voltage sensing relies on the capacitive properties of metal-electrolyte interface tha... Read More about Sensitive detection of voltage transients using differential intensity surface plasmon resonance system.

Responsivity of the differential-intensity surface plasmon resonance instrument (2016)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2016). Responsivity of the differential-intensity surface plasmon resonance instrument. Sensors and Actuators B: Chemical, 235, 627-635. https://doi.org/10.1016/j.snb.2016.05.117

Surface plasmon resonance is used for the sensitive measurement of minute concentrations of bio-analytes and probing of electrochemical processes. Typical refractive index sensitivity, for the intensity approach, is around 10−6 refractive index units... Read More about Responsivity of the differential-intensity surface plasmon resonance instrument.

Cell imaging using sub-optical wavelength ultrasonics (2015)
Presentation / Conference Contribution
Clark, M., Marques, L., Moradi, E., Perez-Cota, F., Smith, R. J., & Webb, K. F. (2015). Cell imaging using sub-optical wavelength ultrasonics. In Proceedings Asia Communications and Photonics Conference 2015. https://doi.org/10.1364/acpc.2015.asu5h.1

At GHz frequencies the wavelength of ultrasound becomes comparable or smaller than the wavelength of light and so offers an intriguing way to image at the small scale. In this paper we report on new developments in pico-second laser ultrasonics that... Read More about Cell imaging using sub-optical wavelength ultrasonics.

Condenser-free contrast methods for transmitted-light microscopy (2014)
Journal Article
Webb, K. F. (2014). Condenser-free contrast methods for transmitted-light microscopy. Journal of Microscopy, 257(1), 8-22. https://doi.org/10.1111/jmi.12181

Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser opt... Read More about Condenser-free contrast methods for transmitted-light microscopy.

Sound of nano (2013)
Presentation / Conference Contribution
Aylott, J., Clark, M., Marques, L., Perez-Cota, F., Smith, R. J., & Webb, K. F. (2013). Sound of nano. Proceedings of SPIE, 8923, Article 892325. https://doi.org/10.1117/12.2035176

Ultrasound is widely used for imaging, measurement and diagnostics in the MHz region and is perhaps most familiar as a medical or non-destructive imaging or measurement tool. In the MHz frequency range the wavelength is typically measured in microns... Read More about Sound of nano.

Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage (2012)
Journal Article
Gunning, S. J., Chung, K. K., Donaldson, P. J., & Webb, K. F. (2012). Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. American Journal of Physiology - Cell Physiology, 303(12), C1252-C1259. https://doi.org/10.1152/ajpcell.00237.2012

The initiation of lens cataract has long been associated with the development of a membrane "leak" in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that... Read More about Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage.

Multimodal imaging of fluid transport in living epithelial sheets (2011)
Presentation / Conference Contribution
Webb, K. F., Zhang, J., & Somekh, M. (2011). Multimodal imaging of fluid transport in living epithelial sheets. In 2011 Functional Optical Imaging. https://doi.org/10.1109/FOI.2011.6154825

Detailed knowledge on the biophysical mechanisms and local intercompartmental dynamics by which fluid and solute transport is achieved and regulated at the cellular and subcellular level is lacking. In this paper, cultures of immortalised human retin... Read More about Multimodal imaging of fluid transport in living epithelial sheets.

Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation (2011)
Journal Article
Mendoza-Naranjo, A., Bouma, G., Pereda, C., Ramiŕez, M., Webb, K. F., Tittarelli, A., …Salazar-Onfray, F. (2011). Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation. Journal of Immunology, 187(6), 3121-3132. https://doi.org/10.4049/jimmunol.1100378

Gap junction (GJ) mediates intercellular communication through linked hemichannels from each of two adjacent cells. Using human and mouse models, we show that connexin 43 (Cx43), the main GJ protein in the immune system, was recruited to the immunolo... Read More about Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation.

Ionic Permeability and Currents in the Lens (2010)
Book Chapter
Donaldson, P. J., & Webb, K. F. (2010). Ionic Permeability and Currents in the Lens. In Encyclopedia of the Eye (477-486). https://doi.org/10.1016/B978-0-12-374203-2.00032-4

© 2010 Elsevier Ltd. All rights reserved. It has been proposed that in the absence of a blood supply the lens operates an internal microcirculation system. In this system spatially distinct differences in the membrane transport properties of the lens... Read More about Ionic Permeability and Currents in the Lens.

Regulation of lens volume: Implications for lens transparency (2009)
Journal Article
Donaldson, P. J., Chee, K. S. N., Lim, J. C., & Webb, K. F. (2009). Regulation of lens volume: Implications for lens transparency. Experimental Eye Research, 88(2), 144-150. https://doi.org/10.1016/j.exer.2008.05.011

Lens transparency is critically dependent on the maintenance of an ordered tissue architecture, and disruption of this order leads to light scatter and eventually lens cataract. Hence the volume of the fiber cells that make up the bulk of the lens ne... Read More about Regulation of lens volume: Implications for lens transparency.